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Method for preparing hypotensive substance by using abalone visceral connective tissues

A technology of connective tissue and abalone viscera, which is applied in the field of preparation of antihypertensive substances from the connective tissue of abalone viscera, which can solve the problem of low utilization rate of resources and achieve the effect of improving the effective utilization rate

Active Publication Date: 2013-09-18
陈锦权
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, most of the food and deep processing of abalone are gastropods, and most of the organs accounting for 30-40% of the total weight are discarded, and a very small part is used for processing into animal feed, and the utilization rate of resources is low.

Method used

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  • Method for preparing hypotensive substance by using abalone visceral connective tissues
  • Method for preparing hypotensive substance by using abalone visceral connective tissues
  • Method for preparing hypotensive substance by using abalone visceral connective tissues

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preparation example Construction

[0024] The invention provides a method for preparing abalone visceral antihypertensive substance, the main steps of which include:

[0025] Abalone visceral connective tissue → Soak in 0-4°C, 0.05-0.15mol / l NaOH solution for 5-7 hours → Wash until neutral → Combine pepsin and glacial acetic acid for enzymatic hydrolysis → Crude extract of abalone visceral collagen → 85 Heat pretreatment at ~100°C for 5-15 minutes→enzyme hydrolysis with neutral protease for 5-7 hours→inactivate enzyme in water bath at 85-100°C for 5-15 minutes→centrifuge to remove precipitate→collect supernatant→3~10KD molecular membrane ultrafiltration→take The liquid that passes through the ultrafiltration membrane → flows through the mixed bed of anion and cation exchange resin → concentrate → dry.

Embodiment 1

[0027] A preparation method of abalone viscera antihypertensive substance is as follows:

[0028] (1) Take 1.0 kg of cleaned abalone visceral connective tissue and wash it with NaOH solution (C NaOH =0.05mol / l), soaked at 0°C for 7h, and washed until neutral to obtain product A.

[0029] (2) Add pepsin at 280u / g (based on dry weight of product A) at 25°C, and mix with glacial acetic acid (C 冰醋酸 =0.05mol / l) combined with enzymatic hydrolysis A for 48 hours, the product B was obtained. Under this condition, the collagen extraction rate of the connective tissue of the abalone viscera was 18.35%.

[0030] (3) Heat pretreatment of product B at 85°C for 15 minutes to obtain product C.

[0031] (4) Add neutral protease at 14000u / g (based on the dry weight of product C) at 45°C and pH 7.5, and then enzymatically hydrolyze product C for 7 hours, inactivate the enzyme in a water bath at 85°C for 15 minutes, centrifuge to remove the precipitate, Collect the supernatant to obtain produ...

Embodiment 2

[0034] A preparation method of abalone viscera antihypertensive substance is as follows:

[0035] (1) Take 1.0 kg of cleaned abalone visceral connective tissue and wash it with NaOH solution (C NaOH =0.15mol / l), soaked at 4°C for 5h, and washed until neutral to obtain product A.

[0036] (2) Add pepsin at 200u / g (based on dry weight of product A) at 4°C, and mix with glacial acetic acid (C 冰醋酸 =0.5mol / l) combined with the enzymatic hydrolysis product A for 24h, the product B was obtained. Under this condition, the collagen extraction rate of the abalone visceral connective tissue was 22.28%.

[0037] (3) Heat pretreatment of product B at 100°C for 5 minutes to obtain product C.

[0038] (4) Add AS.1398 neutral protease at 10,000u / g (based on the dry weight of product C) at 55°C and pH 6.5, and then enzymolyze the product C for 5 hours, inactivate the enzyme in a water bath at 100°C for 5 minutes, and centrifuge. Remove the precipitate and collect the supernatant to obtain p...

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Abstract

The invention relates to a method for preparing a hypotensive substance by using abalone visceral connective tissues. The method specifically comprises the following steps: pretreating the abalone visceral connective tissues by using NaOH solution; washing the abalone visceral connective tissues to be neutral; preparing a crude extract of the abalone visceral connective tissues in an acid- enzyme combination mode; preheating the crude extract of the prepared abalone visceral connective tissues; preparing polypeptide by using specific protease; and purifying and drying to prepare an abalone visceral connective tissue hypotensive substance. The abalone visceral connective tissue hypotensive substance provided by the invention is rich in hydrophobic amino acids, proline and the like, and shows high angiotensin converting enzyme (ACE) inhibition rate in vitro, and has a good hypotensive effect in vivo, and can be used for preparing hypotensive adjuvants and amino acid supplements.

Description

technical field [0001] The invention relates to a method for preparing antihypertensive substances from abalone visceral connective tissue. Background technique [0002] Hypertension is an important risk factor for cardiovascular and cerebrovascular diseases such as myocardial infarction, stroke, heart failure and coronary heart disease. my country is a country with high blood pressure. According to the "China Cardiovascular Disease Report 2011", there are nearly 200 million hypertensive patients in my country. However, the adverse effects of long-term increase in blood pressure on cardiovascular and cerebrovascular diseases have greatly affected the health of hypertensive patients. , In the past ten years, the number of patients has increased at a rate of 3.2 million per year. With the continuous improvement of living standards and the continuous acceleration of the pace of society, hypertensive patients are showing a younger trend. Therefore, the prevention and treatment o...

Claims

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Application Information

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IPC IPC(8): C12P21/06
Inventor 陈锦权方婷彭会娟
Owner 陈锦权
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