Soil heavy metal biodegradation agent and preparation method thereof

A biodegradant and heavy metal technology, applied in the direction of microorganism-based methods, biochemical equipment and methods, microorganisms, etc., can solve the problems of reducing soil fertility, insignificant effect, and complicated implementation, so as to improve soil and benefit human health , Improve the effect of water retention, fertilizer retention and air permeability

Active Publication Date: 2013-08-21
金葵子环保生物科技(国际)有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

One is engineering treatment, such as digging and replacing soil, but the implementation is complicated, the cost is high and the soil fertility is easy to be reduced
The second is chemical treatment, the effect and cost are moderate, the disadvantage is that it is easy to reactivate
The third is agricultural management, which is easy to operate and low in cost, but the cycle is long and the effect is not significant

Method used

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  • Soil heavy metal biodegradation agent and preparation method thereof
  • Soil heavy metal biodegradation agent and preparation method thereof
  • Soil heavy metal biodegradation agent and preparation method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0032] The main components of a soil heavy metal biodegradation agent are strains WH4, strain WH15, strain WH18, strain WH20, charcoal, active organic materials, and vermiculite.

[0033] Among the above microorganisms, the strain WH4 is Bacillus Licheniformis; the strain WH15 is Pseudomonas taiwanensis; the strain WH18 is Streptomyces microflavus; the strain WH20 is Pseudomonas aeruginosa Pseudomonas Aeruginosa.

[0034] The ratio of bacteria contained in the soil heavy metal biodegradant is (percentage by weight):

[0035] (1) WH4 strain: 10%; (2) WH15 strain: 13%;

[0036] (3) WH18 strain: 40%; (4) WH20 strain: 37%.

[0037] The manufacture method of this soil heavy metal biodegradant comprises strain cultivation and preparation, and its process step is:

[0038] (1) The above WH4 strains, WH15 strains, WH18 strains, and WH20 strains are uniformly mixed according to the above weight percentage to form a WH strain;

[0039] (2) WH flora secondary medium formula (weight p...

Embodiment 2

[0048] The proportioning and parameter of each composition of the soil heavy metal biodegradant of the present embodiment are different from example 1:

[0049] 1. The proportion of bacteria contained in the soil heavy metal biodegradation agent is (weight percent):

[0050] (1) WH4 strain: 26%; (2) WH15 strain: 50%;

[0051] (3) WH18 strain: 8%; (4) WH20 strain: 16%.

[0052] 2. The ratio (percentage by weight) of the secondary strain culture medium is: active organic material 35%, rice bran 45%, soybean powder 17%, milk powder 1.5%, sugar 0.5%, and the rest water.

[0053] 3. The temperature of the secondary strain culture medium is 45°C, the pH value is 6.5, and the culture time is 10 days, and the number of viable bacteria can reach 1.5 billion / g.

[0054] 4. Mix charcoal, active organic materials, vermiculite and active furfur of WH flora according to the following formula by weight percentage, ferment at room temperature for more than 10 days, pulverize, and pack to ma...

Embodiment 3

[0061] The proportioning of each composition of the soil heavy metal biodegradant of the present embodiment and each parameter and example 1 difference are:

[0062] 1. The proportion of bacteria contained in the soil heavy metal biodegradation agent is (weight percent):

[0063] (1) WH4 strain: 45%; (2) WH15 strain: 30%;

[0064] (3) WH18 strain: 17%; (4) WH20 strain: 8%.

[0065] 2. The ratio (percentage by weight) of the secondary strain culture medium is: active organic material 45%, rice bran 35%, soybean powder 15%, milk powder 2.5%, sugar 1.5%, and the rest water.

[0066] 3. The temperature of the medium for secondary strains is 30°C, the pH value is 7.5, the culture time is 15 days, and the number of viable bacteria can reach 500 million / g.

[0067] 4. Mix charcoal, active organic materials, vermiculite and active furfur of WH flora according to the following formula by weight percentage, ferment at room temperature for more than 10 days, pulverize, and pack to make...

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Abstract

The present invention discloses a soil heavy metal biodegradation agent and a preparation method thereof. Main components of the soil heavy metal biodegradation agent comprise WH4 bacteria, WH15 bacteria, WH18 bacteria, WH20 bacterial, charcoal, an active organic material and vermiculite. The preparation method comprises the following steps: mixing the WH4 bacteria, the WH15 bacteria, the WH18 bacteria and the WH20 bacteria according to a certain ratio to obtain a WH flora, adopting a WH flora second-class culture medium to carry out second-class bacterial culture on the WH flora, mixing the charcoal, the vermiculite, the active organic material and active bran bacterial of the WH flora according to the formula, carrying out room temperature fermentation for more than 10 days, and carrying out grinding and packaging to prepare the soil heavy metal biodegradation agent. With application of the soil heavy metal biodegradation agent in toxic heavy metal degradation in soil, significant effects are provided.

Description

technical field [0001] The invention relates to the technical field of soil heavy metal biodegradation, in particular to a soil heavy metal biodegradation agent and a manufacturing method thereof. Background technique [0002] From the end of the last century to the beginning of this century, we Asians suffered the most serious damage from heavy metals after we entered industrialization. First of all, rice in Japan is polluted by cadmium and has sequelae such as itai disease. Taiwan is no exception. Therefore, at the turn of this century, the research on heavy metal pollution has received special attention. For example, the cadmium content in brown rice of indica rice line among rice varieties is higher than that of japonica rice. Among the heavy metals, nickel and lead are easier to diffuse than other elements, and they intrude from the water inlet and outlet. The international rice safety quality standard for heavy metals in Taiwan should be reduced from the current 0....

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20B09C1/10C09K17/14C12R1/10C12R1/38C12R1/385C12R1/465C09K101/00
Inventor 何飚黄恩雄何定国何定荣
Owner 金葵子环保生物科技(国际)有限公司
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