Artificial biological tendon and preparation method thereof

A biological and artificial technology, applied in the field of artificial biological tendon and its preparation, can solve the problems that affect the product's anti-infection ability, tissue regeneration ability, product instability, and damage to the three-dimensional structure, so as to increase the DNA removal process, ensure stability and Uniformity, low immunogenicity and high effect

Active Publication Date: 2013-09-18
BEIJING BIOSIS HEALING BIOLOGICAL TECH
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

On the other hand, Biodesign Surgisis products use common hybrid pigs as animal sources, and individual differences in the genetic background of hybrid animals lead to instability and inconsistency in different batches of products
On the other hand, the vacuum compression and

Method used

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  • Artificial biological tendon and preparation method thereof
  • Artificial biological tendon and preparation method thereof
  • Artificial biological tendon and preparation method thereof

Examples

Experimental program
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Effect test

Example Embodiment

[0042] Example 1: Preparation of 8-layer artificial biological tendon

[0043] The preparation method is as follows:

[0044] (1) Determination of animal source and pretreatment and initial washing of small intestine tissue

[0045] The Chinese inbred Wuzhishan mini-pig was selected as the animal source. The animal species was determined using the method specified in the patent ZL200510008994.2. The small intestine tissue of the freshly slaughtered Chinese inbred Wuzhishan mini-pig was cleaned and the submucosa of the small intestine was separated and used Rinse with water for injection 3 times.

[0046] (2) Virus inactivation

[0047] The virus is inactivated by the peracetic acid-ethanol solution method. This step is carried out in a constant temperature ultrasonic cleaner that can be shaken in the cleaning tank. The volume percentage of peracetic acid is 0.05 to 0.2% (preferably 0.1%). The time is 1~2h (preferably 1h), the temperature range is 4~40℃, and then wash 2~5 times in phos...

Example Embodiment

[0056] Example 2: Detection of physical and chemical properties, histology, growth factors and biological properties of the material prepared in Example 1

[0057] 1. The physical properties of the prepared 8-layer materials are tested. The test items include porosity measurement, stitch retention, tensile strength, and blasting strength.

[0058] 1) Porosity measurement: The porosity of the material is measured by mercury intrusion method, and compared with and compared with Biodesign Surgisis products. Results: The porosity of the sample provided in Example 1 was 87.8±2.51, and the porosity of the Biodesign Surgisis product was 78.3±6.38.

[0059] 2) Suture retention test: Method: Use 2-0 surgical suture or stainless steel wire of the same diameter to suture 2 mm within one end of the artificial biological tendon, and fix the suture or stainless steel wire to the other end of the artificial biological tendon. On the tension meter, stretch at a speed of 20mm / min until the suture po...

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Abstract

The invention provides an artificial biological tendon and a preparation method of the artificial biological tendon. Small intestinal sub mucosa tissues of inbred strain animals are adopted as raw materials of the artificial biological tendon and are removed with cell components and deoxyribonucleic acid (DNA) components, extracellular matrix components and structures are completely reserved, and the artificial biological tendon has a cellular structure. The preparation method of the artificial biological tendon comprises the following operation steps of: determining animal sources, preprocessing small intestine tissues, rough cleaning, viral inactivating, removing cells, clearing DNA, forming, packaging and sterilizing. The inbred strain animals are served as an animal source of the artificial biological tendon prepared by the method and have pure, stable and unite genetic characteristics, the stability and uniformity of different batches of products are basically ensured, the DNA residue of the animal source is small, the three-dimensional structure of the natural extracellular matrix component (ECM) is completely reserved, and the artificial biological tendon has low immunogenicity and strong anti-infection capability.

Description

technical field [0001] The invention relates to the technical field of animal-derived implantable medical devices, in particular to an artificial biological tendon and a preparation method thereof. Background technique [0002] Tendon is a kind of viscoelastic tissue, which can transmit the force generated by muscle to bone and cause the movement of limb. The biological properties of tendons affect the contractility and sports performance of muscles to a certain extent. Tendon damage from exercise or disease often leads to limb dysfunction if not repaired promptly. For tendon defects, clinical methods include: (1) autologous tendon transplantation to repair tendon defects; (2) allogeneic tendon transplantation; (3) heterogeneous tendon transplantation; 4) artificial tendon replacement. Due to the lack of donor tendons and immune rejection in the first three, artificial tendons have been widely used clinically with the continuous development of medical implant materials. ...

Claims

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Application Information

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IPC IPC(8): A61L27/36A61L27/56
Inventor 赵博王振军
Owner BEIJING BIOSIS HEALING BIOLOGICAL TECH
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