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Method for evaluating drought resistance of crop seeds during crop seed germination period based on stimulated luminescence

A technology of stimulated light emission and evaluation method, which is applied in the direction of material excitation analysis, seed and rhizome treatment, application, etc., and can solve the problems of long detection method cycle, inaccurate destructive measurement and evaluation, time-consuming and labor-intensive problems, etc.

Inactive Publication Date: 2013-10-09
XIAN UNIV OF TECH
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Problems solved by technology

[0005] The purpose of the present invention is to provide a drought resistance evaluation method of crop seeds based on stimulated light, which solves the problems of long cycle, time-consuming and laborious, destructive measurement and evaluation in existing detection methods

Method used

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  • Method for evaluating drought resistance of crop seeds during crop seed germination period based on stimulated luminescence
  • Method for evaluating drought resistance of crop seeds during crop seed germination period based on stimulated luminescence
  • Method for evaluating drought resistance of crop seeds during crop seed germination period based on stimulated luminescence

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Embodiment

[0106] Zhoumai 18 (weak drought resistance) and Shannong 26 (strong drought resistance) were selected as wheat seeds. Two kinds of wheat seeds with full grain and equal size were treated with 0.2% HgCl 2 Disinfect for 2 minutes, wash and put in a constant temperature incubator at 25°C to cultivate for 24 hours. After the dew-white seeds are selected, 200 seeds with uniform dew-whiteness are selected as a group, and placed evenly in the respective numbered 90mm petri dishes, and a 90mm petri dish is placed at the bottom of the petri dish. Medium-speed filter paper, the seeds are protected from light and kept at a constant temperature (25±0.2°C) during the cultivation process, 3:00, 9:00, 15:00, 21:00 every day, add appropriate amount at four time points, and the osmotic potential is -0.25MPa PEG-6000 culture medium.

[0107] During the seed cultivation process, samples were taken every other day (24h) to measure the excited light of the germinated seeds. The excited light was ...

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Abstract

The invention discloses a method for evaluating drought resistance of crop seeds during a crop seed germination period based on stimulated luminescence. The method comprises the following steps of performing water stress on germinating crop seeds for a few days by a hypertonic solution with a certain concentration by adopting a hypertonic solution method, stimulating the germinating seeds by non-saturated light, instantly measuring light emitted after the germinating seeds are stimulated, and analyzing the stimulated luminescence of the germinating seeds under the water stress to evaluate the drought resistance of the germinating seeds during the seed germination period. According to the method for evaluating the drought resistance of the crop seeds during the crop seed germination period based on the stimulated luminescence, a stimulated luminescence decay curve of the germinating seeds is directly measured by performing the water stress on the germinating seeds only without a field experiment, and the drought resistance of the germinating seeds is nondestructively and quickly evaluated by extracting and analyzing luminous parameters; and the method is simple and practical, and has a reliable evaluation effect.

Description

technical field [0001] The invention belongs to the field of biotechnology, and relates to a method for evaluating plant stress resistance, in particular to a method for evaluating drought resistance of crop seeds in germination stage based on stimulated light. Background technique [0002] Drought is the most important factor affecting crop production. Drought in the germination stage directly affects the germination, growth and development of crop seeds and the formation of yield. Therefore, the drought resistance of crop seeds during germination is one of the most concerned issues in crop drought resistance research. [0003] For a long time, the method of evaluating the drought resistance of crop seeds during germination is to use field method, artificial simulation method or hypertonic solution method to form drought stress, and then judge and evaluate drought resistance based on the biological changes of germinated seeds. The field method is limited by the season, tak...

Claims

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Application Information

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IPC IPC(8): A01C1/00G01N21/63
Inventor 习岗刘锴高宇樊琳琳
Owner XIAN UNIV OF TECH
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