Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Kit for detecting goat pox virus

A technology of goat pox virus and kit, applied in the direction of microbe-based method, microbe measurement/inspection, microbiology, etc., can solve problems such as inability to distinguish goat pox virus genus, inability to distinguish viruses of the same genus and different species, etc. , to achieve the effect of high sensitivity, high detection sensitivity and simple detection operation

Active Publication Date: 2013-07-24
LANZHOU INST OF VETERINARY SCI CHINESE ACAD OF AGRI SCI
View PDF4 Cites 1 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

It can be seen that the above-mentioned published methods or kits cannot distinguish viruses of the same genus and different species, that is to say, it is impossible to distinguish the genus of goatpox virus from the book of goat pox virus

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Kit for detecting goat pox virus
  • Kit for detecting goat pox virus
  • Kit for detecting goat pox virus

Examples

Experimental program
Comparison scheme
Effect test

Embodiment Construction

[0016] The present invention will be explained in detail below in conjunction with the examples.

[0017] 1. Preparation of Sequences

[0018] The amplification primers of the present invention are designed using relevant primer design software to design the reversed repeat ends (within 1-2000bp) of the sheeppox virus genome, and then select the primers with higher scores from the hundreds of pairs of primers obtained, and then insert the designed The sequence was sent to Nanjing GenScript Bioengineering Co., Ltd. for 5' end modification and synthesis. The sequences that were finalized were:

[0019] SEQ №1: ACCAAAACAAATAATCAGAGATG, named GTPV F3 in the present invention;

[0020] SEQ №2: CCTAATCCATTTAAGACACTACG, named GTPV B3 in the present invention;

[0021] SEQ №3: AAGATGTCTTCCGGTAACTATGTCT-GCGGTTGTGATATCGTCA, named GTPV FIP in the present invention

[0022] SEQ No. 4: CCGAACTTGTTATTTCTTGTGCTT-CACAATAAGGAACCACCTAGA, named GTPV BIP in the present invention.

[0023] 2....

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention discloses a kit for detecting goat pox virus. The kit disclosed by the invention at least comprises four primers of SEQ (sequence) No. 1, SEQ No. 2, SEQ No.3 and SEQ No. 4, and the kit further comprises dNTP (deoxy-ribonucleoside triphosphate), Tris-HC, KCl, (NH4)2SO4, MgSO4, Tween 20 and a Bst (bacillus stearothermophilus) enzyme, as well as template DNA (deoxyribonucleic acid). The primers in the kit disclosed by the invention have specificity and can not amplify the nucleic acid of the goat pox virus under same conditions; and simultaneously, the kit is higher in detection sensitivity and also has the advantages that the detection operation is relatively simple and fast.

Description

technical field [0001] The invention relates to a veterinary detection kit, specifically a kit for detecting goat pox virus. Background technique [0002] Capripox (CP) includes sheep pox, goat pox and bovine skin pimple disease, the first two of which are caused by sheep pox virus (Sheeppox virus, SPPV) and goat pox virus (Goatpox virus, GTPV) respectively. contact infectious diseases. The body temperature of sick animals rises, papules or nodules, blisters, visceral lesions and even death appear on the whole body, which has caused serious economic losses to the sheep industry in the world. The World Organization for Animal Health (OIE) stipulates it as an animal disease that must be reported by law, and my country lists it as a first-class animal infectious disease. The differential diagnosis of sheep pox and goat pox has been a research hotspot. [0003] The differential diagnosis of sheep pox and goat pox has been a research hotspot. Studies have shown that the homo...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
IPC IPC(8): C12Q1/70C12Q1/68C12R1/93
Inventor 张强赵志荀范斌吴国华颜新敏李健朱海霞芦晓立孙晓林刘发央
Owner LANZHOU INST OF VETERINARY SCI CHINESE ACAD OF AGRI SCI
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com