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Artificial biological tendon and preparation method thereof

A biological and tendon technology, applied in the field of artificial biological tendon and its preparation, can solve the problems of affecting the anti-infective ability of the product, tissue regeneration ability, destroying the three-dimensional structure, product instability, etc. High, guaranteed stability and uniformity

Active Publication Date: 2015-04-29
BEIJING BIOSIS HEALING BIOLOGICAL TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

On the other hand, Biodesign Surgisis products use common hybrid pigs as animal sources, and individual differences in the genetic background of hybrid animals lead to instability and inconsistency in different batches of products
On the other hand, the vacuum compression and freeze-drying molding process adopted by Biodesign Surgisis products compresses the spatial structure of SIS materials, destroys the natural three-dimensional structure of ECM, and affects the anti-infection ability of the product and the ability to promote tissue regeneration

Method used

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  • Artificial biological tendon and preparation method thereof
  • Artificial biological tendon and preparation method thereof
  • Artificial biological tendon and preparation method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0042] Embodiment 1: Preparation of 8-layer artificial biological tendon

[0043] The preparation method is as follows:

[0044] (1) Determination of animal source and pretreatment and initial washing of small intestine tissue

[0045] The Chinese inbred Wuzhishan minipig was selected as the animal source, and the animal species was determined using the method specified in the patent ZL200510008994.2. The small intestine tissue of the freshly slaughtered Chinese inbred Wuzhishan minipig was cleaned, and the small intestinal submucosa was separated and used Rinse with water for injection 3 times.

[0046] (2) Virus inactivation

[0047] Adopt the peracetic acid-ethanol solution method to inactivate the virus, this step is carried out in the constant temperature ultrasonic cleaner that the cleaning tank can vibrate, wherein the volume percentage of peracetic acid is 0.05~0.2% (preferably 0.1%), inactivation The time is 1 to 2 hours (preferably 1 hour), the temperature range i...

Embodiment 2

[0056] Embodiment 2: Physical and chemical properties, histology, growth factor and biological performance detection of the material prepared in embodiment 1

[0057] 1. The physical properties of the prepared 8-layer material are tested, and the testing items include porosity measurement, suture retention, tensile strength, and burst strength.

[0058] 1) Porosity measurement: Mercury porosimetry was used to measure the porosity of the material, and compared with and compared with Biodesign Surgisis products. Results: The porosity of the sample provided in Example 1 was 87.8±2.51, and the porosity of the Biodesign Surgisis product was 78.3±6.38.

[0059] 2) Suture retention test: method: use 2-0 surgical suture or stainless steel wire of the same diameter to suture at 2 mm inside the edge of one end of the artificial biological tendon, and fix the suture or stainless steel wire and the other end of the artificial biological tendon on the On the tension meter, stretch at a sp...

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Abstract

The invention provides an artificial biological tendon and a preparation method of the artificial biological tendon. Small intestinal sub mucosa tissues of inbred strain animals are adopted as raw materials of the artificial biological tendon and are removed with cell components and deoxyribonucleic acid (DNA) components, extracellular matrix components and structures are completely reserved, and the artificial biological tendon has a cellular structure. The preparation method of the artificial biological tendon comprises the following operation steps of: determining animal sources, preprocessing small intestine tissues, rough cleaning, viral inactivating, removing cells, clearing DNA, forming, packaging and sterilizing. The inbred strain animals are served as an animal source of the artificial biological tendon prepared by the method and have pure, stable and unite genetic characteristics, the stability and uniformity of different batches of products are basically ensured, the DNA residue of the animal source is small, the three-dimensional structure of the natural extracellular matrix component (ECM) is completely reserved, and the artificial biological tendon has low immunogenicity and strong anti-infection capability.

Description

technical field [0001] The invention relates to the technical field of animal-derived implantable medical devices, in particular to an artificial biological tendon and a preparation method thereof. Background technique [0002] Tendon is a kind of viscoelastic tissue, which can transmit the force generated by muscle to bone and cause the movement of limb. The biological properties of tendons affect the contractility and sports performance of muscles to a certain extent. Tendon damage from exercise or disease often leads to limb dysfunction if not repaired promptly. For tendon defects, clinical methods include: (1) autologous tendon transplantation to repair tendon defects; (2) allogeneic tendon transplantation; (3) heterogeneous tendon transplantation; 4) artificial tendon replacement. Due to the lack of donor tendons and immune rejection in the first three, artificial tendons have been widely used clinically with the continuous development of medical implant materials. A...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61L27/36A61L27/56
Inventor 赵博王振军
Owner BEIJING BIOSIS HEALING BIOLOGICAL TECH
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