Bacteria compsite inoculum used in lignin decomposition

A technology of composite bacterial agent and lignin, applied in the field of microorganisms

Active Publication Date: 2013-06-05
HEILONGJIANG BAYI AGRICULTURAL UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, there are relatively few studies on the decomposition of lignin by bacteria, and the research on the decomposition of lignin by bacteria is mostly focused on exploring new bacteria and new lignin decomposition pathways. Research is even less reported

Method used

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Examples

Experimental program
Comparison scheme
Effect test

specific Embodiment approach 1

[0008] Specific embodiment one: The bacterial composite bacterial agent for the decomposition of lignin of the present embodiment comprises 25 parts of pseudomonas ( Pseudomonas sp.) ACCC 11691 fermentation broth, 15 parts of Pseudomonas schinii ( Pseudomonas stutzeri ) DSM 6084 fermentation broth, 25 microbacteria ( Microbacterium sp.) DSM 20618 fermentation broth, 20 parts Acinetobacter ( Acinetobacter genospecies 3) DSM 9318 fermentation broth; the number of effective viable bacteria in the fermentation broth of the above four bacteria is 10 9 Individual / mL or more.

[0009] The steps of the preparation method of this composite bacterial agent are as follows:

[0010] (1) Activation of strains: Inoculate the preserved Pseudomonas ACCC 11691, Pseudomonas schwartz DSM 6084, Microbacterium DSM 20618 and Acinetobacter DSM 9318 into 100mL Erlenmeyer flasks containing 50mL activation medium Inside, the inoculation amount is 5%~10%, the culture temperature is 25°C~30°C, an...

specific Embodiment approach 2

[0017] Specific embodiment two: The bacterial composite microbial agent for lignin decomposition of the present embodiment comprises 30 parts of Pseudomonas ACCC 11691 fermentation broth, 25 parts of Pseudomonas shiitake DSM 6084 fermentation broth, 35 parts by weight. Part Microbacterium DSM 20618 fermentation broth, 25 parts Acinetobacter DSM 9318 fermentation broth; the number of effective viable bacteria in the fermentation broth of the above four kinds of bacteria is 10 9 Individual / mL or more. The preparation method of the composite bacterial agent of this embodiment is the same as that of Embodiment 1.

[0018] The composite bacterial agent of this embodiment is inoculated in the MSM medium with 1 g / L alkaline lignin as the carbon source, the initial pH value is 8.0, and the decomposition rate of alkaline lignin can reach 20.1 %. The composite bacterial agent was inoculated in MSM medium with 2% reed as carbon source, the initial pH value was 8.0, and it was cultured ...

specific Embodiment approach 3

[0020] Specific embodiment three: The bacterial composite microbial agent for lignin decomposition of the present embodiment comprises 26 parts of Pseudomonas ACCC 11691 fermentation broth, 16 parts of Pseudomonas shiitake DSM 6084 fermentation broth, 26 parts by weight. Part Microbacterium DSM 20618 fermentation liquid, 21 parts Acinetobacter DSM 9318 fermentation liquid; The effective number of viable bacteria in the fermentation liquid of above four kinds of bacteria is 10 9 Individual / mL or more. The preparation method of the composite bacterial agent of this embodiment is the same as that of Embodiment 1.

[0021] The composite bacterial agent of this embodiment is inoculated in MSM medium with 1 g / L alkaline lignin as the carbon source, the initial pH value is 8.0, and the decomposition rate of alkaline lignin can reach 20.2 %. The composite bacterial agent was inoculated in the MSM medium with 2% reed as carbon source, the initial pH value was 8.0, and the static cult...

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Abstract

The invention relates to a bacteria composite inoculum used in lignin decomposition. The invention belongs to the technical field of microorganism. With the inoculum, a current problem of a lack of lignin decomposition bacterial composite strain is solved. The composite inoculum comprises, by weight, 25-30 parts of pseudomonas ACCC11691 fermentation broth, 15-25 parts of pseudomonas stutzeri DSM6084 fermentation broth, 25-35 parts of microbacterium DSM20618 fermentation broth, and 20-25 parts of acinetobacter DSM9318 fermentation broth. Effective viable cell numbers in the four bacteria fermentation broths are all higher than 10<9> / mL. The composite inoculum provided by the invention provides a substantial decomposition effect upon lignin. The invention provides an application basis for papermaking pulping processes with cellulose as a main raw material, and provides a research basis for bacterial decomposition of lignin.

Description

technical field [0001] The invention belongs to the field of microorganisms, and in particular relates to a composite microbial agent composed of various microbial fermentation liquids. Background technique [0002] Lignin is a macromolecular compound with a complex structure widely present in plants. It forms a supramolecular structure together with cellulose and hemicellulose, and plays a role in enhancing the mechanical strength of plant cells. Reed is a kind of gramineous herb, which is widely distributed in the Liaohe Delta, Songnen Plain and Sanjiang Plain in the northeast. According to statistics, the total output of reed in the above areas is about 3 million tons per year. Because of its higher fiber content than other herbaceous plants, reed , with longer fiber length, has always been the preferred raw material for papermaking in herbaceous plants. The main component of reed straw is lignocellulose. Lignin is one of the components of lignocellulose, but it is also ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/00C12N1/20D21C3/00C12R1/38C12R1/01
Inventor 王伟东王彦杰王艳霞晏磊高亚梅刘权
Owner HEILONGJIANG BAYI AGRICULTURAL UNIVERSITY
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