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Method for preparing compound enzyme and probiotic preparation for feed by biotransformation of reed and peanut cake

A technology of probiotics preparation and peanut cake, which is applied in the direction of animal feed, animal feed, application, etc., can solve the problem of difficult digestion of monogastric animals, and achieve the effects of low-cost and high-efficiency biotransformation, high enzyme activity, and simple equipment structure.

Inactive Publication Date: 2013-09-18
ZHEJIANG UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Moreover, while the cake is rich in protein, it also contains a relatively high amount of plant fiber, which is not easy for monogastric animals to digest.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0027] 1) Fully mix 10kg of fermented Pu'er cooked tea, 5kg of shiitake mushroom sticks, 5kg of tea tree mushroom sticks with 10kg of reed, 5kg of peanut cake, 2kg of wheat bran, 1kg of apple pomace, and 22kg of water, and acclimate and cultivate at 35°C After 48 hours, a starter was formed;

[0028] 2) Fully mix 20kg of the starter in step 1) with 600kg of reed, 300kg of peanut cake, 60kg of wheat bran, 30kg of apple pomace, and 800kg of water, using solid state fermentation, the thickness of the material is 15cm, natural ventilation, and fermentation at 40 ° C for 4 day, a compound enzyme and probiotic preparation for feed was obtained.

[0029] It is determined that the performance indicators of the compound enzyme and probiotic preparation for feed in this example are: carboxymethyl cellulase activity reaches 48.58IU / g; total cellulase activity reaches 4.87IU / g; beta-glucosidase activity reached 12.12IU / g; endoglucanase activity reached 39.85IU / g; xylanase activity reache...

Embodiment 2

[0031] 1) Fully mix 10kg of fermented Pu'er cooked tea, 5kg of shiitake mushroom sticks, 5kg of tea tree mushroom sticks with 10kg of reed, 5kg of peanut cake, 2kg of wheat bran, 1kg of apple pomace, and 25kg of water, and acclimate and cultivate at 35°C After 48 hours, a starter was formed;

[0032] 2) Fully mix 20kg of the starter in step 1) with 600kg of reed, 300kg of peanut cake, 60kg of wheat bran, 30kg of apple pomace, and 800kg of water, using solid state fermentation, the thickness of the material is 15cm, natural ventilation, and fermentation at 40 ° C for 4 day, a compound enzyme and probiotic preparation for feed was obtained.

[0033] After measurement, the performance indicators of the compound enzyme and probiotic preparation for feed in this example are: carboxymethyl cellulase activity reaches 48.19IU / g; total cellulase activity reaches 4.96IU / g; beta-glucosidase activity reached 12.08IU / g; endoglucanase activity reached 39.64IU / g; xylanase activity reached 4...

Embodiment 3

[0035] 1) Fully mix 10kg of fermented Pu'er cooked tea, 5kg of shiitake mushroom sticks, 5kg of tea tree mushroom sticks with 10kg of reed, 5kg of peanut cake, 2kg of wheat bran, 1kg of apple pomace, and 25kg of water, and acclimatize and cultivate at 40°C After 36 hours, a starter was formed;

[0036] 2) Fully mix 10kg of the starter in step 1) with 600kg of reed, 300kg of peanut cake, 60kg of wheat bran, 30kg of apple pomace, and 1000kg of water, and use solid-state fermentation with a material thickness of 10cm, natural ventilation, and fermentation at 35°C for 5 days , to obtain a compound enzyme and probiotic preparation for feed.

[0037] It is determined that the performance indicators of the compound enzyme and probiotic preparation for feed in this example are: carboxymethyl cellulase activity reaches 47.70IU / g; total cellulase activity reaches 5.22IU / g; beta-glucosidase activity reached 11.84IU / g; endoglucanase activity reached 41.12IU / g; xylanase activity reached 4...

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Abstract

The invention discloses a method for preparing a compound enzyme and probiotic preparation for feed by biotransformation of reed and peanut cake. The method comprises the following steps of: fully mixing Pu'er ripe tea, shiitake mushroom log, agrocybe aegerita mushroom log, first reed, first peanut cake, first wheat bran, first apple pomace and first water which are fermented by piling, and performing acclimation culture so as to form a fermentation agent; and fully mixing the fermentation agent, second reed, second peanut cake, second wheat bran, second apple pomace and second water, and fermenting so as to obtain the compound enzyme and probiotic preparation for feed. In the method disclosed by the invention, a food-grade safe bacterial group with stable microecology is utilized for fermenting the reed and the peanut cake so as to prepare the compound enzyme and probiotic preparation for feed, which has various better performance indexes, and the low-cost and high-efficient biotransformation of the reed and the peanut cake can be realized. Simultaneously, the method disclosed by the invention has the advantages of easiness in growth of microbes, high enzyme activity, extensive fermentation process and easiness in industrial large-scale production.

Description

technical field [0001] The invention belongs to the field of microbial fermentation, and in particular relates to a method for preparing a compound enzyme and probiotic preparation for feed by fermenting reeds and peanut cakes by using microecologically stable food-grade safe flora. Background technique [0002] my country is a populous country with extremely tight arable land, and there is a huge gap in the demand for feed materials, especially energy feed and protein feed. [0003] Lignocellulose is the most abundant and cheapest renewable resource on earth. The production and processing of agricultural products and food also produces a large number of lignocellulosic by-products. There are many kinds of lignocellulose, such as various crop straws, urban fibrous waste, by-products of agricultural product processing such as corn cob, vegetable stems and leaves, fruit and vegetable residues, etc. The components of lignocellulose mainly include cellulose, hemicellulose, lign...

Claims

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Application Information

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IPC IPC(8): A23K1/00A23K1/14
Inventor 阮晖徐娟李青青于骅陈功诸文颖杨璐杜姗姗吴渊纪晓燚
Owner ZHEJIANG UNIV
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