Application of purslane alkaloid monomeric compound in preparation of anti-tumor medicament

An anti-tumor drug, alkaloid technology, applied in the field of medicine, can solve the problem that the anti-tumor activity has not been reported in literature and so on

Active Publication Date: 2013-05-22
GENERAL HOSPITAL OF THE NORTHERN WAR ZONE OF THE CHINESE PEOPLES LIBERATION ARMY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Its structure is as follows, but so far, the antitumor activity of these two monomeric compounds and their application as anticancer drugs have not been reported in the literature

Method used

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  • Application of purslane alkaloid monomeric compound in preparation of anti-tumor medicament

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0018] Example 1: Extraction, separation and structural identification of purslane amide B and orange piperonamide.

[0019] Purslane medicinal material (about 100 kg) was purchased, stirred and extracted with absolute ethanol 3 times, each time for 1 hour, and the ethanol was recovered to obtain a black extract. Add 10% sulfuric acid to the extract to dissolve and filter. The pH was adjusted to 10.0 with ammonia water, and a saturated NaCl aqueous solution was added. Dichloromethane was extracted to obtain a dichloromethane layer, which was evaporated to dry to obtain an extract rich in alkaloid extract. The extract of alkaloid extract has undergone repeated silica gel chromatography, Sephadex LH-20, ODS and liquid phase separation methods, combined with physical and chemical properties and modern spectroscopy methods (UV, IR, MS, 1H-NMR, 13C-NMR and 2D- NMR) isolated and identified two monomeric compounds, namely purslane amide B and orange piperonamide.

Embodiment 2

[0020] Example 2: Study on the antitumor activity of purslane amide B and orange piperonamide in vitro.

[0021] The inhibitory activities of purslane amide B and orange piperonamide on human lung adenocarcinoma cells (A549) and human leukemia cell lines (K562) were determined by MTT method.

[0022] (1) Instruments and reagents: RPMI 1640 culture medium and DMEM medium were purchased from Hyclone Company; fetal bovine serum was from Hangzhou Sijiqing; CKX31 inverted microscope (Olympus, Philippines); constant temperature CO2 incubator (Thermo, USA) ); 5810 R desktop high-speed low-temperature centrifuge (Eppendorf, Germany); automatic microplate reader (Biotek Group, USA).

[0023] (2) Cell culture: A549 cells were cultured in DMEM medium (containing 10% fetal bovine serum), K562 were cultured in 1640 medium (containing 10% fetal bovine serum), 37°C, containing 5% CO 2 cultured in a humidified incubator. Passage every 2-3 days. During the experiment, the cells in the logar...

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Abstract

The invention discloses an application of a purslane alkaloid monomeric compound in the preparation of an anti-tumor medicament, wherein the purslane alkaloid monomeric compound refers to purslane amide B and aurantiamide acetate, and inhibitory activities of the two monomeric compounds on human lung adenocarcinoma cells (A549) and human leukemia cell lines (K562) are detected by using MTT (methyl thiazolyl tetrazolium) ([3-(4,5)-dimethyl-2-thiazole-(2,5)-phenyl tetrazolium bromide]. The results show that the two monomeric compounds have an obvious growth inhibition, thereby indicating that the two monomeric compounds of purslane amide B and aurantiamide acetate can be applied to the preparation of the anti-tumor medicament.

Description

technical field [0001] The invention relates to the application of a purslane alkaloid monomer compound in the preparation of antitumor drugs, which belongs to the field of medicine. Background technique [0002] Cancer is a formidable enemy that endangers human health. There are about 1.6 million new cancer cases in my country every year, and about 1.3 million people die from cancer every year. The number of deaths due to cancer in the country is increasing year by year. my country is a high-incidence area for lung cancer, breast cancer, liver cancer and other cancers. Cancer mortality Go stay high. [0003] In the past half a century, a large number of scientific research forces have devoted themselves to the research of anti-tumor drugs, and some effective drugs have been launched to benefit cancer patients, but there are still many problems to be solved, such as the selective inhibition of tumor cells. Poor, some antineoplastic drugs trigger the formation of new tumors...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K31/7056A61K31/166A61P35/00A61P35/02
Inventor 赵庆春宋少江丁怀伟史国兵
Owner GENERAL HOSPITAL OF THE NORTHERN WAR ZONE OF THE CHINESE PEOPLES LIBERATION ARMY
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