Aseptic seeding and rapid tissue-culture propagation method of Callicarpa nudiflora Hook.ex Am

Abstract

The invention discloses an aseptic seeding and rapid tissue-culture propagation method of Callicarpa nudiflora Hook.ex Am. The aseptic seeding and rapid tissue-culture propagation method comprises the following steps of: selecting mature seeds of Callicarpa nudiflora Hook.ex Am, performing primary culture on the mature seeds to form adventitious buds, and carrying out subculture multiplication and rooting culture on the adventitious buds. The tissue culture method is used for propagating the Callicarpa nudiflora Hook.ex Am, is simple in operation and high in propagation rate, is capable of rapidly and largely producing the Callicarpa nudiflora Hook.ex Am tissue culture seedlings with consistent characters, and solves the problems of low seeding emergence rate and too low propagation speed due to a small cutting propagation coefficient. The aseptic seeding and rapid tissue-culture propagation method can be used in industrialized production, provides an effective new path for protection, development and utilization of the Callicarpa nudiflora Hook.ex Am, and has important economic, social and biological benefits.

Description

technical field [0001] The invention relates to a method for aseptic seeding and rapid tissue culture propagation of Auranthus nudiflora. Background technique [0002] Callicarpa nudiflora Hook.ex Am is a small perennial shrub of the Verbenaceae family. It is mainly produced in Hainan, China, and also distributed in Guangdong and Guangxi. It is mainly distributed in Vietnam, Malaysia and India abroad. Naked flower purple pearl is a precious medicinal material, which was first recorded in "Supplement to Materia Medica" in Tang Dynasty. Its roots, stems, and leaves can be used as medicine, which has the effects of anti-inflammatory, astringent, hemostasis, heat-clearing and detoxification. Nudehuazizhu has a wide range of uses. It has a long history of medicinal use, wide range of effects and significant curative effects. At present, there are nudehuazizhu capsules, nudehuazizhu tablets, nudehuazizhu suppositories and other finished medicines in clinical medicine. In addition,...

AI Technical Summary

Problems solved by technology

[0003] The object of the present invention is to provide a method for aseptic sowing and rapid tissue culture propagation of A. nudiflora. The method is simple to operate and has a high reproduction rate. It can quickly mass-produce A. nudiflora tissue culture seedlings with uniform properties, and solves the problem of seedling emergence rate. Low, the cutting propagation coefficient is small and the propagation speed is too slow