Method for simply and rapidly identifying resistance of pyricularia grisea

Abstract

The invention relates to a method for simply and rapidly identifying the resistance of the pyricularia grisea. The method comprises the steps of: extracting a heading rice plant from the rice field, respectively taking rice internode stems with lengths of not less than 5cm above and below an internode by using the internode of a spike neck for later use; placing degreasing cotton in a moisturizing plastic box, irrigating 8-10ml of spore suspension solution each milliliter of which contains 0.6 millions pyricularia grisea; and placing the rice internode stems to be detected on the degreasing cotton, moisturizing and cultivating for 14 days at a temperature of 25-26 DEG C so that scabs which can be fully identified can be formed, wherein sizes and invasion degrees of the scabs are divided into five levels, the rice internode stems with 0-level or 1-level morphological characteristics are of a disease-resistant type R, the rice internode stems with 4-level morphological characteristics are of a disease-susceptible type S, and the rice internode stems with 2-level or 3-level morphological characteristics are of a middle type M. Identification can be carried out indoors by using a stem node inoculating method, thus errors caused by identification under the natural condition in the field are avoided. The method does not need equipment, and is simple and easy to operate and strong in practicability.

Description

technical field [0001] The invention relates to an agricultural production technology, in particular to a simple and rapid method for identifying rice blast resistance. Background technique [0002] Rice blast is one of the major diseases of rice. Paddy rice losses due to rice blast damage are about 6 billion US dollars every year in the world. In the years when rice blast is prevalent in our country, rice production often decreases by 10-30%, sometimes even more, which seriously affects the high and stable production of rice. Practice has proved that selecting high-yield and disease-resistant rice varieties is an economical and effective measure for controlling rice blast. [0003] In recent years, rice breeding programs in many countries have listed disease resistance, insect resistance, and stress resistance breeding as important goals. However, in order to breed disease-resistant varieties, the first thing is to conduct rapid and large-scale identification of rice va...

AI Technical Summary

Problems solved by technology

Due to the different growth stages of rice varieties, the climatic conditions at the time of inoculation at the booting stage are also different, and the size of the panicle shape of different varieties is different, resulting in different heading speeds and slowness, and the inoculation at the earing stage often leads to misjudgment.