Taxus chinensis extractive with blood sugar reduction effect, as well as preparation method and application thereof
A yew extract and hypoglycemic technology, which is applied to medical preparations containing active ingredients, drug combinations, and pharmaceutical formulas, etc., can solve the problems of no diabetes drugs, and achieve convenient clinical application and high content of extracted active ingredients , cheap effect
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Embodiment 1
[0036] A yew extract with hypoglycemic effect, which is prepared by the following method:
[0037] 5 kg of branches and leaves of Taxus chinensis, cut into decoction pieces, extracted with 10 times the volume of 95% ethanol and 8 times 50% alcohol by reflux each time, combined the medicinal solution to concentrate the medicinal solution until it has no alcohol smell, and first extracted with petroleum ether to remove the pigment , and then extracted with ethyl acetate to obtain ethyl acetate extract; AB-8 type macroporous resin was applied to the ethyl acetate extract, eluted with distilled water and 0%-80% ethanol, and combined 60%-70% ethanol eluent Concentrate under reduced pressure and freeze-dry to obtain refined biflavonoids and phenolic acids.
[0038] Dissolve the effective part with 90% ethanol, and scan the sample at a wavelength of 350-600nm by ultraviolet spectrophotometry. There is maximum absorption at 504nm, and the measurement wavelength is determined to be 50...
Embodiment 2
[0040] A yew extract with hypoglycemic effect, which is prepared by the following method:
[0041]5 kg of branches and leaves of Taxus chinensis, cut into decoction pieces, extracted 3 times with 8 times the volume of 95% ethanol under reflux, combined the medicinal solution to concentrate the medicinal solution until it has no alcohol taste, first extracted with petroleum ether to remove the pigment, and then used ethyl acetate The ethyl acetate extract is extracted, and the ethyl acetate extract is separated by column chromatography on a polyamide column with a particle size of 15-60 mesh, and gradient elution is performed with ethanol with a concentration of 10-80%, to obtain refined biflavonoids and A mixture of phenolic acid compounds; the total flavone content measured by the method of Example 1 is 70%. The total flavonoids measured by HPLC contain five biflavones, which are Jinpine biflavanone, ginkgoxanthin, isoginkgoxanthin, ginkgo biflavanone, and spike flower biflav...
Embodiment 3
[0043] A yew extract with hypoglycemic effect, which is prepared by the following method:
[0044] 5 kg of southern yew branches and leaves, cut into decoction pieces, refluxed with 8 times the volume of 95% ethanol for three times, combined the liquid to concentrate the liquid until it has no alcohol taste, first extracted with petroleum ether to remove the pigment, and then with n-butanol Extract n-butanol extract. Put the n-butanol extract on the AB-8 type macroporous resin column, and carry out gradient elution with ethanol with a concentration of 50-80%, to obtain refined biflavonoids and glycoside mixtures; measure the total flavonoid content according to the method of Example 1 70%. The total flavonoids contained five biflavones and their glycosides were measured by HPLC, which were Jinpine biflavones and their glycosides, ginkgoxanthin and its glycosides, isoginkgoxanthin and its glycosides, ginkgo biflavones and their glycosides, and spike flower biflavones and its ...
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