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Blood powder enzymolysis and decolorization method

An enzymatic hydrolysis and blood meal technology, applied in the field of enzymatic hydrolysis and decolorization of blood meal, can solve the problems of difficult animal digestion and absorption, no protein degradation, environmental pollution, etc., and achieve the effects of improving palatability, less ring damage, and easy digestion and absorption.

Inactive Publication Date: 2013-10-16
HANGZHOU AIYIN BIOTECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The hemoglobin in the hemoglobin powder is oxidized, and the color is mostly dark red. At the same time, the protein in the hemoglobin powder has not been degraded, and it is not easy to be digested and absorbed by animals as a feed protein raw material, the utilization rate is low, and it causes environmental pollution.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0015] Add 100kg of sprayed blood powder to the reaction tank, add 1000L of water, stir for 12 hours, analyze the pH of the blood powder liquid, adjust the pH to 7.0 with 0.5mol / L NaOH; add 6kg of keratinase (enzyme activity is 30000U / g), and react at 55°C React at high temperature for 4 hours; then add 9kg of neutral protease (enzyme activity is 50000U / g), and react for 5 hours at a reaction temperature of 50°C; measure the pH of the reaction solution, adjust the pH to 4.0 with 0.5mol / L HCl, and inhibit the enzyme Live, terminate the reaction, centrifuge at 5000r / min for 10min, discard the residue; add activated carbon at 0.2 g / 100ml to the supernatant, react at 55°C, pH 4.0, decolorize for 50min, discard the residue by centrifugation, and obtain the decolorization reaction solution . Experiments have proved that in this embodiment, the degradation rate of hemoglobin is as high as 92%.

Embodiment 2

[0017] Add 100kg of sprayed blood powder to the reaction tank, add 1000L of water, stir for 12 hours, analyze the pH of the blood powder liquid, adjust the pH to 7.0 with 0.5mol / L NaOH; add 7kg of keratinase (enzyme activity is 30000U / g), and react at 55°C React at high temperature for 4 hours; then add 8kg of neutral protease (enzyme activity is 50000U / g), and react for 5 hours at a reaction temperature of 50°C; measure the pH of the reaction solution, adjust the pH to 4.0 with 0.5mol / L HCl, and inhibit the enzyme Live, terminate the reaction, centrifuge at 5000r / min for 10min, discard the residue; add 0.2 g / 100ml of activated carbon to the supernatant, react at 55°C, pH 4.0, decolorize for 50min, and discard the residue by centrifugation to obtain the decolorization reaction solution . Experiments have shown that in this embodiment, the degradation rate of hemoglobin is as high as 93%.

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Abstract

The invention discloses a blood powder enzymolysis and decolorization method which comprises the steps that spraying blood powder is taken as a raw material; water is added to the blood powder for dissolution for 12h; the pH is adjusted to be 7.0 with 0.5mol / L NaOH; keratinase is added according to the quantity of 1800-2100u / g substrate; a reaction is conducted for 4h at a reaction temperature of 55 DEG C; neutral protease is added according to the quantity of 4000-5000u / g substrate; a reaction is conducted for 5h at a reaction temperature of 55 DEG C; the pH of a reaction liquid is adjusted to be 4.0 with 0.5mol / L HCl; the enzyme activity is inhibited to terminate the reaction; centrifugation is conducted for 10min at 5000r / min; residues are discarded; activated carbon is added to a supernate according to a mass volume ratio of 0.2g / 100ml; the reaction temperature is 55 DEG C; the pH is 4.0; decolorization is conducted for 50min; the centrifugation is conducted; the residues are discarded; and the decolorized reaction liquid is formed. According to the method, enzymolysis liquid is safe; an enzymolysis rate is high; and developed albumen powder is high in digestion and utilization rate.

Description

technical field [0001] The invention relates to a process for treating livestock and poultry blood, in particular to a method for enzymatic hydrolysis and decolorization of blood meal. Background technique [0002] my country's livestock and poultry blood is rich in resources and comprehensive in nutrition. Its protein content is as high as 18% to 22%, and it also contains eight kinds of essential amino acids for the human body, of which lysine content is as high as 14%, which is an ideal source of protein nutrition. Due to the color and fishy smell of hemoglobin, adding a small amount in food and feed will also make food and feed dark brown and have peculiar smell, which will affect the sensory quality and limit its wide application. Although the total amount of pig blood in China is quite abundant, it has a vast area and is relatively scattered, which is not conducive to collection and post-processing, which limits the development and production of pig blood products. The...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A23K1/04A23K10/24
Inventor 来钢云
Owner HANGZHOU AIYIN BIOTECH
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