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Method for testing the severity of an illness

An inspection method and severe disease technology, applied in the direction of disease diagnosis, biochemical equipment and methods, microbial measurement/inspection, etc., can solve the problems of many measurement items and time-consuming

Inactive Publication Date: 2013-05-08
UNIVERSITY OF TOKUSHIMA
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, the APACHE II score and SOFA score have many measurement items, and it takes time to summarize the evaluation, such as the measurement of urine volume per day, so they are not real-time markers, and an evaluation method that replaces the above scores needs to be established

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  • Method for testing the severity of an illness
  • Method for testing the severity of an illness
  • Method for testing the severity of an illness

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Embodiment

[0059] In order to facilitate the understanding of the present invention, the present invention will be specifically described below with reference examples and examples, but the present invention is of course not limited to the examples.

reference example 1

[0060] (Reference Example 1) Study on ATP Amount, Age, and Sex Distribution in Samples Derived from Healthy Persons

[0061] In this reference example, when establishing the method for examining the degree of disease severity of the present invention, in order to grasp the amount of ATP in samples derived from healthy people, a total of 139 samples were collected from 68 males and 71 females aged from their 20s to their 90s. The amount of ATP and lactic acid in the peripheral venous blood of healthy volunteers were measured.

[0062] Using the XL-ATP Kit (manufactured by Aprosyence Co., Ltd.), ATP was extracted from the sample according to the instruction manual, and the amount of ATP was measured. As the reagent for ATP extraction, the extraction reagent A (TE saturated phenol, composition: containing 69% phenol, pH 8.0), extraction reagent B (chloroform, composition: containing 99% chloroform) and sterile ultra- A reagent obtained by mixing pure water at a ratio of 3:5:5. ...

reference example 2

[0068] (Reference Example 2) Study on lactic acid content, age, and sex distribution in samples derived from healthy individuals

[0069] In this reference example, the amount of lactic acid in peripheral venous blood of a total of 139 healthy volunteers shown in Reference Example 1 was measured in order to grasp the amount of lactic acid in samples derived from healthy individuals. In the measurement of the amount of lactic acid, an automatic blood gas measurement device (860COT; Bayer Medical Co., Ltd.) or a simple measurement device (Lactite Pro; ARKRAY Co., Ltd.) was used to measure according to the measurement method recommended by the manufacturer.

[0070] The measured value (mM) of the amount of lactic acid in the sample derived from healthy people shown in Table 1 and Table 2 and its age and sex distribution are shown in figure 2 middle. A total of 139 healthy volunteers had no statistically significant differences in the lactic acid values ​​of peripheral venous bl...

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Abstract

The disclosed testing method for measuring the severity of an illness in real-time can assess the severity of an illness in more detail than the existing APACHE II and SOFA scores. Said method can accurately measure the amount of ATP in a sample and accurately and quickly deduce the "state of intracellular energy, which organisms need in order to live" from the amount of ATP, and thus can evaluate the severity of an illness. Furthermore, the ATP concentration is used as an index to re-evaluate the amount of lactic acid accumulated in the sample as a result of breakdowns in metabolic balance accompanying increasing disease severity (specifically, the lactic acid level (mM) is divided by the ATP concentration (mM)), thereby providing a novel biomarker, the ATP-Lactate Energy Risk Score (A-LES), that can assess the severity of an illness. Additionally, the ATP concentration is used as an index to re-evaluate the ketone body level in the sample (specifically, the ketone body level (mM) is divided by the ATP concentration (mM)), thereby providing a novel biomarker, the ATP-Ketone Energy Risk Score (A-KES), that can assess the severity of an illness.

Description

technical field [0001] The present invention relates to a method for examining the severity of a disease by measuring the amount of adenosine triphosphate (hereinafter referred to as "ATP") contained in a sample. In addition, the present invention also relates to a method for checking the severity of a disease by measuring the amount of ATP in a sample and the amount of lactic acid or ketone bodies, which are intermediate metabolites of energy metabolism, to grasp the state of energy production. Background technique [0002] ATP is a chemical substance (nucleotide) that all living things use as energy for survival. Therefore, measurement of ATP has been widely used to determine the presence or absence of microorganisms contained in a biological sample. In addition, since ATP is mainly produced in the mitochondria of intracellular organelles, ATP has been previously measured for the purpose of measuring the function of mitochondria. However, none of the existing ATP measure...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N33/50C12Q1/68G01N33/48
CPCC12Q1/68G01N2800/00G01N33/5735G01N33/48G01N33/50
Inventor 木户博西村匡司千田淳司
Owner UNIVERSITY OF TOKUSHIMA
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