Aseptic seeding and rapid tissue-culture propagation method of Callicarpa nudiflora Hook.ex Am

A technique of nudibranch and sterile seeding, which is applied in horticultural methods, botanical equipment and methods, horticulture, etc., can solve the problems of low seedling emergence rate, slow propagation speed, and small cutting propagation coefficient, etc., and achieve high reproduction rate , the effect of simple operation

Inactive Publication Date: 2013-06-05
海南省农业科学院园林花卉研究所
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Problems solved by technology

[0003] The object of the present invention is to provide a method for aseptic sowing and rapid tissue culture propagation of A. nudiflora. The method is simple to operate and has a high reproduction rate. It can quick

Method used

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Examples

Experimental program
Comparison scheme
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Example Embodiment

[0013] 1 Materials and methods

[0014] 1.1 Disinfection of materials and explants

[0015] The material is the mature seeds of P. nudiflora collected from Wuzhi Mountain in Hainan. Wash the naked flower purple bead seeds in tap water and wrap them with gauze, soak them in 75% alcohol for 10 s, rinse them with sterile water for 3 times, and then use 0.1% HgCl on a clean bench. 2 Soak the solution for 10 minutes, rinse with sterile water 5 times, and then inoculate the seeds on the induction medium.

[0016] 1.2 Medium

[0017] Using MS as the basic medium, add different concentrations of plant growth regulators 6-BA (6-benzyl adenine) and NAA (naphthalene acetic acid) according to the test purpose, plus 30 g / L sucrose and 6 g / L carrageenan, The pH is 5.8, and after preparing the aliquots, sterilize at a temperature of 121°C and a pressure of 0.14 MPa for 20 min.

[0018] 1.3 Proliferation of cluster buds

[0019] 1.3.1 The effect of growth regulator combination on the proliferation of ...

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Abstract

The invention discloses an aseptic seeding and rapid tissue-culture propagation method of Callicarpa nudiflora Hook.ex Am. The aseptic seeding and rapid tissue-culture propagation method comprises the following steps of: selecting mature seeds of Callicarpa nudiflora Hook.ex Am, performing primary culture on the mature seeds to form adventitious buds, and carrying out subculture multiplication and rooting culture on the adventitious buds. The tissue culture method is used for propagating the Callicarpa nudiflora Hook.ex Am, is simple in operation and high in propagation rate, is capable of rapidly and largely producing the Callicarpa nudiflora Hook.ex Am tissue culture seedlings with consistent characters, and solves the problems of low seeding emergence rate and too low propagation speed due to a small cutting propagation coefficient. The aseptic seeding and rapid tissue-culture propagation method can be used in industrialized production, provides an effective new path for protection, development and utilization of the Callicarpa nudiflora Hook.ex Am, and has important economic, social and biological benefits.

Description

technical field [0001] The invention relates to a method for aseptic seeding and rapid tissue culture propagation of Auranthus nudiflora. Background technique [0002] Callicarpa nudiflora Hook.ex Am is a small perennial shrub of the Verbenaceae family. It is mainly produced in Hainan, China, and also distributed in Guangdong and Guangxi. It is mainly distributed in Vietnam, Malaysia and India abroad. Naked flower purple pearl is a precious medicinal material, which was first recorded in "Supplement to Materia Medica" in Tang Dynasty. Its roots, stems, and leaves can be used as medicine, which has the effects of anti-inflammatory, astringent, hemostasis, heat-clearing and detoxification. Nudehuazizhu has a wide range of uses. It has a long history of medicinal use, wide range of effects and significant curative effects. At present, there are nudehuazizhu capsules, nudehuazizhu tablets, nudehuazizhu suppositories and other finished medicines in clinical medicine. In addition,...

Claims

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Application Information

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IPC IPC(8): A01H4/00
Inventor 潘梅姜殿强云勇黄赛王景飞吕德任戚华莎
Owner 海南省农业科学院园林花卉研究所
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