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Rapid characterization method for residual-sludge alkaline anaerobic fermentation process and for products of the fermentation process

A technology for anaerobic fermentation and excess sludge, which is applied in material excitation analysis, fluorescence/phosphorescence, measurement devices, etc., can solve the problem of unstable tyrosine-like fluorescence intensity, etc., and achieves short detection time, simple and fast operation steps. The effect of representation

Inactive Publication Date: 2013-09-11
PEKING UNIV
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Problems solved by technology

During the alkaline anaerobic fermentation process of residual sludge, the fluorescence intensity of the protein-like peak in the fermentation broth can be quickly obtained through three-dimensional fluorescence measurement, which can be divided into tyrosine-like (Ex / Em) according to different excitation Ex / emission Em wavelengths. =275 / 310nm and 275 / 340nm) and tryptophan-like (280 / 340nm) fluorescence, wherein, compared with tryptophan-like fluorescence, the fluorescence intensity of tyrosine-like is less stable under alkaline conditions, therefore, it can be Using the fluorescence intensity of tryptophan-like proteins in the three-dimensional fluorescence spectrum to characterize the content of dissolved organic matter in the residual sludge alkaline anaerobic fermentation broth, and there is no detailed report on this aspect so far

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  • Rapid characterization method for residual-sludge alkaline anaerobic fermentation process and for products of the fermentation process
  • Rapid characterization method for residual-sludge alkaline anaerobic fermentation process and for products of the fermentation process
  • Rapid characterization method for residual-sludge alkaline anaerobic fermentation process and for products of the fermentation process

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Embodiment Construction

[0051] Specific embodiments of the present invention will be described below in conjunction with the accompanying drawings.

[0052] Source of excess sludge: The excess sludge is taken from the sludge storage tank of a domestic sewage treatment plant in Langfang City, Hebei Province. The remaining sludge was placed in a 4°C freezer, and after standing still for 24 hours, the initial conditions of the sludge were determined: the sludge concentration (mixed liquor suspended solids) was 12.2g / L; the proportion of mixed liquor volatile suspended solids was 55 %; the total extracellular protein and carbohydrate content were 2.9g / L and 0.6g / L; the initial pH of the sludge was 6.8-7.1.

[0053] Conditions for hydrolysis and acidification of excess sludge: pH9.0±0.2, room temperature 25°C, mechanical stirring speed 60rpm, dissolved oxygen concentration<1mg / L.

[0054] Flow cytometric analysis followed the steps described in the method of the first aspect of the invention (see Summary...

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Abstract

The invention discloses a rapid characterization method for a residual-sludge alkaline anaerobic fermentation process and for products of the fermentation process. The method is characterized by combining flow cytometry and three-dimensional fluorescence spectrum. By utilization of the flow cytometry, cell state changes in sludge during the residual-sludge alkaline anaerobic fermentation process are rapidly characterized, and main sources of soluble substances are determined. By utilization of the three-dimensional fluorescence spectrum, content changes of main soluble organic substances (carbohydrates, proteins, etc.) in alkaline fermentation broth are rapidly characterized. By combining the two technologies, a simple, rapid, and effective characterization method for residual sludge alkaline anaerobic processes is provided. According to the invention, for residual sludge alkaline anaerobic fermentation processes which are aimed at recycling soluble organic substances as denitrifying carbon sources, test processes can be accurately, dynamically and efficiently controlled.

Description

technical field [0001] The invention relates to a method for quickly characterizing the alkaline anaerobic fermentation process and products of surplus sludge, and belongs to the research field of sludge resource utilization and reduction in sewage treatment plants. technical background [0002] "Pollutant Discharge Standards for Urban Sewage Treatment Plants" (GB18918-2002) puts forward stricter requirements for sewage discharge, which requires most sewage treatment plants to carry out advanced treatment of sewage on the basis of existing sewage treatment. During the sewage treatment process, there will be residual sludge (calculated with a water content of 97%) accounting for 0.3-0.5% of the sewage treatment volume. If the advanced treatment is continued, the sludge volume will increase by 0.5-1 times. Therefore, the vigorous development of sewage treatment in our country will make the problem of excess sludge as a by-product of sewage treatment increasingly prominent. ...

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Application Information

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IPC IPC(8): G01N21/64G01N15/14
Inventor 倪晋仁庞丽娜
Owner PEKING UNIV
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