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Preparation method of compound enzymes and probiotics for forage by biotransformation of broccoli rhizome and soybean cake

A technology of probiotic preparation and soybean cake, which is applied in animal feed, animal feed, application, etc., can solve the problems of difficult digestion of monogastric animals, and achieve the effects of easy industrialized large-scale production, low energy consumption, and good economic benefits

Inactive Publication Date: 2013-09-25
ZHEJIANG UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Moreover, while the cake is rich in protein, it also contains a relatively high amount of plant fiber, which is not easy for monogastric animals to digest.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0027] 1) Fully mix 10kg of fermented Pu-erh tea, 5kg of shiitake mushroom sticks, 5kg of tea tree mushroom sticks, 10kg of broccoli rhizomes, 5kg of soybean meal, 2kg of wheat bran, 1kg of apple pomace, and 22kg of water, at 35°C After 48 hours of acclimatization and cultivation, a leavening agent is formed;

[0028] 2) Fully mix 20kg of starter in step 1) with 600kg of broccoli rhizome, 300kg of soybean meal, 60kg of wheat bran, 30kg of apple pomace, and 800kg of water, and use solid-state fermentation with a material thickness of 15cm, with natural ventilation, at 40°C Ferment for 4 days to obtain the compound enzyme and probiotic preparation for feed.

[0029] After determination, the performance indicators of the compound enzyme and probiotic preparation for feed in this embodiment are: carboxymethyl cellulase activity reaches 65.26IU / g; total cellulase activity reaches 6.35IU / g; beta-glucosidase activity reaches 65.26IU / g; reached 13.33IU / g; endoglucanase activity reach...

Embodiment 2

[0031] 1) Fully mix 10kg of fermented Pu-erh cooked tea, 5kg of shiitake mushroom sticks, 5kg of tea tree mushroom sticks, 10kg of broccoli rhizomes, 5kg of soybean meal, 2kg of wheat bran, 1kg of apple pomace, and 25kg of water. After 48 hours of acclimatization and cultivation, a leavening agent is formed;

[0032] 2) Fully mix 20kg of starter in step 1) with 600kg of broccoli rhizome, 300kg of soybean meal, 60kg of wheat bran, 30kg of apple pomace, and 800kg of water, and use solid-state fermentation with a material thickness of 15cm, with natural ventilation, at 40°C Ferment for 4 days to obtain the compound enzyme and probiotic preparation for feed.

[0033] After determination, the performance indicators of the compound enzyme and probiotic preparation for feed in this embodiment are: carboxymethyl cellulase activity reaches 64.18IU / g; total cellulase activity reaches 6.19IU / g; beta-glucosidase activity reaches 64.18IU / g; reached 13.14IU / g; endoglucanase activity reache...

Embodiment 3

[0035] 1) Fully mix 10kg of fermented Pu-erh tea, 5kg of shiitake mushroom sticks, 5kg of tea tree mushroom sticks, 10kg of broccoli rhizomes, 5kg of soybean meal, 2kg of wheat bran, 1kg of apple pomace, and 25kg of water, at 40°C After 36 hours of acclimatization and cultivation, a leavening agent is formed;

[0036] 2) Fully mix 10kg of starter in step 1) with 600kg of broccoli rhizome, 300kg of soybean meal, 60kg of wheat bran, 30kg of apple pomace, and 1000kg of water, and use solid-state fermentation with a material thickness of 10cm, naturally ventilated, and fermented at 35°C After 5 days, a compound enzyme and probiotic preparation for feed was obtained.

[0037] After determination, the performance indicators of the compound enzyme and probiotic preparation for feed in this embodiment are: carboxymethyl cellulase activity reaches 59.60IU / g; total cellulase activity reaches 6.22IU / g; beta-glucosidase activity reaches 59.60IU / g; reached 12.94IU / g; endoglucanase activit...

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Abstract

The invention discloses a preparation method of compound enzymes and probiotics for forage by biotransformation of broccoli rhizome and soybean cake, including the steps of fully mixing Pu'er fermented tea, mushroom stick, agrocybe cylindracea stick, first broccoli rhizome, first soybean cake, first wheat bran, first apple slag and first water after pile fermentation and obtaining a starter after habituated culture; and fully mixing the starter, second broccoli rhizome, second soybean cake, second wheat bran, second apple slag and second water and obtaining a compound enzyme and probiotics for forage after fermentation. In the invention, safe floras at food grade with stable microbiology are used for the fermentation of broccoli rhizome and soybean cake in order to prepare compound enzymes and probiotics for forage that has every good performance index, and the biotransformation of broccoli rhizome and soybean cake has low cost and high efficiency. At the same time, in the invention, microbes are easy to grow; the enzyme activity is high; the fermentation is extensive; and no strict aseptic condition is needed, therefore the invention method is suitable for industrial mass production.

Description

technical field [0001] The invention belongs to the field of microbial fermentation, and in particular relates to a method for fermenting broccoli rhizomes and soybean cakes with micro-ecologically stable food-grade safe flora to prepare a compound enzyme and probiotic preparation for feed. Background technique [0002] my country is a populous country with extremely tight arable land, and there is a huge gap in the demand for feed raw materials, especially energy feed and protein feed. [0003] Lignocellulose is the most abundant and cheapest renewable resource on earth. The production and processing of agricultural products and food also produces a large amount of lignocellulosic by-products. There are many kinds of lignocellulose, such as various crop straws, urban cellulosic waste, agricultural product processing by-products such as corncobs, vegetable stems and leaves, fruit and vegetable residues, etc. The components of lignocellulose mainly include cellulose, hemicel...

Claims

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Application Information

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IPC IPC(8): A23K1/00A23K1/14
Inventor 阮晖徐娟李青青于骅陈功诸文颖杨璐杜姗姗吴渊纪晓燚
Owner ZHEJIANG UNIV
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