Hydrogen peroxide sensor, preparation method thereof, and application thereof in detecting unicellular hydrogen peroxide

A hydrogen peroxide and sensor technology, which is applied to instruments, measuring devices, scientific instruments, etc., can solve the problems that the sensor is not suitable for capillary electrophoresis electrochemical detection, the production of hydrogen peroxide sensor is cumbersome, and the detection limit is not low enough, so as to promote the transfer and production of electrons. Simple, high-sensitivity effects

Inactive Publication Date: 2013-07-10
SHANDONG NORMAL UNIV
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0004] Existing pair H 2 o 2 detection, the sensitivity is not high, the detection limit is not low enough, and the electroactive substances that may exist in cells such as ascorbic acid and cysteine ​​have a negative effect on H 2 o 2 The measurement has interference
[0005] The Chinese patent with the application numbe

Method used

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  • Hydrogen peroxide sensor, preparation method thereof, and application thereof in detecting unicellular hydrogen peroxide
  • Hydrogen peroxide sensor, preparation method thereof, and application thereof in detecting unicellular hydrogen peroxide
  • Hydrogen peroxide sensor, preparation method thereof, and application thereof in detecting unicellular hydrogen peroxide

Examples

Experimental program
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Example Embodiment

[0031] Example 1

[0032] Preparation method of hydrogen peroxide sensor:

[0033] (1) Insert a platinum wire (200μm in diameter and about 5cm in length) into a quartz capillary with a length of about 3cm, an inner diameter of 250μm and an outer diameter of 375μm, with platinum wires exposed at both ends. Coat one end with JC-311 epoxy resin glue, return the platinum wire from the other end to the capillary tube, fill the capillary with the platinum wire coated with epoxy resin glue, and make the platinum wire just exposed to the capillary port. The copper wire polished with metallographic sandpaper and the capillary are glued together with 502 glue, and the contact length is about 1cm. Wrap the exposed platinum wire on the copper wire, and smear the platinum wire and copper wire with silver paste, and dry it at 70°C for 30 minutes. Insert the electrode into a section of quartz glass tube with a length of about 3cm, an inner diameter of about 0.5mm and an outer diameter of about ...

Example Embodiment

[0036] Example 2

[0037] Preparation method of hydrogen peroxide sensor:

[0038] Including steps (1), (2), (3), step (3) the mass ratio of platinum nanoparticles to multi-walled carbon nanotubes is 1:1, 1:3, 1:7, 1:9, and other steps are as follows: Example 1.

Example Embodiment

[0039] Example 3

[0040] Preparation method of hydrogen peroxide sensor:

[0041] Including steps (1), (2), (3), the concentration of platinum nanoparticles / multi-walled carbon nanotubes in the dispersion of platinum nanoparticles / multi-walled carbon nanotubes in step (2) is 3mg / mL and 7mg / mL respectively , 10mg / mL, the other steps are as in Example 1.

[0042] Detection of hydrogen peroxide in single cell

[0043] Self-assembled capillary electrophoresis system: CHI810c electrochemical analyzer (Shanghai Chenhua Instrument Co., Ltd.); 0-30kV high voltage power supply (Shandong Normal University Instrument Factory, Shandong).

[0044] The hydrogen peroxide sensor prepared in Example 1 was used.

[0045] Experimental steps:

[0046] (1) Neutrophil extraction: Take 2mL of fresh anticoagulant blood and 2mL of whole blood and tissue homogenate dilutions and mix them in a 10mL centrifuge tube, and let it stand for 30min at 20-30°C. At this time, a large number of red blood cells settled at ...

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Abstract

The invention relates to a hydrogen peroxide sensor, a preparation method thereof, and application of the hydrogen peroxide sensor in detection of unicellular hydrogen peroxide. The hydrogen peroxide sensor is prepared by loading platinum nano-particles on multi-wall carbon nanotubes by ethylene glycol reduction method and then preparing platinum nano-particle/multi-wall carbon nanotube dispersed liquid therefrom, and finally, dipping a platinum electrode with the dispersed nano-particles/multi-wall carbon nanotubes. The sensor provided by the invention is high in sensitivity, low in detection limit and wide in linear range; the sensor is taken as a detector of capillary electrophoresis; and quantitative determination of H2O2 in single cells is realized.

Description

technical field [0001] The invention mainly relates to a hydrogen peroxide sensor, which utilizes capillary electrophoresis electrochemical method to detect H in a single cell 2 o 2 , belongs to the technical field of bioelectrical analysis chemical detection. Background technique [0002] h 2 o 2 It is one of the more important metabolites in the body. It can pass through the cell membrane and is a relatively stable active oxygen. Many reports have also demonstrated that it is a second messenger of cell signaling. and H 2 o 2 It is closely related to the occurrence, development and apoptosis of tumors. 2 o 2 Assays can diagnose and prevent diseases induced by oxidative stress and damage. [0003] Many analytical methods have been used for H 2 o 2 detection, such as fluorescence, spectrophotometry, electrochemical detection and chemiluminescence. Capillary electrophoresis electrochemical analysis method is widely used in single cell analysis due to its advantages...

Claims

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Application Information

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IPC IPC(8): G01N27/30G01N27/447
Inventor 王晓蕾李玲君马艳芳王军刘冬菊
Owner SHANDONG NORMAL UNIV
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