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Creation method of cotton fiber length single QTL near-isogenic line

A near-isogenic line and cotton fiber technology, applied in the field of molecular breeding, can solve problems affecting the estimation of genetic effects of fine mapping of target QTL

Inactive Publication Date: 2013-09-04
JIANGSU RUIHUA AGRI SCI & TECHCO
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, in addition to the target chromosomal region, near-isogenic lines usually contain a certain number of donor chromosome segments, and these genetic backgrounds will have an impact on the effect of the target QTL, thereby affecting the fine mapping of the target QTL and the estimation of the genetic effect

Method used

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  • Creation method of cotton fiber length single QTL near-isogenic line
  • Creation method of cotton fiber length single QTL near-isogenic line
  • Creation method of cotton fiber length single QTL near-isogenic line

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0018] Example 1: Near-equivalence analysis of the introgression line R01-40-08

[0019] The specific method is as follows: In order to analyze the near equivalence of the introgression line R01-40-08. Based on two published cotton genetic linkage maps (Guo et al., 2007; Xiao et al., 2009), we selected 534 SSR primers distributed throughout the cotton genome to analyze introgression line R01-40-08 and donor Polymorphism between parental Pima S-6 and recurrent parent Tamcot2111. The results showed that there were 413 primers showing polymorphism between the donor parent Pima S-6 and the recurrent parent Tamcot2111, among which 23 primers showed polymorphism between R01-40-08 and the recurrent parent Tamcot2111. Among them, there are 11 on chromosome 1 (BNL2921, JESPR240, NAU422, MUSS84, MUSS422, CIR018, JESPR56, NAU2182, TMD03 and BNL3090 and 1 pair of STS primers (STS38), 1 on chromosome 2 (NAU2858), and 1 on chromosome 3 (NAU1167), 3 chromosome 14 (NAU2190, NAU3820, AU5465)...

Embodiment 2

[0020] Example 2: Construction of high-density genetic linkage map in the vicinity of qFL-chr1

[0021] The specific method is as follows: the Pima S-6 fragment carried on the chromosome of the initial introgression line R01-40-081 is located in the primer

[0022] Between NAU3384-BNL3090. In order to enrich the target fragment interval, we selected 88 SSR primers from chromosome 1 to analyze the introgression line R01-40-08 and the recurrent parent Tamcott2111. As a result, 12 pairs of polymorphic primers (NAU3384, MGHES10, NAU3533, CGR5144, CIR049, NAU5085, DPL0887, NAU4891, BNL2827, DPL094, CGR5914 and TMB0062) were obtained. In addition to the 10 pairs of SSR primers (BNL2921, JESPR240, NAU422, MUSS84, MUSS422, CIR018, JESPR56, NAU2182, TMD03 and BNL3090) and 1 pair of STS primers (STS38) obtained in the previous study (Chee et al., 2005), a total of 23 The pair of primers are located within the R01-40-08 target segment. A F 2 group. Using the above 23 pairs of polymo...

Embodiment 3

[0023] Example 3: Substitution Mapping of Recombined Individuals in the Target Interval

[0024] The specific method is as follows: analyze the F2 population with 23 polymorphic primers obtained in Example 2, and identify the target interval altogether

[0025] NAU3384-BNL3090 marked 432 individuals with interval recombination. Among the 432 recombinant individuals, 143 contained different heterozygous fragments of Pima S-6 under the homozygous Tamcot2111 genetic background; 289 contained different heterozygous fragments under the homozygous Pima S-6 genetic background. In view of the fact that the Pima S-6 genetic background may have an impact on the phenotype, we did not consider the recombined individuals under the Pima S-6 genetic background when fine-mapping, and carried out multi-year, multiple-repeated studies on 143 recombined individuals from the Tamcot2111 genetic background. fiber length measurement.

[0026] According to the different recombination positions, we ...

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Abstract

The invention relates to a greation method of a cotton fiber length single QTL (Quantitative Trait Loci) near-isogenic line. The method comprises the steps of adopting introgression lines, with co-fiber length QTL, containing sea island cotton (G.barbadense) pima S-61 chromosome and recurrent parent TAMCOT2111 to establish an F2 group, so as to establish a genetic linkage map in a target QTL interval, then identifying recombinant individual and measuring the fiber length, performing displacement graphic construction according to identification results, and positioning the target QTL marked interval; and selecting recombinant individual and recurrent parent to perform backcross in the F3 group, and performing foreground and background molecular marker assistant selection and fiber length identification, so as to obtain the single QTL near-isogenic line with genetic background identical with recurrent parent and the fiber length which is obviously superior to that of recurrent parent. The method obtains a co-fiber length signle QTL near-isogenic line of cotton No.1 chromosome, and creates an important material for the accurate positioning of target QTL and subsequent map-based cloning.

Description

technical field [0001] The invention belongs to the technical field of molecular breeding, and in particular relates to a method for creating near-isogenic lines with a single QTL for cotton fiber length. Background technique [0002] Cotton is an important economic crop in the world, as well as the most important natural fiber crop and raw material for textile industry. With the introduction of high-efficiency and fast spinning technologies such as air-jet spinning and air-end spinning in the textile sector, and the general improvement of consumers' clothing requirements, higher requirements have been placed on the fiber quality of my country's cotton varieties. Fiber length is one of the important indicators for evaluating fiber quality, and it ultimately affects the quality of end products. The improvement of fiber length by conventional breeding is slow. [0003] More importantly, important quality indicators such as fiber length and fiber strength have a significant ne...

Claims

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Application Information

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IPC IPC(8): C12Q1/68
Inventor 徐鹏高进沈新莲张香桂范昕琦
Owner JIANGSU RUIHUA AGRI SCI & TECHCO
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