Vascular endothelial cell elastic substrate culture device

Abstract

The invention relates to a vascular endothelial cell elastic substrate culture device. The interior of an elastic substrate culture cavity is connected with an elastic substrate through a group of rolling wheels, a driving shaft of the rolling wheels is connected with a stepping motor, a CO2 inlet and outlet and a transparent observation window are arranged on a bottom plate on the elastic substrate culture cavity, a liquid inlet of the elastic substrate culture cavity is connected with a heart pump through an energy storage device, the liquid inlet of the heart pump is connected with a liquid storage groove and a constant-temperature device, the elastic substrate culture cavity is connected to the whole device through a liquid inlet and outlet, and a first peripheral resistance device and a second peripheral resistance device are respectively connected in a main passage and a pulsating diversion passage. The heart pump can simulate the intermittent ejection function and respectively provide pulsating flows for the pulsating diversion passage and the main passage. Under the conditions of the pulsating flows, an in-vitro environment with appropriate temperature, PH value and stable CO2 concentration can be provided for growth of endothelial cells, the simultaneous presence of normal stress, shear stress and tensile stress can be realized, and the dynamic culture of the endothelial cells can be mutually and independently regulated.

Description

technical field [0001] The invention relates to a vascular endothelial cell culture device, in particular to an elastic substrate culture chamber which can provide adjustable tensile stress for endothelial cells, and can realize dynamic culture of endothelial cells with relatively independent regulation of normal stress, shear stress and tensile stress. Background technique [0002] Vascular endothelial cells are one of the largest tissues with secretory function in the human body and participate in the normal regulation of the body. Structural variation and dysfunction of endothelial cells can easily induce a series of vascular-related diseases such as atherosclerosis. However, in vitro endothelial cells can be used as experimental materials for the study of these diseases. Therefore, under pulsating flow conditions, it is very important to provide a dynamic culture device for vascular endothelial cells that is closer to the hemodynamic environment of the human body. The h...

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Problems solved by technology

[0003] At present, it is difficult for the endothelial cell culture device to achieve relatively independent adjustment of the tensile stress of endothelial cells. At the same time, it is difficult to solve the contradiction between the culture substrate required for endothelial cell growth and the simulation degree of human blood vessels, that is, the elastic circular tube is closer to the human blood vessel, but the in vitro circular tube environment It is not easy to plant and grow endothelial cells and it is inconvenient for experimental observation, while glass slides or cell culture plates are convenient for planting and growing endothelial cells, but they are quite different from the characteristics of human blood vessels

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