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Method of extracting polysaccharide from pine pollen

An extraction method and a crude polysaccharide technology, applied in the field of crude polysaccharide extraction, can solve the problems of low yield and purity, unsuitable for large-scale promotion in production enterprises, energy consumption in safety accidents, and the like, achieving simple process, saving organic solvents, and reducing risks Effect

Active Publication Date: 2013-06-05
YANTAI NEW ERA HEALTH IND +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] The extraction process of polysaccharides mostly adopts methods such as hot water extraction, dilute salt, dilute alkali solution extraction, enzymatic hydrolysis extraction, etc., and most of the products obtained have low yield and purity
In addition, the traditional extraction process often uses explosive chemicals such as ethanol and chloroform, which is prone to safety accidents and consumes a lot of energy, which is extremely unsuitable for large-scale promotion by production enterprises

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0044] Take 5kg of broken wall pine pollen, put it into ultrasonic extraction equipment according to the ratio of broken wall pollen:water of 1:6, the water temperature is 55°C, the ultrasonic frequency is 69.4KHz, and the stirring speed is 15rpm. After 0.5 hours, the filtrate was collected by centrifugation at 5000 rpm, and processed through 1 μm and 0.2 μm plate-and-frame filter equipment in sequence. A total of two extractions were performed, and the filtrates were combined. The obtained liquid is treated by ultrafiltration membrane filtration equipment, the molecular weight of separation is 5000Da, the operating temperature is controlled at 25°C, and the liquid with molecular weight > 5000Da is collected. Add TCA solid to the extraction liquid until the mass ratio of the extraction liquid to TCA is 0.97:0.03, stir well for 20 minutes, let stand at a low temperature of 4°C overnight, centrifuge at 5000rpm for 0.5h, remove the protein precipitate, and the obtained liquid is ...

Embodiment 2

[0046] Take 5kg of broken-walled pine pollen, put it into ultrasonic extraction equipment according to the ratio of broken-walled pollen:water of 1:10, water temperature: 60°C, ultrasonic frequency is 72KHz, stirring speed is 18rpm, and after 0.5 hours, the filtrate is collected by centrifugation at 5000rpm. The filtrate is processed through 1 μm and 0.2 μm plate and frame filter equipment in sequence. A total of two extractions were performed, and the filtrates were combined. The obtained clarified filtrate is treated by ultrafiltration membrane filtration equipment, the molecular weight of separation is 5000Da, the operating temperature is 25°C, and the liquid with molecular weight > 5000Da is collected. Add solid TCA to the obtained liquid until the mass ratio of the extract to TCA is 0.95:0.05, stir well for 20 minutes, let stand overnight at 4°C, and centrifuge at 5000rpm for 0.5h to remove protein precipitates. The obtained liquid is vacuum concentrated and vacuum freeze...

Embodiment 3

[0048] Take 5kg of broken-walled pine pollen, put it into ultrasonic extraction equipment according to the ratio of broken-walled pollen:water of 1:8, water temperature: 50°C, ultrasonic frequency is 67KHz, stirring speed is 16rpm, after 35 minutes, centrifuge at 7000rpm to collect the filtrate. The filtrate is processed through 1 μm and 0.2 μm plate and frame filter equipment in sequence. A total of two extractions were performed, and the filtrates were combined. The obtained clarified filtrate is treated by ultrafiltration membrane filtration equipment, the molecular weight of separation is 7000Da, the operating temperature is 35°C, and the liquid with molecular weight > 7000Da is collected. Add solid TCA to the obtained liquid until the mass ratio of the extract to TCA is 0.96:0.04, stir thoroughly for 20 minutes, let stand at a low temperature of 4°C overnight, and centrifuge at 7000rpm for 0.5h to remove protein precipitates. The obtained liquid is vacuum concentrated and...

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PUM

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Abstract

The invention relates to a method for extracting polysaccharide from pine pollen. The method for extracting the polysaccharide from the pine pollen is characterized by comprising the following steps. Firstly, wall-breaking treatment is performed on the pine pollen, and the pine pollen is obtained. Secondly, the treatment of ultrasound and hot water extraction is performed on wall-breaking pollen, stirring is performed, the treatment of centrifugation is performed, filtering is performed and then supernate is obtained. Thirdly, the supernate is processed by an ultrafiltration membrane filtration system, and collected fluid is collected. Fourthly, trichloroacetic acid is added in the collected liquid, the treatment of centrifugation is performed, fibronectin in the collected liquid is removed, and a polysaccharide solution is obtained. Fifthly, vacuum concentration is performed on the polysaccharide solution, and a polysaccharide concentrated solution is obtained. Sixthly, the treatment of vacuum freeze drying is performed on the polysaccharide concentrated solution, and the polysaccharide is obtained. The method for extracting the polysaccharide from the pine pollen is simple in technology, strong in operability, capable of saving a large quantity of organic solvents, explosive compounds of ethanol, chloroform, n-butyl alcohol and the like are not needed, and capable of reducing the risks in production of products.

Description

technical field [0001] The invention relates to a method for extracting crude polysaccharide from pine pollen. Background technique [0002] Pine pollen is the pollen of Pinus massoniana, Pinus tabulaeformis or plants of the same genus. It is a variety of both medicine and food in the treasure house of Chinese medicine. As a traditional Chinese medicinal material, its history of both medicine and food has exceeded thousands of years. It is mainly distributed in Zhejiang and Jiangsu of my country. , Hebei, Liaoning, Jilin, Hubei and other places. Dried pine pollen is a light yellow fine powder, in the form of uniform small round grains, with a light body, easy to fly, smooth, not sinking in water, slightly fragrant, and greasy. Pine pollen contains a variety of nutrients, including proteins, amino acids, monosaccharides, polysaccharides, nucleic acids, minerals and other active ingredients. Modern scientific research has found that pine pollen has various functional and acti...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C08B37/00
Inventor 刘志河耿越王桐钤莉妍杨长军
Owner YANTAI NEW ERA HEALTH IND