Device for high-efficient separation of magnetotactic bacteria in high gradient magnetic field

A high gradient magnetic field, magnetotactic bacteria technology, applied in the direction of stress-stimulated microbial growth and other directions, can solve the problems of low capture efficiency, induce and capture magnetotactic bacteria, affect the separation efficiency of magnetotactic bacteria, etc. Improved adsorption efficiency and no secondary pollution

Inactive Publication Date: 2013-07-03
XIAN JIAOTONG LIVERPOOL UNIV +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, some current methods using permanent magnets cannot efficiently induce and capture magnetotactic bacteria, such as the published Chinese patent application, publication number CN1693223A, which uses permanent magnets to induce and capture magnetotactic bacteria, and the capture efficiency is low. , so that many bacteria will be taken away by the solution, seriously affecting the separation efficiency of magnetotactic bacteria

Method used

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  • Device for high-efficient separation of magnetotactic bacteria in high gradient magnetic field
  • Device for high-efficient separation of magnetotactic bacteria in high gradient magnetic field

Examples

Experimental program
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Effect test

Embodiment 2

[0024] The inventive device is used to efficiently separate the magnetotactic bacteria from the solution.

[0025] Slowly inject 10 liters of the magnetotactic bacteria (Magnetospirillum sp.StrainAMB-I, purchased from ATCC, strain number: ATCC700264)) bacteria liquid to be treated into the prepared in Example 1 from the liquid inlet. In a container (length 500mm, diameter 100mm, copper wire turns 1000 turns). The power supply current (5 amps) was turned on so that the magnetic field on the axis of the container was about 0.01 Tesla. Under the capture of the high gradient magnetic field force near the steel wool, most of the magnetotactic bacteria will be adhered to the steel wool with a large specific surface area.

[0026] The treated solution was collected from the liquid outlet for analysis, and it was found that more than 98% of the magnetotactic bacteria were captured by the device, thereby achieving the purpose of efficiently separating the magnetotactic bacteria in the...

Embodiment 3

[0029] The inventive device is used to separate heavy metals from solution.

[0030] Add 100 mg of Ni2+, Cu2+, Pb2+, Cr2+ respectively to 10 liters of magnetotactic bacteria (Magnetospirillum sp.Strain AMB-I, purchased from ATCC, strain number: ATCC700264), Mixed, magnetotactic bacteria will combine heavy metal ions to become a solution of magnetotactic bacteria with heavy metal ions.

[0031] The solution was injected into the device according to the method of Example 2 for processing. Most of the magnetotactic bacteria that adsorb heavy metals will be captured by the steel wool.

[0032] The treated solution was collected from the liquid outlet, and the concentration of heavy metal ions was detected by atomic absorption spectroscopy. It was found that the removal efficiency of more than 92% could be achieved for different ions. Among them, Ni2+ is 94%, Cu2+ is 92.5%, Pb2+ is 92%, and Cr2+ is 93.5%.

[0033] After the power is turned off, since the magnetic field disappear...

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Abstract

The invention discloses a device for high-efficient separation of magnetotactic bacteria in a high gradient magnetic field, which comprises a container for accommodating liquid containing magnetotactic bacteria, and is characterized in that the container is disposed in a solenoid; the solenoid is connected with a low frequency power supply; a steel wool with magnetic conductibility is disposed in the container. The device of the invention has a high magnetic field gradient; the magnetic field force applied to the magnetotactic bacteria is hundred times higher than that applied by a permanent magnet, and thus the magnetotactic bacteria can be captured easily, and are not washed away with solutions. The adsorption efficiency is improved greatly. The high gradient magnetic field can be removed at any time by turning off the electromagnetic field, and thus the magnetotactic bacteria are easily washed off from the steel wool, realizing the purpose of separation or recovery. The device is low in technical cost, high in efficiency, and free of secondary pollution.

Description

technical field [0001] The invention belongs to the field of microorganism separation methods and sewage sludge treatment, and in particular relates to a device for efficiently separating magnetotactic bacteria under a high-gradient magnetic field. Background technique [0002] Magnetotactic bacteria are a class of microorganisms that can produce magnetosomes (ferric iron tetroxide) by themselves. This kind of bacteria can sense the earth's magnetic field or the external magnetic field to recognize the direction; at the same time, it can be induced by the external magnetic field. Based on this property, magnetotactic bacteria can be controlled, guided and captured by a magnetic field. The magnetotactic bacteria can be separated from the solution by adding an external magnetic field, which is a technology that can be widely used in microorganisms and other fields. In addition, studies have found that magnetotactic bacteria, as microorganisms, can bind certain metal particle...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12M1/42
Inventor 于昊张蓓蓓宋琴程国胜谢辰李梦狄徐光彩
Owner XIAN JIAOTONG LIVERPOOL UNIV
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