Bacteria compsite inoculum used in lignin decomposition
A technology of composite bacterial agent and lignin, applied in the field of microorganisms
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specific Embodiment approach 1
[0008] Specific embodiment one: The bacterial composite bacterial agent for the decomposition of lignin of the present embodiment comprises 25 parts of pseudomonas ( Pseudomonas sp.) ACCC 11691 fermentation broth, 15 parts of Pseudomonas schinii ( Pseudomonas stutzeri ) DSM 6084 fermentation broth, 25 microbacteria ( Microbacterium sp.) DSM 20618 fermentation broth, 20 parts Acinetobacter ( Acinetobacter genospecies 3) DSM 9318 fermentation broth; the number of effective viable bacteria in the fermentation broth of the above four bacteria is 10 9 Individual / mL or more.
[0009] The steps of the preparation method of this composite bacterial agent are as follows:
[0010] (1) Activation of strains: Inoculate the preserved Pseudomonas ACCC 11691, Pseudomonas schwartz DSM 6084, Microbacterium DSM 20618 and Acinetobacter DSM 9318 into 100mL Erlenmeyer flasks containing 50mL activation medium Inside, the inoculation amount is 5%~10%, the culture temperature is 25°C~30°C, an...
specific Embodiment approach 2
[0017] Specific embodiment two: The bacterial composite microbial agent for lignin decomposition of the present embodiment comprises 30 parts of Pseudomonas ACCC 11691 fermentation broth, 25 parts of Pseudomonas shiitake DSM 6084 fermentation broth, 35 parts by weight. Part Microbacterium DSM 20618 fermentation broth, 25 parts Acinetobacter DSM 9318 fermentation broth; the number of effective viable bacteria in the fermentation broth of the above four kinds of bacteria is 10 9 Individual / mL or more. The preparation method of the composite bacterial agent of this embodiment is the same as that of Embodiment 1.
[0018] The composite bacterial agent of this embodiment is inoculated in the MSM medium with 1 g / L alkaline lignin as the carbon source, the initial pH value is 8.0, and the decomposition rate of alkaline lignin can reach 20.1 %. The composite bacterial agent was inoculated in MSM medium with 2% reed as carbon source, the initial pH value was 8.0, and it was cultured ...
specific Embodiment approach 3
[0020] Specific embodiment three: The bacterial composite microbial agent for lignin decomposition of the present embodiment comprises 26 parts of Pseudomonas ACCC 11691 fermentation broth, 16 parts of Pseudomonas shiitake DSM 6084 fermentation broth, 26 parts by weight. Part Microbacterium DSM 20618 fermentation liquid, 21 parts Acinetobacter DSM 9318 fermentation liquid; The effective number of viable bacteria in the fermentation liquid of above four kinds of bacteria is 10 9 Individual / mL or more. The preparation method of the composite bacterial agent of this embodiment is the same as that of Embodiment 1.
[0021] The composite bacterial agent of this embodiment is inoculated in MSM medium with 1 g / L alkaline lignin as the carbon source, the initial pH value is 8.0, and the decomposition rate of alkaline lignin can reach 20.2 %. The composite bacterial agent was inoculated in the MSM medium with 2% reed as carbon source, the initial pH value was 8.0, and the static cult...
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