Reticuendotheliosis virus and subgroup-J avian leukosis virus rapid combined-detection test strip

Abstract

The invention discloses a test strip used in one-step rapid detection of reticuendotheliosis virus (REV) and subgroup-J avian leukosis virus (ALV-J). The test strip comprises a non-absorbing supporting layer, and an absorption layer adhered to the supporting layer. The absorption layer is formed by sequentially spliced components of an absorption fiber layer, a gold-labeled antibody fiber layer, a cellulose film layer, and a water absorption layer. The cellulose film layer is marked with anti-goat IgG or anti-mouse IgG control blot, and a detection blot comprising anti-REV and anti-ALV-J antibody. The anti-REV and anti-ALV-J antibody is an anti-REV and anti-ALV-J antibody monoclonal or polyclonal antibody. An anti-REV and anti-ALV-J mixed polyclonal antibody or monoclonal antibody marked by colloidal gold and corresponding to the detection blot is adhered to the gold-labeled antibody fiber layer. The test strip provided by the invention has the advantages of high detection specificity, high sensitivity, simple operation, fast detection, and intuitive result. The test strip is suitable for REV and ALV-J virus on-site rapid combined detection, and can be used in identification and diagnosis of single infection or mixed infection of the two viruses. The test strip can be widely applied, and is suitable for popularization.

Description

[0001] technical field [0002] The invention relates to a device for detecting poultry reticuloendotheliosis and J subgroup avian leukemia, in particular to a joint detection colloidal gold for rapid detection of poultry reticuloendotheliosis virus and J subgroup avian leukemia virus test strips. technical background [0003] Avian reticuloendotheliosis (Reticuloendotheliosis, RE) is caused by avian reticuloendotheliosis virus (Reticuloendotheliosis virus, REV). group of syndromes, including acute reticulosis, short stature syndrome, and chronic neoplastic proliferative disorders of lymphoid and other tissues. REV infection in poultry is ubiquitous, but the symptoms are not obvious, and it can be transmitted among different birds. The infection is characterized by inducing an immunosuppressive state in chicken flocks without obvious pathological changes. The mode of transmission of REV in chickens and other susceptible birds can not only be transmitted horizontally throu...

AI Technical Summary

Problems solved by technology

The detection cost of this kind of method is relatively high, and the test operation is more complicated, and it is difficult for general farm staff to master

Molecular biology methods such as PCR, RT-PCR, and fluorescent quantitative PCR are mainly used for the detection of viral nucleic acids. Although these techniques can accurately differentiate REV and ALV-J, the operation is complicated and requires specific reagents and expensive equipment. Instruments and equipment have high requirements for operation, so it is difficult to be suitable for rapid differential diagnosis on the production line or on the scene of epidemic diseases

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