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Preparation method for living hypertension animal model caused by high-purine feed feeding

An animal model, high purine technology, applied in the field of medicine, can solve problems such as animal models that have not been seen

Inactive Publication Date: 2013-11-13
ZHEJIANG CHINESE MEDICAL UNIVERSITY
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  • Claims
  • Application Information

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Problems solved by technology

At present, the common hypertensive rat models in the laboratory include spontaneously hypertensive rats, two-kidney-one-clip hypertensive rat models, etc., but no animal models close to the pathogenesis of human hypertension have been seen. The present invention is based on daily life , the cause of human hypertension, such as long-term use of high-purine food, established an animal model that can be used for the screening, pharmacological research and drug efficacy evaluation of drugs for the treatment of active hypertension

Method used

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  • Preparation method for living hypertension animal model caused by high-purine feed feeding
  • Preparation method for living hypertension animal model caused by high-purine feed feeding
  • Preparation method for living hypertension animal model caused by high-purine feed feeding

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Experimental program
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Effect test

Embodiment 1

[0007] 1 Experimental materials and instruments

[0008] 1.1 Animals SD rats, half male and half male, weighing 180-220 g, 20 rats. License No. SCXK (Zhejiang) 20080033, provided by Zhejiang Experimental Animal Center.

[0009] instrument ALC-NIBP non-invasive blood pressure measurement and analysis system (Shanghai Alcott Biotechnology Co., Ltd.); Holland Ritu SELECTRA-E automatic biochemical analyzer (Netherlands Ritu company); Powerwave340 microplate reader (US Bio-TEK company); DH -500K / Na / Cl / Ca / pH electrolyte analyzer (Shenzhen Baikangli Technology Co., Ltd.).

[0010] Reagent Alanine aminotransferase, aspartate aminotransferase, γ-glutamyl transpeptidase, alkaline phosphatase, uric acid, creatinine (Shanghai Fosun Long March Medical Science Co., Ltd.); BUN kit, Nanjing Jiancheng Bioengineering Institute; A Calibration / Washing Solution, B Slope Calibration Solution, Protein-Removing Cleaning Solution, manufactured by Wuhan Zhongda Biosensing Technology Co., L...

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Abstract

The invention belongs to the technical field of medicine, and particularly relates to a preparation method for a living hypertension animal model caused by high-purine feed feeding. The model can be used for the screening, pharmacological studies and efficacy evaluation of medicaments for treating living hypertension. The living hypertension animal model can be established by feeding high-purine feeds to normal SD rats every day for 8 to 17 weeks. The influence of the long-term feeding of the high-purine feeds on the blood pressure of the rats and functions of visceral organs such as the liver and the kidney is monitored by taking blood pressure indexes, mainly systolic pressure, as main model screening indexes and taking indexes in serum and urine as auxiliary indexes.

Description

technical field [0001] The invention belongs to the technical field of medicine, and in particular relates to a method for preparing an animal model of active hypertension induced by high-purine feed feeding. The model can be used for screening, pharmacological research and drug effect evaluation of drugs for treating active hypertension. Background technique [0002] With the improvement of people's living standards and changes in dietary structure, hyperuricemia is becoming more and more common. Foreign studies have found that basal blood uric acid level is the strongest independent predictor of hypertension. At the same time, hyperuricemia can lead to uric acid nephropathy. The kidney is directly involved in the occurrence of essential hypertension through the regulation of body fluids and the production of vasoactive substances. Hypertension is an important independent risk factor for kidney damage, increasing the incidence of kidney disease and the incidence and morta...

Claims

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Application Information

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IPC IPC(8): A01K67/027
Inventor 吕圭源陈素红苏洁夏超群
Owner ZHEJIANG CHINESE MEDICAL UNIVERSITY
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