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Application of total saponins of bacopa monnieri (L.) wettst. in preparation of medicaments for resisting cerebral ischemia

A technology of Bacopa monnieri and total saponins, applied in the field of traditional Chinese medicine extracts

Inactive Publication Date: 2013-04-24
SECOND MILITARY MEDICAL UNIV OF THE PEOPLES LIBERATION ARMY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

After inquiry, there is no report of this related drug before the present invention

Method used

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  • Application of total saponins of bacopa monnieri (L.) wettst. in preparation of medicaments for resisting cerebral ischemia
  • Application of total saponins of bacopa monnieri (L.) wettst. in preparation of medicaments for resisting cerebral ischemia
  • Application of total saponins of bacopa monnieri (L.) wettst. in preparation of medicaments for resisting cerebral ischemia

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Experimental program
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Effect test

Embodiment 1

[0037] Embodiment 1, the preparation of Bacopa monnieri total saponins

[0038] Take 10kg of Bacopa monniera medicinal material and add 1200kg of 40-70% ethanol to soak for 60min, decoct for 120min, repeat three times, combine the extract, filter to remove precipitation; Wash with deionized water to remove water-soluble impurities. Use 5 times the column volume of 40% ethanol, 5 times the column volume of 75% ethanol to elute successively, collect the 75% ethanol eluate; take the 75% ethanol eluate, evaporate the ethanol at low temperature until there is no alcohol smell, and concentrate to dryness , Dry under reduced pressure at 80°C for 24h to constant weight to obtain 70g of total saponins of Bacopa monnieri as a solid.

[0039] Bacopaside I, Bacoside A3, Bacopaside II, BacopasaponinC isomer and Bacopasaponin C five monomer standard preparations are prepared by liquid phase:

[0040]Take an appropriate amount of the total saponins of Bacopa monniera, add chromatographic p...

Embodiment 2

[0049] Embodiment 2, the application of total saponins of Bacopa monnieri in the preparation of medicines for the treatment of cerebral ischemic injury

[0050] The present invention adopts the middle cerebral artery occlusion model of thread embolism to cause acute cerebral ischemia-reperfusion injury model in rats, and adopts the prevention and treatment administration method of modeling on the third day of administration and continuous administration for six days, from the cerebral infarction area, The degree of cerebral edema and animal behavior scores proved the therapeutic effect of total saponins of Bacopa monnieri on acute cerebral ischemia-reperfusion injury.

[0051] 1. Experimental materials and methods

[0052] 1) Animals and groups:

[0053] SD male rats, weighing 260-280 g, were provided by the Experimental Animal Center of the Second Military Medical University. The animals were randomly divided into 5 groups: (1) sham operation group; (2) model group; (3) tot...

Embodiment 3

[0069] The preparation of embodiment 3, Bacopaside I

[0070] Bacopa monnieri is pulverized into powder 10kg after removing impurities, dissolved with methanol-water volume ratio 9:1, filtered, 90% methanol equilibrated Mitsubishi Chemical chiral MCI column on the filtrate, then eluted with 90% methanol, evaporated to dryness Get samples. After decolorization, the sample is mixed with silica gel in equal proportions and then prepared under medium pressure. The medium pressure conditions are: pressure 25bar, flow rate 6ml / min, water phase 70%-10%, methanol 30%-90%, total time 6.5h, of which 0.5 h is the equilibration column time. The samples obtained in Central Asia were compared with the standard by spot plate (developing agent: ethyl acetate: methanol: water = 20:3.4:2.7) to find the desired point, collected and evaporated to dryness. The extract obtained above was dissolved in equal volumes of chloroform and methanol, and passed through a gel column. The purified Bacopasi...

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Abstract

The invention provides an application of total saponins of bacopa monnieri (L.) wettst. in preparation of medicaments for resisting cerebral ischemia. The total saponins of the bacopa monnieri (L.) wettst. mainly comprise the following dammarane type triterpenoid saponin compounds: bacopaside I, bacoside A3, bacopaside II, bacopasaponin cisomer and bacopasaponin C. The invention further provides an application of the bacopaside I in preparation of a medicament for resisting cerebral ischemia. The experimental study shows that the total saponins of the bacopa monnieri (L.) wettst. and the bacopaside I have the effects of improving the behavioral symptoms caused by cerebral ischemia reperfusion injury, reducing the cerebral infarct volume and reducing the cerebral edema caused by cerebral ischemia of a rat. Therefore, the total saponins of the bacopa monnieri (L.) wettst. can be used for preparing the medicaments for preventing and treating cerebral ischemia. The medicaments for preventing and treating the cerebral ischemia are prepared from the total saponins of the bacopa monnieri (L.) wettst. or bacopaside I as the active component and medicinal accessories by adopting a conventional method. The invention further expands the function of the bacopaside I and the total saponins of the bacopa monnieri (L.) wettst. and provides the novel medicaments for preventing and treating the cerebral ischemia. The bacopaside I and the total saponins of the bacopa monnieri (L.) wettst. have high clinical application values.

Description

technical field [0001] The present invention relates to extracts of traditional Chinese medicines, in particular to the application of extracts of traditional Chinese medicines, in particular to the use of total saponins of Bacopa monnieri (Bacopa Monnieri (L.) Wettst) extracted and separated from Bacopa Monnieri (L.) Wettst in the preparation of anti-cerebral ischemia drugs in the application. Background technique [0002] Bacopa monnieri (L.) Wettst is the whole herb of Bacopa monnieri (L.) Wettst. The theory of traditional Chinese medicine describes that Bacopa monnieri has the effects of clearing heat and cooling blood, detoxifying and reducing swelling, and is used to treat dysentery, Red eyes and swelling, erysipelas, hemorrhoids, elephantiasis and other symptoms. [0003] The Indian Ayurvedic system of medicine believes that Bacopa monnieri can enhance intelligence and improve memory, and has the effects of delaying aging, preventing dementia, calming, hypnotizing, a...

Claims

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Application Information

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IPC IPC(8): A61K36/80A61K31/7048C07J71/00A61P9/10
Inventor 张卫东单磊刘芳刘晓军李元元苏娟沈云亨
Owner SECOND MILITARY MEDICAL UNIV OF THE PEOPLES LIBERATION ARMY
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