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A kind of method that utilizes common fermenter to prepare coated microcapsule before microbial fermentation

A technology of microbial fermentation and microencapsulation, which is applied in the direction of fixing on or in the inorganic carrier, fixing on/in the organic carrier, etc., can solve the problem that the degree of mechanization and automation cannot meet the requirements and affect the shape of the product (sphericity yield) , increase the cost and complexity of the device, and achieve the effects of avoiding low microencapsulation efficiency and yield, controllable cell density, and good shape and stability

Active Publication Date: 2014-10-22
ACAD OF NAT FOOD & STRATEGIC RESERVES ADMINISTRATION
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, the production equipment of biological microcapsules coated before fermentation, except that the equipment of extrusion spraying is relatively mature and can meet the needs of industrial production, other methods cannot meet the requirements in terms of work efficiency and degree of mechanization and automation.
The squeeze spray method requires special nozzles, air compression and other devices. In the production process, the nozzles and air compression devices will not only increase the complexity and cost of the equipment, but also due to the low production efficiency of a single nozzle, usually in a preparation tank Installing multiple nozzles in the body not only further increases the cost and complexity of the device, but also often leads to poor passage of film-forming materials during the preparation of microcapsules, affecting product shape (sphericity) and yield, and the product particle size is too large ,hard to control

Method used

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  • A kind of method that utilizes common fermenter to prepare coated microcapsule before microbial fermentation
  • A kind of method that utilizes common fermenter to prepare coated microcapsule before microbial fermentation
  • A kind of method that utilizes common fermenter to prepare coated microcapsule before microbial fermentation

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Embodiment 1

[0025] Embodiment 1: 50 liters of fermentation tanks produce yeast microcapsules

[0026] 1. Preparation of seed solution: inoculate the preserved yeast strains in YPD medium, culture in shake flasks at 30±1°C for 8-10 hours, until the yeast grows to the end of logarithmic growth, stop the cultivation for later use.

[0027] 2. Preparation of capsule material solution: Add 3L of water into the feeding tank, weigh 45g of food-grade sodium alginate, add it to the feeding tank, stir until completely dissolved, and prepare a sodium alginate solution with a concentration of 15g / L , sterilized at 115°C for 15-20 minutes.

[0028] 3. Preparation of the aqueous phase mixture: weigh 45g of CaCO2 in a ratio of 1:1 to the mass ratio of the sodium alginate used. 3 , put into the feeding tank, mix evenly, steam sterilize at 115°C for 20 minutes, cool to about 35°C, add microbial seed liquid at the end of logarithmic growth, and cultivate to a density of 10 6 About cfu / ml, mix well again ...

Embodiment 2

[0034]Embodiment 2: the yeast microcapsule produced by 1000 liters of fermentation tanks

[0035] 1. Preparation of seeds: inoculate the preserved yeast strains in YPD medium, and culture in shake flasks at 30±1°C for 8-10 hours until the yeast grows to the end of logarithmic growth, stop the cultivation for later use.

[0036] 2. Preparation of capsule material solution: Add 60L of water into the feeding tank, weigh 1.2kg of food-grade sodium alginate, add it to the feeding tank, stir until completely dissolved, and prepare sodium alginate with a concentration of 20g / L Solution, sterilized at 115°C for 15-20 minutes.

[0037] 3. Preparation of the aqueous phase mixture: Weigh 1.8kg of CaCO2 at a ratio of 1:1.5 to the mass ratio of the sodium alginate used 3 , put it into the feeding tank, mix well, steam sterilize at 115°C for 25 minutes, cool to about 38°C, add microbial seed liquid at the end of logarithmic growth, and reach 10 6 The density is about cfu / ml, and fully mix...

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Abstract

The invention discloses a method for preparing a coating microcapsule before microbial fermentation by utilizing a common fermentation tank. The method comprises steps of: preparation of a capsule material solution, preparation of an aqueous mixed solution, preparation of an oil mixed solution, preparation of a settling agent, preparation of microcapsules and culturing after coating, etc. By the method, microcapsules of which the diameter is 100-400 micrometers can be prepared, and the microcapsules have good sphericity degree and the yield of the product is higher than 95%. The innovation point of the method disclosed by the invention is that the method utilizes the general fermentation tank for preparation and fermentation of microcapsules without any modification, really realizes large-scale production of probiotic microcapsules, and has better product yield, sphericity degree and dispersibility than those of the existing industrial before-fermentation coating production method. The method disclosed by the invention is suitable for preparation of microorganism microcapsule in a plurality of fields such as fodders, foods and medicines, etc.

Description

technical field [0001] The invention relates to a method for preparing coated microcapsules before microbial fermentation by using a common fermenter, belonging to the field of biotechnology. Background technique [0002] Microencapsulation technology has achieved good results in protecting bioactive molecules, tissues and cells against adverse environments. Although microcapsule technology has been widely used in medicine, chemical industry, food and other fields, there are still bottlenecks in the large-scale production of microcapsules, especially coated cells, including microbial cells and animal cells, resulting in certain restrictions on its application. . Industrial production equipment should have low production cost, high production efficiency, and reduce manual operations as much as possible. At present, the production equipment of biological microcapsules coated before fermentation, except that the equipment of extrusion spraying is relatively mature and can mee...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N11/14C12N11/10C12N11/04
Inventor 李爱科綦文涛梁新晓贠婷婷付亭亭王永伟
Owner ACAD OF NAT FOOD & STRATEGIC RESERVES ADMINISTRATION
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