A kind of preparation method of microscale hydrogel
A hydrogel, micro-scale technology, applied in the field of biotechnology applications, can solve the problems of lengthy operation process, high production cost, expensive equipment, etc., and achieve the effects of high processing controllability, low cost, and high material utilization rate
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[0023] The preparation method of the micro-scale hydrogel of the present invention includes the following steps: 1) injecting the hydrogel precursor into the pen holder 2 of the sampling pen, and the sampling pen also includes a nib 1, and the front end of the nib 1 is provided with The sample outflow hole is provided with a rotatable ball 3 in the sample outflow hole. The rear end of the pen tip 1 is connected to the pen holder 2. After injecting the hydrogel precursor, seal the hydrogel precursor with sealing oil 6 (mineral oil). Sealed in the pen holder 2, the sample adding pen also includes a connection device 4 for connecting the pen head 1 and the pen holder 2, see figure 1 ;2) Use a sample pen to write the hydrogel precursor on the substrate (such as a glass plate); the writing refers to touching the front end of the pen tip 1 to the substrate, and then driving the pen tip 1 to move on the substrate, so that the ball at the front end of the pen tip 1 The bead 3 rotates ...
Embodiment
[0026] The structure of the biological sampling pen is as follows: figure 1 As shown, it consists of 6 parts. The pen head 1 mainly provides the support point of the ball 3. The pen holder 2 is used to store the hydrogel precursor. The ball 3 provides sufficient shear force when rubbing and rotating on the substrate to drive the hydrogel The precursor 5 flows out, the connecting device 4 is used to connect the pen holder 2 and the pen head 1, and the sealing oil 6 prevents the sample (hydrogel precursor) from volatilizing. When the biological sample pen writes on the substrate (similar to the writing process of a ballpoint pen or gel pen), the ball 3 at the front end of the pen head 1 rotates, driving the hydrogel precursor 5 to flow out.
[0027] Utilize the bio-loading pen to prepare the hydrogel, including the following steps:
[0028] Step 1: Inject an appropriate amount of the desired processed hydrogel precursor (10μL-2mL) into the pen holder, and then seal it with mine...
example 1
[0031] Example 1 Preparation of Photocrosslinked Polyethylene Glycol Microscale Hydrogel Using Biological Loading Pen
[0032] Step 1: Inject 200 μL of polyethylene glycol hydrogel precursor into the pen barrel, which is then sealed with mineral oil to prevent evaporation. The composition of the polyethylene glycol hydrogel precursor is as follows: 10% polyethylene glycol with a molecular weight of 1000, 0.5% 2-hydroxy-2-methyl-1-phenyl-1-propanone, and the balance is water .
[0033] Step 2: Hold the biological sampling pen to write dots on the glass plate.
[0034] Step 3: irradiating with an ultraviolet light source for 30 seconds to cross-link and fix the hydrogel precursor on the substrate to form a point-like micro-scale polyethylene glycol hydrogel ( figure 2 ).
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