Method for extracting trichosanthes kirilowii maxim uranidin

An extraction method and technology of yellow pigment, which is applied in the field of extraction and preparation of natural plant pigments, can solve the problems of low extraction rate, difficult extraction, difficult dissolution of Trichosanthes yellow pigment, etc.

Inactive Publication Date: 2013-05-08
安阳天尊生物工程股份有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] In order to solve the problem that Trichosanthes yellow pigment is difficult to dissolve, difficult to extract, and the extraction rate is low, the invention provides a method for extracting Trichosanthes yellow pigment

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0013] Take Trichosanthes, remove seeds and skins, put Trichosanthes pulp into a food refiner and pulverize to obtain a feed liquid, weigh 10g of the feed liquid, add 150ml of deionized water, mix, and then use a mass concentration of 10% lemon Adjust the acid to pH = 4.0 to obtain a mixture, then add 0.05g of pectinase and 0.1g of cellulase, enzymatically hydrolyze for 3 hours at 50°C, vacuum filter, the filter cake is the pigment part, add 200ml Dissolve ethanol with a mass concentration of 95%, put it in a water bath, heat at 50°C for 90 minutes, filter to remove impurities, take 1ml of the filtrate, dilute it to 25ml with 95% ethanol, measure its absorbance A at 438nm =0.72, after the remaining filtrate was concentrated under reduced pressure to recover ethanol, it was vacuum-dried to obtain 1.1g of finished product, and the primary extraction rate was 11%.

[0014] The enzyme activity of the pectinase is 50,000 u / g.

[0015] The enzyme activity of the pectinase is 10,000...

Embodiment 2

[0017] Take Trichosanthes, peel, put Trichosanthes pulp into a food refiner and pulverize to obtain a feed liquid, weigh 10g of the feed liquid, add 150ml deionized water, mix, and then adjust to 10% citric acid with a mass concentration of pH value = 5.3, get the mixture, then add 0.03g pectinase and 0.07g cellulase, enzymolysis under the condition of 60 ℃ for 3 hours, vacuum filtration, the filter cake is the pigment part, add 200ml mass concentration Dissolve 95% ethanol, put it in a water bath, heat at 50°C for 90 minutes, filter to remove impurities, take 1ml of the filtrate, dilute it to 25ml with 95% ethanol, measure its absorbance at 438nm, A=0.65, The remaining filtrate was concentrated under reduced pressure to recover ethanol, and then vacuum-dried to obtain 0.89 g of the finished product, with a primary extraction rate of 8.9%.

[0018] The enzyme activity of the pectinase is 50,000 u / g.

[0019] The enzyme activity of the pectinase is 10,000 u / g.

Embodiment 3

[0021] Take Trichosanthes, remove seeds and skins, put Trichosanthes pulp into a food refiner and pulverize to obtain a feed liquid, weigh 10g of the feed liquid, add 150ml of deionized water, mix, and then use a mass concentration of 10% lemon Adjust the pH value to 6.0 with acid, then add 0.01g pectinase and 0.03g cellulase, enzymatically hydrolyze for 3 hours at 55°C, vacuum filter, the filter cake is the pigment part, add 200ml mass concentration of 95 % ethanol, put it in a water bath, heat at 50°C for 90 minutes, filter to remove impurities, filter, take 1ml of the filtrate, dilute it to 25ml with 95% ethanol, measure its absorbance at 438nm A=0.66 , the remaining filtrate was concentrated under reduced pressure, and dried in vacuo to obtain 0.92 g of the finished product, with a primary extraction rate of 9.2%.

[0022] The enzyme activity of the pectinase is 50,000 u / g.

[0023] The enzyme activity of the pectinase is 10,000 u / g.

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Abstract

The invention relates to a method for extracting trichosanthes kirilowii maxim uranidin, and solves the problems of dissolving difficulty, extracting difficulty and low extraction rate of the trichosanthes kirilowii maxim uranidin. The method comprises the steps of taking trichosanthes kirilowii maxim, removing seeds and skin; crushing trichosanthes kirilowii crumb, adding deionized water to mix; adjusting a pH value by citric acid; adding pectinase and cellulose; carrying out enzymolysis, filtering in vacuum to obtain a filter cake; adding ethanol to dissolve; bathing in water for 90 minutes at 50 DEG C; filtering to obtain filtrate; concentrating the filtrate by reduced pressure and drying in vacuum. The cell walls of colloid and cellulose are destroyed by a two-enzyme method, so that the uranidin in each cell can be released, so as to easily extract and improve the yield. The viscosity of the extracting solution can be reduced, and the extracting solution is easy to filter.

Description

technical field [0001] The invention belongs to the technical field of extraction and preparation of natural plant pigments, and in particular relates to a method for extracting trichosanthes yellow pigment. Background technique [0002] Trichosanthes is a perennial herbaceous vine of Cucurbitaceae, distributed in most parts of the country. The yellow pigment containing lutein and carotene in ripe fruit has strong antioxidant capacity, and it is also the raw material of drugs for treating eye fundus lesions. The research and development of natural, safe and multifunctional pigments is the development of the food and pharmaceutical industries. direction. [0003] Castanopsis has been widely planted in my country from ancient times to the present, and its fruits, seeds and roots can be used alone for medicinal purposes. The skin of the ripe fruit is sweet in taste and cold in nature, and has the effects of nourishing the lungs and resolving phlegm, reducing fire and relievin...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C09B61/00
Inventor 杨保成谢书轩张晓忠
Owner 安阳天尊生物工程股份有限公司
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