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Method of separating anthocyanin from purple highland barley

A technology of anthocyanin and purple, which is applied in the field of extraction of natural pigment anthocyanin, can solve problems such as waste, and achieve the effects of improving utilization rate, reducing damage, and delaying aging

Active Publication Date: 2013-09-11
西藏月王藏药科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, the development of purple highland barley is mainly concentrated on the starch component, while the highland barley bark containing physiologically active components such as anthocyanins are mostly wasted as by-products

Method used

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  • Method of separating anthocyanin from purple highland barley
  • Method of separating anthocyanin from purple highland barley

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Experimental program
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Effect test

Embodiment 1

[0031] The present invention separates the method for anthocyanin from purple highland barley, comprises the following steps:

[0032] 1) Select clean purple highland barley without deterioration and mildew, and use peeling machine peeling technology to collect the outer skin part of purple highland barley;

[0033] 2) Crush the purple highland barley bark, and carry out micro-grinding treatment, so that the particle size is between 0.1-0.2mm;

[0034] 3) Add water equal to the weight of the purple highland barley skin to the purple highland barley skin, then add citric acid to adjust the pH to 3.5, and soak at 20°C for 2 hours;

[0035] 4) Add 70% ethanol solution to the soaked purple highland barley bark at a mass ratio of 1:1 to extract 3 times, each time for 2 hours, filter, combine the filtrate, and then place it at room temperature for 2 hours at 30°C to obtain the extract;

[0036] 5) Ultrasonic extraction: add 1% glucose, 1% acetic acid, and 2% natural flavonoids such...

Embodiment 2

[0042] The present invention separates the method for anthocyanin from purple highland barley, comprises the following steps:

[0043] 1) Select clean purple highland barley without deterioration and mildew, and use peeling machine peeling technology to collect the outer skin part of purple highland barley;

[0044] 2) Crush the purple highland barley bark, and carry out micro-grinding treatment, so that the particle size is between 0.2mm;

[0045] 3) Add water 1.5 times the weight of the purple barley barley barley barley barley, then add citric acid to adjust the pH3, soak at 30°C for 1 hour;

[0046] 4) Add 90% ethanol solution to the soaked purple highland barley bark at a mass ratio of 1:1 to extract twice, each time for 3 hours, filter, combine the filtrate, and then place it at room temperature for 3 hours at 20°C to obtain the extract;

[0047] 5) Ultrasonic extraction: Add 2% glucose, 0.5% acetic acid, and 3% natural flavonoids such as phlorizin to the extract, then ...

Embodiment 3

[0053] The present invention separates the method for anthocyanin from purple highland barley, comprises the following steps:

[0054] 1) Select clean purple highland barley without deterioration and mildew, and use peeling machine peeling technology to collect the outer skin part of purple highland barley;

[0055] 2) Crush the purple highland barley bark, and carry out micro-grinding treatment, so that the particle size is between 0.2mm;

[0056] 3) Add water 1.5 times the weight of the purple barley barley to the purple barley barley, then add citric acid to adjust the pH3, soak at 25°C for 2 hours;

[0057] 4) Add 80% ethanol solution to the soaked purple highland barley bark at a mass ratio of 1:1 to extract twice, each time for 3 hours, filter, combine the filtrate, and then place it at room temperature for 2 hours at 25°C to obtain the extract;

[0058] 5) Ultrasonic extraction: Add 1.5% glucose, 0.8% acetic acid, and 2.5% natural flavonoids such as naringin to the ext...

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Abstract

The invention belongs to an extraction method of natural pigment anthocyanin and particularly relates to a method of separating anthocyanin from purple highland barley. The method comprises the following steps of: crushing purple highland barley skins and performing micro-crushing treatment on the purple highland barley skins; adjusting the pH to 3-3.5 and soaking; ethanol leaching; ultrasonic extracting; concentrating; resin adsorbing; and refining the anthocyanin pigment. The method disclosed by the invention has the beneficial effects of adding additive glucose in the leach liquor before the concentration step to glycosylate the leach liquor, adding acetic acid to acylate the leach liquor so as to stabilize the anthocyanin structure, and adding natural flavone substance to perform a co-pigment function to protect the anthocyanin structure so as to reduce the damage of the anthocyanin and improve the extraction yield of the anthocyanin.

Description

technical field [0001] The invention belongs to a method for extracting natural pigment anthocyanin, in particular to a method for extracting anthocyanin from purple highland barley. Background technique [0002] Anthocyanins are a kind of natural water-soluble pigments, which widely exist in the cell fluid of roots, stems, leaves, flowers, fruits and other organs of plants, so that they appear red, blue or purple. Anthocyanins have certain nutritional and pharmacological effects, and have great application potential in the fields of food, cosmetics, and medicine, and are ideal materials to replace synthetic pigments. Anthocyanins are flavonoids - a group of compounds that can appear red in a class of substances based on the flavonoid core. Due to its unique functionality, as a natural pigment, it is safe, non-toxic, and has many health functions for the human body , used to scavenge free radicals in the body, anti-tumor, anti-cancer, anti-inflammatory, prevent diabetes, lo...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07H17/065C07H1/08C09B61/00
CPCC09B61/00
Inventor 赵辉万强刘敏
Owner 西藏月王藏药科技有限公司
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