Application of FITC-dextran in manufacturing fundus angiographic agent

A technology of angiography and dextran, which is applied in the field of application of FITC-dextran in the preparation of fundus angiography, can solve the problems of application limitations of ICG fundus angiography, and achieve high resolution and high definition Effect

Inactive Publication Date: 2013-09-11
倪明 +2
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Due to the above defects and negative effects, the application of ICG fundus angiography has been limited

Method used

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  • Application of FITC-dextran in manufacturing fundus angiographic agent
  • Application of FITC-dextran in manufacturing fundus angiographic agent
  • Application of FITC-dextran in manufacturing fundus angiographic agent

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0064] FITC-dextran fundus angiography: Simultaneous observation of retinal and choroidal vessels in the same fundus angiogram image.

[0065] Materials and methods: The test subjects were healthy rabbits weighing 1.8-2.5 kg. FITC-dextran (FD-70S, Sigma, St. Louis, MO) with an average molecular weight of 70 kDa was used as a contrast agent. Dissolve FITC-dextran in water to obtain a solution with a concentration of 25 mg / ml. Each rabbit was intravenously injected with 0.3 ml of FITC-dextran solution. Angiographic images were acquired using a conventional fundus fluorescence system (Topcon 50 VT).

[0066] figure 1 The results in show FICT-dextran fundus angiography images of a normal rabbit eye. figure 1 Red arrows in A, B, and C indicate normal retinal vessels, in figure 1 Yellow arrows in A, B, C, and D indicate normal choroidal vessels, suggesting that FITC-dextran angiography allows simultaneous visualization of retinal and choroidal vessels in the same fundus angi...

Embodiment 2

[0068] Effective Imaging Duration of Rabbit FITC-dextran Fundus Angiography

[0069]Materials and methods: FITC-dextran with an average molecular weight of 70 kDa (FD-70S, Sigma Corporation, St. Louis, Missouri) was dissolved in water for injection to a final concentration of 25 mg / ml. Dutch Belted rabbits were injected with a volume of 0.3 ml of the above solution through the marginal vein. Fluorescence images of choroidal vessels were monitored for at least 2 hours after injection of FITC-dextran solution, and choroidal angiography images were acquired using a conventional fundus fluorescence imaging system (Topcon 50 VT).

[0070] figure 2 Fluorescence images of choroidal vessels within 2 hours after a single intravenous injection of FITC-dextran solution are shown. figure 2 Panels A to H show contrast images of normal choroidal vessels acquired at 10 s, 30 s, 1 min, 1.4 min, 10.4 min, 32.6 min, 59.3 min, and 119.4 min after intravenous injection of FITC-dextran.

[00...

Embodiment 3

[0073] Application of FITC-dextran fundus angiography in rabbit eyes with retinal detachment

[0074] Materials and methods: Retinal detachment was induced by subretinal injection of Healon in the eyes of pigmented rabbits. FITC-dextran (FD-70S, Sigma, St. Louis, MO) with an average molecular weight of 70 kDa was dissolved in water for injection to a final concentration of 25 mg / ml. Rabbits were injected intravenously with a volume of 0.3 ml of the above solution through the marginal vein. Choroidal angiography images were acquired using a conventional fundus fluorescence system (Topcon 50VT).

[0075] Fundus angiography images of retinal detachment in rabbit eyes were obtained by FITC-dextran fundus angiography. image 3 The yellow arrow in indicates the area of ​​retinal detachment with sharp edges, and the image also shows fluid in the subretinal space at the site of retinal detachment and choroidal vessels underlying the area of ​​retinal detachment.

[0076] FITC-dextr...

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Abstract

A novel fundus angiographic application and method using fluorescein isothiocyanate dextrans (FITC-dextran) as fluorescence angiographic dye for diagnosis and evaluation of eye diseases on humans is disclosed. The method involves the process of constitution of FITC-dextran solution and intravenous injection of the said solution, followed by observation and photography of ocular fundus circulation using a fluorescence fundus camera with an image system. The invention offers improved fundus angiograms over the traditional Na-fluorescein fundus angiography (Na-fluorescein) and indocyanine green fundus angiography (ICG) with the advantages of higher clarity and higher resolution; capable of visualizing both the retinal and the choroidal circulations systems simultaneously; providing longer fundus image duration so that it can be used as a directly mapping guidance in retinal surgery or to complete the angiograms for both eyes by a single process; providing more information about the pathological changes occurred among retinal, sub-retinal and choroidal circulation systems.

Description

[0001] Cross References to Related Applications [0002] This application claims priority to US Provisional Application No. 61 / 577,009, filed December 17, 2011, the disclosure of which is incorporated herein by reference. technical field [0003] The present invention relates to fluorescein angiography in ophthalmology, in particular to a new diagnostic application and method using fluorescein isothiocyanate dextran (FITC-dextran) as a contrast agent for fundus angiography. To observe and evaluate the circulatory system of normal eyes or diseased eyes of humans or other animals. Background technique [0004] Fluorescein fundus angiography, also known as fundus angiography, is a traditional clinical diagnostic technique for observing fundus blood vessels to diagnose eye diseases. The general process of fundus angiography includes intravenous injection of contrast agent, and then using a fundus camera to take pictures of the fluorescence image of the fundus to obtain a contra...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K49/00
CPCA61K49/0043A61K49/0054A61B3/1241
Inventor 倪明徐坤林军
Owner 倪明
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