Preparation technology for improving Fuzhuan tea pollen germination rate by using inoculation method

The invention relates to a technology of tea bloom rate and preparation process, which is applied in the field of preparation technology for improving the bloom rate of Fu brick tea by inoculation method, and can solve the problems of low bloom rate and the like.

Inactive Publication Date: 2013-06-26
YIYANG TEA FACTORY +1
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

In the past, the research on the flowering technology of Fuzhuan brick tea only stayed in the process of controlling the temperature and humidity in the

Method used

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  • Preparation technology for improving Fuzhuan tea pollen germination rate by using inoculation method
  • Preparation technology for improving Fuzhuan tea pollen germination rate by using inoculation method
  • Preparation technology for improving Fuzhuan tea pollen germination rate by using inoculation method

Examples

Experimental program
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Effect test

Embodiment 1

[0045] Embodiment 1: Separation and purification of Flos Chinensis:

[0046] The finished Xiangyi brand fu brick sample was pulverized with a plant micro pulverizer, sieved with 200 mesh, dried at 65°C for 2 hours, weighed 1g sample, fixed volume 10mL, and prepared 10 -1 、10 -2 、10 -3 、10 -4 , 10 -5 Each 10mL, take 10 -3 , 10 -4 , 10 -5 Apply 0.5mL of each of the three gradients to the PDA agar solid medium plate added with 10% Fuzhuan tea juice, blow dry on the ultra-clean table, and culture at 28°C for 3 days, pick the golden yellow dominant colony, and draw the line until the addition of 10% Fuzhuan tea On the PDA plate of the brick tea juice, the culture was continued under the same conditions until purification.

[0047] The isolated and purified golden flower colonies are shown in the appendix figure 1.

Embodiment 2

[0048] Embodiment 2: the spore suspension preparation of golden flower:

[0049] Pick the purified golden flower colony, inoculate it into the PDA liquid medium supplemented with 10% Fuzhuan tea juice, 20% sucrose and 10% NaCl, and culture at 28°C for 2 days, then raise the temperature to 30-32°C for cultivation 12-28 days, shake to disperse the conidia, filter with double sterile gauze, then 280rpm shake to cultivate and disperse the conidia, dilute the spore suspension with sterile water until the OD value is 0.6, which is the usable golden flower spore suspension liquid.

[0050] Raise the temperature to 30-32°C and cultivate for 12-28 days, for example: raise the temperature to 30°C and cultivate for 28 days; or raise the temperature to 32°C and cultivate for 12 days; or raise the temperature to 31°C and cultivate for 20 days sky.

[0051] The results of the spore suspension culture of Jinhua are attached figure 2 .

Embodiment 3

[0052] Example 3: Verification of the improvement of the flowering rate of black hair tea from Yiyang Tea Factory's best-grade Fu bricks, first-grade Fu bricks, and common people's Fu bricks

[0053] Take respectively 20Kg of the black hair tea of ​​Yiyang Tea Factory's top grade Fuzhuan, Yipin Fuzhuan and Minmin Fuzhuan, and use no inoculation as a contrast, and carry out flowering experiments in Yiyang Tea Factory according to steps 1-7 in the specific embodiment (wherein 3rd, Steps 5, 6, and 7 are the treatment methods of Yiyang tea factory), and the flowering rate of the three kinds of Fu bricks can reach 100%.

[0054] See attached image 3 . In the picture, the top-quality Fuzhuan Fahua is on the left, the first-grade Fuzhuan Fahua is in the middle, and the Fuzhuan Fahua is on the right.

[0055] For its flowering efficiency statistics, see Figure 4 verification form.

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Abstract

The invention relates to a preparation technology for improving Fuzhuan tea pollen germination rate by using an inoculation method. The preparation technology comprises the following specific steps of: (1) purification and separation of eurotium cristatum; (2) preparation of eurotium cristatum spore suspension with certain aging degree and determination of concentration; (3) treatment on a raw dark green tea sample; (4) adding the eurotium cristatum spore suspension; (5) pressing; (6) pollen germination; and (7) drying and storage. The preparation technology develops preparation technology conditions for improving the Fuzhuan tea pollen germination rate by using the inoculation method, and can be used for realizing the pollen germination of Fuzhuan tea in a short term of 7-15 days. Moreover, golden flowers are generally blooming, the color is bright and golden, particles are plump, and the Fuzhuan tea pollen germination rate can reach 100% by using different raw dark green tea raw materials.

Description

technical field [0001] The invention relates to the technical field of agricultural product processing, in particular to a preparation process for improving the flowering rate of Fuzhuan brick tea by inoculation method, and the flower described in the flowering is Saccharomyces coronata ( Eurotium cristarum (Raper & Fennell) Malloch & Cain), commonly known as golden flower. Background technique [0002] Fu brick tea is an indispensable and important drink for ethnic minorities in the frontier. The golden flowers are generally flourishing, the color is bright and golden, and the granules are hypertrophic, which has always been the most important quality characteristic of high-quality Fuzhuan tea. The golden flower is formed during the flowering stage during the processing of Fuzhuan tea. In the past, the research on flowering technology of Fuzhuan brick tea only stayed in the process of controlling temperature and humidity in the natural state, and the flowering rate was l...

Claims

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Application Information

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IPC IPC(8): A23F3/06
Inventor 刘石泉赵运林龙立平徐迪军刘杏益
Owner YIYANG TEA FACTORY
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