Photoactivated single-molecular fluorescence microscope for biochemical reaction kinetics and test method

Abstract

The invention provides a photoactivated single-molecular fluorescence microscope for biochemical reaction kinetics and a test method. The microscope comprises a sample table, a microscope object lens, an imaging laser transmitter, a camera and an activating laser transmitter. The upper surface of the sample table bears a photopermeable substrate, the sample table is provided with a through hole penetrating the upper and lower surfaces of the sample table, and the photopermeable substrate is covered on the through hole; the upper surface of the photopermeable substrate bears substance molecule A and substance molecule B which undergo a biochemical reaction when mutually contact. The microscope object lens is installed below the photopermeable substrate, and the camera is installed below the microscope object lens. A front lens face of the microscope object lens faces the lower surface of the photopermeable substrate, and an intersection point of the extending line of the principal axis of the microscope object lens and the upper surface of the photopermeable substrate is point o. An imaging laser beam transmitted by the imaging laser transmitter and an activating laser beam transmitted by the activating laser transmitter converge to form one light beam which is incident towards the point o at an incidence angle greater than a critical angle.

Description

technical field [0001] The invention relates to the field of biochemical experiments, in particular to a light-activated microscopic tracking monitoring system. Background technique [0002] In the scientific fields of biology and chemistry, it is of great significance and role to track and monitor biochemical reactions and signal generation processes at the molecular level, because it can avoid the difficulty of synchronizing multiple molecules in the multi-step reaction process and avoid the complexity of a large number of molecules. It is not easy to control the mutual interference, and at the same time, it can obtain more detailed results that cannot be obtained by conventional body detection techniques. Single-molecule fluorescence technology usually uses luminescent molecules / groups to locate and track a single target molecule, and explore detailed information on biochemical processes, so it has a wide range of applications in biology, chemistry, materials and other r...

AI Technical Summary

Problems solved by technology

[0005] However, it is still very difficult to directly observe chemical reactions and protein-protein interactions at the single-molecule level (closely related to the signal generation process in vivo), and to capture their detailed kinetic mechanisms.

Images