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Method for improving biogas yield of lignocelluloses-containing raw material

A technology of lignocellulose and cellulase, applied in the field of enzyme engineering, can solve the problems of low gas production, waste, no steam production, etc., and achieve the effect of increasing biogas production, improving utilization rate, and simple operation

Inactive Publication Date: 2015-04-29
SHANDONG UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, there are some common problems in the actual use of biogas digesters, which are manifested as the raw materials for biogas fermentation are not fully utilized and are wasted, and the actual biogas conversion rate (raw material utilization rate) is very low
Especially when the fermentation raw material contains lignocellulose, the digestibility of the raw material is often very low, and the raw material is easy to float on the liquid surface, and is not easy to be used by microorganisms. Low gas volume or even no steam production

Method used

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  • Method for improving biogas yield of lignocelluloses-containing raw material
  • Method for improving biogas yield of lignocelluloses-containing raw material

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0034] A method for improving biogas production containing lignocellulosic raw materials, characterized in that the steps are as follows:

[0035] (1) Take 30kg of lignocellulose-containing raw materials, add cellulosic enzyme solution in an amount of 3mg / g, and add 5 times the weight of water to the lignocellulose-containing raw materials on a dry weight basis, and treat at 35°C for 1 hour, Prepare pretreated raw materials;

[0036] (2) Anaerobic fermentation of the pretreated raw material obtained in step (1) in a biogas digester to obtain biogas. For the steps and conditions of anaerobic fermentation in the biogas digester, please refer to the content recorded in the "Comparative Test of Biogas Production by Different Pretreatments of Corn Stalks" published by Huang Xiao et al. in "Agricultural Engineering Technology (New Energy Industry)" Issue 02, 2010.

[0037] The preparation method of the cellulase liquid in the step (1) is as follows:

[0038] Take the cellulase-pro...

experiment example 1

[0044] Aspergillus aculeatus (Aspergillus aculeatus), Trichoderma reesei (Trichoderma reesei), and Penicillum decumbens (Penicillum decumbens) were selected as cellulase-producing strains and labeled as experimental group 1, experimental group 2, and experimental group 3, respectively. The method described in Example 1 was used to prepare biogas, and at the same time, raw materials not pretreated with cellulase liquid were used as control group 1. The experimental results are shown in Table 1.

[0045] Table 1

[0046]

[0047] Result analysis:

[0048] It can be clearly seen from the above table that the cumulative gas production and raw material gas production of the experimental group 1 to 3 in the first 18 days were higher than those of the control group 1, and the total gas production of the experimental group 2 was increased by 3.8 times, and the raw material gas production was also higher than that of the control group 1. Increased by as much as 5.4 times, it can be s...

Embodiment 2

[0050] A method for improving biogas production containing lignocellulosic raw materials, characterized in that the steps are as follows:

[0051] (1) Take 30kg of lignocellulose-containing raw materials, add cellulosic enzyme solution in an amount of 10mg / g, and add 10 times the weight of water to the lignocellulose-containing raw materials on a dry weight basis, and treat at 45°C for 6 hours, Prepare pretreated raw materials;

[0052] (2) Anaerobic fermentation of the pretreated raw material obtained in step (1) in a biogas digester to obtain biogas. For the steps and conditions of anaerobic fermentation in the biogas digester, please refer to the content recorded in the "Comparative Test of Biogas Production by Different Pretreatments of Corn Stalks" published by Huang Xiao et al. in "Agricultural Engineering Technology (New Energy Industry)" Issue 02, 2010.

[0053] The preparation method of the cellulase liquid in the step (1) is as follows:

[0054] Take the cellulase-...

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Abstract

The invention relates to a method for improving a biogas yield of a lignocelluloses-containing raw material. The method comprises the following steps: (1), getting the lignocelluloses-containing raw material, adding cellulase liquid, water or biogas slurry, and treating for 1-48 hours at 35 DEG C to 50 DEG C to obtain the pre-treated raw material; and (2), carrying out anaerobic fermentation onto the pre-treated raw material obtained in the step (1) through a biogas digester to obtain the biogas. According to the method for improving the biogas yield of the lignocelluloses-containing raw material disclosed by the invention, pre-treatment is carried out onto the lignocelluloses-containing biogas digester raw material by the cellulase liquid, so that degradation-resistant cellulose substances in the raw material are preliminarily degraded by the cellulase, and therefore, the substrate utilization rate of biogas-producing microorganisms in the anaerobic fermentation is improved, the biogas yield of the biogas digester is improved, and the problem that the raw materials are floated on the biogas slurry and difficult to degrade after the raw materials are added to the biogas digester is solved.

Description

technical field [0001] The invention relates to a method for increasing the yield of biogas containing lignocellulose raw materials, which belongs to the technical field of enzyme engineering. Background technique [0002] In recent years, biogas as a renewable green and clean energy has attracted more and more attention. Especially in rural areas, a large number of biomass resources and secondary metabolites in the farming industry cannot be well utilized. Biomass and metabolites are fermented to produce biogas, which is a very good way to use, and now this clean energy reproduction technology has already spread to the vast rural areas. In addition, the produced biogas can be further processed to utilize CO and H 2 Transformed into a substitute for non-renewable resource petroleum. However, there are some common problems in the actual use of biogas digesters, such as the fact that the raw materials for biogas fermentation are not fully utilized and are wasted, and the ac...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12P5/02C12R1/80C12R1/66C12R1/885C12R1/645
CPCY02E50/30
Inventor 方诩侯少莉韩丽娟王明钰刘凯丽刘诗佳
Owner SHANDONG UNIV