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Leech extract and its preparation method and use

A technology of extract and leech, applied in the field of leech extract and its preparation, can solve the problems of in-depth research and application of less active protein peptides, etc., and achieve the effect of prolonging the time of plasma recalcification, improving the depth of research, and the direction of simple active ingredients

Inactive Publication Date: 2013-04-24
SUN YAT SEN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] With the in-depth research on the active ingredients of leeches in modern times, it has been discovered that many protein polypeptides with anticoagulant activity are contained in leeches, but due to the particularity and complexity of the structure of protein polypeptides, the discovery of such active substances has brought great challenges. There are many challenges and difficulties. Except for the rich research on hirudin extracted from Japanese medical leeches, there are few in-depth studies and applications of other active protein peptides in the literature.

Method used

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  • Leech extract and its preparation method and use
  • Leech extract and its preparation method and use
  • Leech extract and its preparation method and use

Examples

Experimental program
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Effect test

Embodiment 1

[0022] The preparation method of embodiment 1 leech extract

[0023] Take the dried medicinal materials of leeches dried at 40°C for 18 hours, crush them (through a 60-mesh sieve), take 10g of medicinal powder, put them into a filter paper tube, connect a Soxhlet extractor, and pour 60ml of petroleum ether into a flask (60°C~90°C ), to 2 / 3 of the height of the siphon tube, connect the condenser tube and plug a little absorbent cotton on the top, turn on the cooling water, and turn on the 97°C water bath for heating, so that the petroleum ether in the extraction tube is in a slightly boiling state, and the reflux rate is controlled at 3 drops per second , extracted for 12 hours, the extract was vacuum spin-dried to recover petroleum ether, and finally 3.12 g of leech extract was obtained.

Embodiment 2

[0024] The preparation method of embodiment 2 leech extract

[0025] Take the dried medicinal materials of leeches dried at 35°C for 36 hours, crush them (through a 100-mesh sieve), take 10g of medicinal powder, put them into a filter paper tube, connect a Soxhlet extractor, and pour 40ml of petroleum ether (30°C~60°C) into a flask. ), to 2 / 3 of the height of the siphon tube, connect the condenser tube and plug a little absorbent cotton on the top, turn on the cooling water, and turn on the 97°C water bath to heat, so that the petroleum ether in the extraction tube is in a slightly boiling state, and control the reflux rate to 1 drop per second , extracted for 16 hours, and the extract was vacuum spin-dried to recover petroleum ether, and finally 2.9 g of leech extract was obtained.

Embodiment 3

[0026] The preparation method of embodiment 3 leech extract

[0027] Take the dried medicinal materials of leeches after drying at 45°C for 12 hours, crush them (pass through a 80-mesh sieve), take 10g of medicinal powder, put them into a filter paper tube, connect a Soxhlet extractor, and pour 80ml of petroleum ether into a flask (60°C~90°C ), to 2 / 3 of the height of the siphon tube, connect the condenser tube and plug a little absorbent cotton on the top, turn on the cooling water, and turn on the 97°C water bath for heating, so that the petroleum ether in the extraction tube is in a slightly boiling state, and the reflux rate is controlled at 2 drops per second , extracted for 6 hours, and the extract was vacuum spin-dried to recover petroleum ether, and finally 2.8 g of leech extract was obtained.

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Abstract

The invention discloses leech extract and its preparation method and use. The preparation method comprises adding petroleum ether into a leech medicinal material, wherein the volume of the petroleum ether is 4-8 times that of the leech medicinal material, carrying out backflow extraction for 6 to 16 hours to obtain extract, and recovering the petroleum ether from the extract so that the leech extract is obtained. The preparation method utilizes petroleum ether to carry out backflow extraction so that the aliphatic acid leech extract different from the traditional leech active protein polypeptide is obtained. The leech extract has an in-vitro anticoagulation effect.

Description

technical field [0001] The invention relates to a traditional Chinese medicine extract, in particular to a leech extract and its preparation method and application. Background technique [0002] Cardiovascular and cerebrovascular disease is a common disease that seriously threatens the health of human beings, especially middle-aged and elderly people over 50 years old, and has become the number one killer with the highest cause of death. At present, my country is gradually entering an aging society, and the demand for anticoagulant drugs is increasing. However, both traditional urea kinase and heparin have the disadvantage of bleeding after administration, which limits their clinical application. [0003] The dried body of leech (Whitmania pigra) is one of the varieties of leech medicinal materials recorded in the 2010 edition of the Pharmacopoeia of the People's Republic of China. my country has a long history of medicinal use, and it is a classic traditional Chinese medici...

Claims

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Application Information

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IPC IPC(8): A61K35/62A61P7/02
Inventor 钟山尹玉华杨得坡
Owner SUN YAT SEN UNIV
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