131i-labeled anti-vegfr2 chimeric Fab and its application

A 131I-FA8H1 and labeling technology, which is applied in the field of biomedicine, can solve the problems of disordered and irregular arrangement of blood vessels, and difficulty in obtaining the effect of anti-tumor drugs, and achieve the goal of inducing apoptosis of liver cancer cells, reducing body immunity, and inhibiting tumor growth Effect

Inactive Publication Date: 2016-01-20
NANJING MEDICAL UNIV +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

The important reason is that the arrangement of blood vessels in the liver cancer tissue is disordered and irregular, coupled with the high pressure inside the tumor, it is difficult to achieve good results with traditional methods of anti-tumor drugs

Method used

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  • 131i-labeled anti-vegfr2 chimeric Fab and its application
  • 131i-labeled anti-vegfr2 chimeric Fab and its application
  • 131i-labeled anti-vegfr2 chimeric Fab and its application

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Embodiment 1

[0032] 1) FACS: Culture BEL-7402, QCY-7701, SMMC-7721, HepG2 and SK-HEP-1 to the logarithmic growth phase respectively; refer to the above method, detect the difference in the expression level of VEGFR2 in the natural conformation of different liver cancer cell lines by FACS, The FA8H1 concentration was 10 μg / mL. The results showed: SMMC-7721 (67.21%), SK-HEP-1 (72.35%), HepG2 (80.45%), QCY-7701 (84.94%), BEL-7402 (93.73%), among which the highest expression of native conformation VEGFR2 It is the liver cancer cell line BEL-7402.

[0033] 2) Radioactive isotopes 131 I mark FA8H1 (conducted in a fume hood in a professional laboratory):

[0034] Chloramine-T oxidation method (Chloramine-T), the following is the dosage ratio of each reagent: 100μgFA8H1 plus 200μLPB (0.2M, pH8.0); at the same time add 60μL about 74MBqNa 131 I (2.0mCi) was mixed evenly, and then 100μL of the oxidant chloramine-T (3mg / mL) was added, and the reaction was shaken at room temperature for 1min; then, ...

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Abstract

The invention discloses a 131I labeled anti-VEGFR2 chimeric Fab and application thereof. The 131I-labeled anti-VEGFR2 chimeric Fab was prepared by the chloramine-T oxidation method, specifically including: 131I-labeled anti-VEGFR2 chimeric Fab, separation and purification of 131I-FA8H1, determination of radiochemical purity and specific activity. The 131I-labeled anti-VEGFR2 chimeric Fab of the present invention is suitable for radionuclide imaging diagnosis of liver cancer; 131I-FA8H1 can be used for targeted therapy of liver cancer, and 131I-FA8H1 can effectively inhibit tumor growth, which is significantly better than using FA8H1 or 131I alone Effect; 131I-FA8H1 promotes large necrosis of tumor tissue, induces apoptosis of liver cancer cells, and does not inhibit the peripheral blood wbc for a long time to reduce the side effect of immunity of the body. It can be seen that the anti-VEGFR2 chimeric Fab has great potential in the preparation of drugs for the treatment of liver cancer. good application.

Description

technical field [0001] The invention belongs to the technical field of biomedicine, and specifically relates to a 131 I-labeled anti-VEGFR2 chimeric Fab and its application. Background technique [0002] Primary liver cancer (hereinafter referred to as liver cancer) is one of the most common malignant tumors in the world. According to the World Health Organization, there are about 630,000 new cases and 600,000 deaths each year worldwide. Most of the diagnosed liver cancer patients are in Died within a year. my country is one of the areas with a high incidence of liver cancer in the world, and the occurrence of liver cancer accounts for about 55% of all cases in the world. Qidong City in Nantong, Jiangsu Province is a high incidence area of ​​liver cancer in my country. At present, the most effective treatment for liver cancer is early liver cancer surgery. By the time the patient finds symptoms, the liver cancer has entered the middle and late stages, often involving multi...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61K51/10A61P35/00
Inventor 冯振卿黄剑飞朱进咸华咸云唐小军唐奇
Owner NANJING MEDICAL UNIV
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