Method for predicting height of excellent ice-snow athlete by using polymorphism of COL11A1 gene promoter

A molecular biology and athlete technology, applied in the interdisciplinary field of sports science and molecular biology, can solve problems such as interfering with transcriptional protein binding and affecting mRNA expression, and achieve the effect of simple and easy operation.

Inactive Publication Date: 2013-06-19
HARBIN INST OF PHYSICAL EDUCATION
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AI Technical Summary

Problems solved by technology

[0007] rs6692914 is located in the promoter region of the COL11A1 gene, and the variation in this region may interfere with the binding of transcriptional proteins, thereby affecting the expression of mRNA

Method used

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Embodiment Construction

[0009] (1) Extraction of peripheral blood DNA

[0010] Take 3~5ml of cubital venous blood from individual and place in EDTA-Na 2 Store in anticoagulation tube at -80℃ for later use. Using QIAamp DNA Blood MidiKit (Qiagen Company, Germany) kit, the whole genome DNA of the research object was extracted according to the instructions. The DNA concentration was measured with Nanodrop full-wavelength spectrophotometer (ND-1000, USA) and standardized to 50ng / μl.

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Abstract

The invention discloses a molecular biology method for predicting height of an excellent ice-snow athlete. The method comprises the following steps: detecting the genetic polymorphism of a COL11A1 gene promoter by acquisition of peripheral blood of an excellent ice-snow athlete and a common person, DNA (deoxyribonucleic acid) extraction, genotyping, height standardized transformation and statistic analysis; and predicting the height of the excellent ice-snow athlete by using the polymorphism site of the COL11A1 gene promoter rs6692914. The invention lays firm theoretical and technological foundation for establishing a scientific system of potential early prediction, assessment and talent selection and cultivation of the ice-snow athlete, enhances the success rate of talent selection, and breaks through the theoretical and technical bottleneck in one-time talent selection.

Description

Technical field [0001] The invention relates to a molecular biology method for predicting the height of outstanding ice and snow athletes. It is suitable for the early selection of outstanding ice and snow athletes at the genetic level, and belongs to the interdisciplinary field of sports science and molecular biology. Background technique [0002] With the rapid development and scientific process of modern competitive sports training, more and more attention is paid to the influence of athlete's physical characteristics on sports performance. Although athletes possessing ideal physical conditions are not the only prerequisite for exerting their competitive potential and improving sports performance, it is a starting point for athletes to receive competitive sports training. As one of the important factors for predicting the human body's maximum exercise potential, body shape characteristics should not be underestimated in competitive sports training. Comprehensive consideratio...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/68
Inventor 阚军常汪宇峰李嘉孙博孙玉娇关伟军
Owner HARBIN INST OF PHYSICAL EDUCATION
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