Method for extracting erythritol from erythritol mother liquor and special barm strain for erythritol

An erythritol and yeast technology, applied in chemical instruments and methods, preparation of organic compounds, organic chemistry, etc., can solve problems such as waste of resources and damage to the environment, and achieve short purification time, environmental friendliness, and high extraction efficiency. Effect

Active Publication Date: 2013-10-30
SHANGHAI JIAO TONG UNIV +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Therefore, most erythritol production enterprises in my country use erythritol mother liquor as waste

Method used

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  • Method for extracting erythritol from erythritol mother liquor and special barm strain for erythritol
  • Method for extracting erythritol from erythritol mother liquor and special barm strain for erythritol
  • Method for extracting erythritol from erythritol mother liquor and special barm strain for erythritol

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0040] Embodiment 1, the separation and domestication of yeast strain (Candida sp.)

[0041] The 350 kinds of yeast preserved in the laboratory were streaked and inoculated on the basic medium containing glycerol, mannitol, arabitol, ribitol, erythritol and maltose respectively (basic medium composition: yeast nitrogen base 0.67 g / liter, agar 2 g / liter), cultivated at 30°C for 5 days, and observed the growth of various bacterial strains on the above-mentioned medium.

[0042] It was found that a strain could grow well on the basic medium containing glycerol, mannitol, arabitol, ribitol and maltose, but could not grow on the basic medium containing erythritol.

[0043] Molecular identification of 26S rDNA gene was carried out on the yeast strain. Universal primers for amplifying yeast 26S rDNA gene can be obtained in relevant public databases (such as NCBI database), and methods for extracting yeast DNA can also be obtained through public databases (such as Springer and Els...

Embodiment 2

[0048] Embodiment 2, the HPLC analysis and component analysis of erythritol mother liquor

[0049] Dilute the erythritol mother liquor 20 times with deionized water and pass it through a 0.2 micron filter membrane, take 50 microliters for HPLC analysis, the chromatographic conditions used are: temperature 70°C, mobile phase is deionized water, flow rate is 1.0ml / min. The analytical chromatographic column used is a Shodex SP0810 type analytical column, and the detector is a differential refractive index detector. The HPLC analytical spectrum of erythritol mother liquor is as follows Figure 4 As shown in A, the peak substance at 7.3min is maltoligosaccharide. The peaks with peak times of 13.7min and 18.8min were collected respectively, freeze-dried, dissolved in water for high-efficiency thin-layer analysis, and compared with various polyol standard products. The schematic diagrams of component analysis and identification are as follows Figure 4 B. Figure 4 As shown in...

Embodiment 3

[0050] Embodiment 3, yeast strain (Candida sp.SJTU828) CGMCC No.7323 sends out in 250ml shaking flask Yeast purification erythritol mother liquor

[0051] Prepare a fermentation medium, the composition of which is: dilute the erythritol mother liquor by 1 time with tap water (that is, add an equal volume of water), add yeast powder with a mass volume percentage of 3.0%, adjust the pH to 5.0, and sterilize. After cooling, insert the bacterial strain (Candida sp.SJTU828) CGMCC No.7323 into a 250ml shake flask containing 20ml fermentation medium (in order to increase dissolved oxygen, add a wire ring at the bottom of the shake flask), at 25°C, 250 rpm conditions for fermentation purification. Samples were regularly sampled for HPLC analysis, and the results are shown in Figure 8, wherein the substance with a retention time of 7.3min is maltooligosaccharide, the substance with a retention time of 13.7min is erythritol, and the substance with a retention time of 18.8min is arab...

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Abstract

The invention discloses a method for extracting erythritol from an erythritol mother liquor and a special barm strain for the erythritol. The method comprises the following step of adding the barm strain into the erythritol mother liquor for fermentation and purification, wherein the barm strain can be used for catabolism of polyhydric alcohols and saccharides in the erythritol mother liquor except the erythritol. The special barm strain is a candida (Candida sp. SJTU828) CGMCC NO.7323. The erythritol is extracted from the erythritol mother liquor through an industrialization method of biological fermentation and purification, the extraction efficiency is high, the method is friendly to environment, and the waste resource is economized.

Description

technical field [0001] The invention belongs to the field of biotechnology, and in particular relates to a method for extracting erythritol from erythritol mother liquor and special yeast strains thereof. Background technique [0002] Erythritol is a low-calorie and sweet functional sugar alcohol, which is widely used in the food field. Erythritol is mainly synthesized by yeast fermentation of glucose. In the process of fermenting glucose to synthesize erythritol, different yeasts will produce other different kinds of polyols in addition to the target product of erythritol. For example, Candida lipolytica (now called Yarrowia lipolytica), which is widely used by erythritol enterprises in my country, not only synthesizes erythritol, but also produces a certain amount of glycerin, ribitol, arabitol and mannose. Alcohol by-products (Tokuoka K, Ishitani T, Chung WC. 1992. J Gen Appl Microbiol 38:35). Candida magnolia produces a glycerol by-product in addition to erythritol syn...

Claims

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Application Information

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IPC IPC(8): C07C31/24C07C29/88
Inventor 程海荣吕霁烊王犇邓子新
Owner SHANGHAI JIAO TONG UNIV
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