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Screening method of salt resisting germplasms of corn

A screening method and salt-tolerant technology are applied in the field of screening of salt-tolerant inbred lines of maize, which can solve the problems of prolonging the experimental time, increasing the screening workload, reducing the experimental efficiency, etc., so as to improve the screening efficiency, shorten the screening time, and achieve high application effect of value

Active Publication Date: 2013-08-14
安徽英诺管理咨询有限公司
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Problems solved by technology

Although these methods can be used to screen salt-tolerant maize germplasm, it will increase the workload of screening, and the determination of physiological and biochemical indicators of maize germplasm is more complicated, and the experimental error is large. To obtain accurate and reliable salt-tolerant germplasm, it needs to be repeated many times. experiment, thus prolonging the experiment time and reducing the experiment efficiency

Method used

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  • Screening method of salt resisting germplasms of corn
  • Screening method of salt resisting germplasms of corn
  • Screening method of salt resisting germplasms of corn

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experiment example

[0021] Experimental example, the method for the selection of maize salt-tolerant germplasm, is carried out according to the following steps:

[0022] 1. Collection of materials: Three maize germplasm materials of 81162, 8723 and P138 were collected for the experiment.

[0023] 2. Seed disinfection: select undamaged seeds of the same size, sterilize with 0.5% sodium hypochlorite for 10 min, rinse with distilled water for 3 times, and dry the attached water with filter paper.

[0024] 3. Seed soaking under salt stress: After the seeds were sterilized, soak the seeds in 0 mmol / L, 60 mmol / L, 140 mmol / L, 220 mmol / L NaCl solutions for 12 h, and the seed soaking treatment in distilled water was used as the control;

[0025] 4. Seed germination experiment: The seeds treated in step 3 were placed in a petri dish with a diameter of 12 cm, and double-layered filter paper was used as a germination bed, with 30 seeds per dish. 0 mmol / L, 60 mmol / L, 140 mmol / L and 220 mmol / L NaCl solutions ...

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Abstract

The invention relates to a screening method of salt resisting germplasms of corns. The method comprises the following steps: after seeds are disinfected, soaking the seeds in saline liquor with different concentrations for 12 hours for a germinating and seedling growth experiment; in a stress experiment in a germinating stage, supplementing 15ml of saline liquor for one time every day; after the seeds are germinated for 4 days, retaining the germinating seeds which are consistent in growth for each sample, and determining the content of Na+ and K+ ions; in the seedling growth experiment, directly seeding the corn seeds disinfected and soaked in disinfected vermiculites containing 0mmol / L, 60mmol / L, 140mmol / L and 220mmol / L NaCl liquids; placing the seeds in a greenhouse for stress treatment; and determining the content of Na+ and K+ ions when the controlled seedling has three leaves and one heart. According to the method provided by the invention, the salt resisting germplasms are screened through the Na+ and K+ ions and K+ / Na+ ratio in parts of different selfing lines in the germinating stage and seedling growth stage, so that the screening workload is effectively reduced, the screening time is shortened, and the screening efficiency and the accuracy of the result are improved.

Description

technical field [0001] The invention belongs to the technical field of breeding of new agricultural varieties, and in particular relates to a screening method for salt-tolerant inbred lines of corn. technical background [0002] 67 million hm in my country 2 10% of cultivated land is saline soil, and soil salinization has become one of the important environmental factors that limit the further increase of crop yield. Maize (Zea mays) is moderately sensitive to salinity and relatively poor in salt tolerance. Therefore, in-depth study of the physiological mechanism of salt tolerance in corn, screening of salt-tolerant varieties, and breeding of corn with a certain salt-tolerant ability are of great significance for making full use of salinized land and increasing corn yield. [0003] There have been a lot of reports on the screening of corn salt-tolerant germplasm. Meng Yijiang et al. screened 36 maize hybrids for salt tolerance, and 3 varieties with strong salt tolerance w...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01C1/00A01C1/04
Inventor 彭云玲赵小强闫慧萍
Owner 安徽英诺管理咨询有限公司
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