131i-labeled anti-vegfr2 chimeric Fab and its application
A 131I-FA8H1 and labeling technology, which is applied in the field of biomedicine, can solve the problems of disordered and irregular arrangement of blood vessels, and difficulty in obtaining the effect of anti-tumor drugs, and achieve the goal of inducing apoptosis of liver cancer cells, reducing body immunity, and inhibiting tumor growth Effect
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[0032] 1) FACS: Culture BEL-7402, QCY-7701, SMMC-7721, HepG2 and SK-HEP-1 to the logarithmic growth phase respectively; refer to the above method, detect the difference in the expression level of VEGFR2 in the natural conformation of different liver cancer cell lines by FACS, The FA8H1 concentration was 10 μg / mL. The results showed: SMMC-7721 (67.21%), SK-HEP-1 (72.35%), HepG2 (80.45%), QCY-7701 (84.94%), BEL-7402 (93.73%), among which the highest expression of native conformation VEGFR2 It is the liver cancer cell line BEL-7402.
[0033] 2) Radioactive isotopes 131 I mark FA8H1 (conducted in a fume hood in a professional laboratory):
[0034] Chloramine-T oxidation method (Chloramine-T), the following is the dosage ratio of each reagent: 100μgFA8H1 plus 200μLPB (0.2M, pH8.0); at the same time add 60μL about 74MBqNa 131 I (2.0mCi) was mixed evenly, and then 100μL of the oxidant chloramine-T (3mg / mL) was added, and the reaction was shaken at room temperature for 1min; then, ...
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