Biological repair tablet for endocranium and preparation method thereof
A patch and dura mater technology, applied in the field of dura mater biological patch and its preparation, can solve the problems of affecting the anti-infection ability of products, tissue regeneration ability, destroying three-dimensional structure, product instability, etc. Low immunogenicity, high effect to ensure stability and uniformity
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Embodiment 1
[0045] Example 1: Preparation of 8-layer dura mater biological patch
[0046] The preparation method is as follows:
[0047] (1) Determination of animal source and pretreatment and initial washing of small intestine tissue
[0048] The Chinese inbred Wuzhishan minipig was selected as the animal source, and the animal species was determined using the method specified in the patent ZL200510008994.2. The small intestine tissue of the freshly slaughtered Chinese inbred Wuzhishan minipig was cleaned, and the small intestinal submucosa was separated and used Rinse with water for injection 3 times.
[0049] (2) Virus inactivation
[0050] Adopt the peracetic acid-ethanol solution method to inactivate the virus, this step is carried out in the constant temperature ultrasonic cleaner that the cleaning tank can vibrate, wherein the volume percentage of peracetic acid is 0.05~0.2% (preferably 0.1%), inactivation The time is 1 to 2 hours (preferably 1 hour), the temperature range is 4 ...
Embodiment 2
[0059] Embodiment 2: Physical and chemical properties, histology, growth factor and biological performance detection of the material prepared in embodiment 1
[0060] 1. The physical properties of the prepared 8-layer material are tested, and the testing items include porosity measurement, suture retention, tensile strength, and burst strength.
[0061] 1) Porosity measurement: Mercury porosimetry was used to measure the porosity of the material, and compared with and compared with Biodesign Surgisis products. Results: The porosity of the sample provided in Example 1 was 87.8±2.51, and the porosity of the Biodesign Surgisis product was 78.3±6.38.
[0062] 2) Suture retention test: method: use 2-0 surgical sutures or stainless steel wires of the same diameter to suture at 2 mm inside the edge of one end of the patch, and fix the suture or stainless steel wire and the other end of the patch on a tension meter On, stretch at a speed of 20mm / min until the suture point is torn, an...
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