A kind of detection method of human urine hydrocortisone
A technique for urine hydrocortisone and hydrocortisone, which is applied in the field of hydrocortisone detection, can solve the problems of poor anti-interference ability of detection results, insufficient detection sensitivity, many impurity interference substances, etc., and saves dissolution and processing. Time, simple and convenient operation, high sensitivity effect
Active Publication Date: 2016-03-23
南京金域医学检验所有限公司
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[0003] Currently widely used testing techniques for the detection of hydrocortisone concentration in human urine are enzyme-linked immunoassay and high-performance liquid chromatography. These methods have the disadvantages of complicated pretreatment, poor anti-interference ability of test results, and long detection time.
[0004] In addition, there is a technical solution for the detection of urinary hydrocortisone by tandem mass spectrometry, but the positive ion mode is generally used, the detection sensitivity is not high enough, there are many impurities and interferences, and it is difficult to achieve separation under normal conditions
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[0053] In order to make the above objects, features and advantages of the embodiments of the present invention more obvious and comprehensible, the embodiments of the present invention will be further described in detail below in conjunction with specific implementation methods.
[0054] see figure 1 , which shows a flow chart of the steps of an embodiment of a method for detecting human urine hydrocortisone according to an embodiment of the present invention, which may specifically include the following steps:
[0055] Step 101, pre-processing;
[0056] Add methanol solution containing urinary hydrocortisone internal standard to human urine, mix thoroughly and then centrifuge, extract supernatant to obtain the sample to be tested;
[0057] Wherein, the volume ratio of the human urine to the methanol solution can be 1:1; the volume ratio of the supernatant to the corresponding original solution can be 1:2;
[0058] It can be understood that step 101 is a pre-treatment of the...
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The embodiment of the invention provides a method for detecting human urine hydrocortisone. The method comprises the following steps of: adding a methanol solution containing a urine hydrocortisone internal standard substance in the human urine, fully uniformly mixing and then centrifuging and extracting upper-layer supernate to obtain a sample to be tested; detecting the sample to be tested by using a liquid chromatogram series connection quadrupole mass spectrometer; judging the existence of the hydrocortisone according to the relative retention time of the hydrocortisone and the abundance ratio of a hydrocortisone quantification ion pair; calculating the content of the hydrocortisone in the sample according to a peak area ratio of the hydrocortisone to the corresponding internal standard substance on an internal standard curve. The method disclosed by the embodiment of the invention is rapid and efficient, high in sensitivity, strong in specificity, easy to operate and convenient to maintain.
Description
technical field [0001] The invention relates to the technical field of hydrocortisone detection, in particular to a detection method of human urine hydrocortisone. Background technique [0002] Hydrocortisone (cortisol) is a hormone secreted by the adrenal glands. It plays an important role in coping with stress, so it is also known as the "stress hormone". [0003] Currently widely used testing techniques for the detection of hydrocortisone concentration in human urine are enzyme-linked immunoassay and high-performance liquid chromatography. These methods have the disadvantages of complicated pretreatment, poor anti-interference ability of test results, and long detection time. [0004] In addition, there is also a technical solution to detect urinary hydrocortisone by tandem mass spectrometry, but the positive ion mode is generally used, the detection sensitivity is not high enough, there are many impurities and interferences, and it is difficult to achieve separation unde...
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IPC IPC(8): G01N30/88
Inventor 程雅婷赵蓓蓓李卓阳叶文杰何翠红倪周钱俊余木俊陈秀如李梅英黄惠微
Owner 南京金域医学检验所有限公司