Method for identifying silique shattering resistance of brassica napus
A technology of brassica napus and anti-cracking angle, which is applied in the direction of measuring devices, instruments, and mechanical devices, etc., can solve the problems of inability to effectively distinguish the difference in anti-cracking angle of rapeseed varieties, low accuracy and efficiency of identification results, and identification institutes. It takes a long time to achieve the effect of strong promotion and application value, high identification efficiency and high accuracy
Inactive Publication Date: 2013-10-09
INST OF OIL CROPS RES CHINESE ACAD OF AGRI SCI
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[0006] The invention provides a method for identifying the resistance to cracked horns of Brassica napus, aiming to solve the current method for identifying the cracked horns of Brassica napus, which requires a long time for identification, cumbersome steps, accuracy of identification results and identification efficiency Low, can not effectively distinguish the difference in the resistance of current rapeseed varieties to cracking horns
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[0025] In order to make the objectives, technical solutions, and advantages of the present invention clearer, the following further describes the present invention in detail with reference to the accompanying drawings and embodiments. It should be understood that the specific embodiments described here are only used to explain the present invention, but not used to limit the present invention.
[0026] figure 1 This is the implementation process of the method for identifying the anti-cracking angle of Brassica napus provided by the embodiment of the present invention.
[0027] The method includes the following steps:
[0028] In step S101, the siliques are dried at 80°C for 30 minutes, and after being sealed and stored at room temperature overnight, they are placed in a cylindrical container, and 8 steel balls are placed in the cylindrical container; the siliques are baked at 80°C for 30 minutes to balance moisture,
[0029] In step S102, the HQ45Z shaker is used to oscillate the cyli...
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Abstract
The invention discloses a method for identifying silique shattering resistance of brassica napus. The method comprises: drying a silique at 80 DEG C for 30 min, sealing and reserving at room temperature overnight, putting into a column container in which 8 steel balls are placed; adopting an HQ45Z model shaking table, concussing the column container at a rotary speed of 280 rpm, observing every two minutes for 5 times; and repeating each material for 3 times, and using the formula that silique shattering index = Sigma Xi*(6-i) / silique number*total number to calculate the silique shattering index, while Xi represents the number of broken silique at the i time, 1<=i<=5, and the silique shattering resistance index = 1-silique shattering index. The method is good in operationality, high in accuracy, good in repeatability and high in identification efficiency; and the method helps to effectively discriminate the difference of silique shattering resistance of the present rape varieties, is suitable for popularization and application in screening and identification of rape silique-shattering-resistant variety and heredity research of rape silique shattering resistance, and helps to provide a technology foundation for seed selection and heredity research of rape silique-shattering-resistant variety.
Description
Technical field [0001] The invention belongs to the technical field of identification of resistance to cracking angle of cabbage rape, and particularly relates to a method for identifying the resistance of cracking angle of cabbage rape. Background technique [0002] In the process of crop domestication, in order to prevent crop yield reduction, humans actively choose varieties that reduce cracked corners to make crops more suitable for agricultural production. Rape, soybeans, and sesame, which are the main sources of edible oil raw materials, all have the problem of cracked corners, and rape is more serious. According to the split angle process, the split angle can be divided into active split angle and passive split angle. The active split angle is when the silique matures, the cells lose water and tension is generated between the cells. The cells degrade under the action of enzymes. The tension between the siliques promotes the separation of the siliques from the middle place...
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IPC IPC(8): G01N19/00
Inventor 胡琼彭鹏飞李云昌梅德圣付丽陈玉峰刘佳王会
Owner INST OF OIL CROPS RES CHINESE ACAD OF AGRI SCI