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Separated oligonucleotide rno-miR-181a and application thereof in brain mitochondria damage

An oligonucleotide and mitochondrial technology, applied in the field of molecular biology, can solve problems such as blank research

Active Publication Date: 2013-11-20
INST OF RADIATION MEDICINE ACAD OF MILITARY MEDICAL SCI OF THE PLA
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] However, research on biomarkers of brain mitochondrial damage caused by microwave radiation is rare, especially the research on miRNA markers is still blank

Method used

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  • Separated oligonucleotide rno-miR-181a and application thereof in brain mitochondria damage
  • Separated oligonucleotide rno-miR-181a and application thereof in brain mitochondria damage
  • Separated oligonucleotide rno-miR-181a and application thereof in brain mitochondria damage

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0036] Embodiment 1 carries out microwave radiation to rat

[0037] 56 secondary male Wistar rats (Academy of Military Medical Sciences) with a body weight of 220±20 g were randomly divided into a sham radiation group and a microwave radiation group. The whole body of the above-mentioned rats was uniformly irradiated by the microwave radiation source of the Academy of Military Medical Sciences, and the average power density of the microwave radiation source was 30mW / cm 2 , radiation once every other day, a total of 3 times, 10 minutes each time. The sham radiation group was placed in a radiation box without radiation.

Embodiment 2

[0038] Embodiment 2 Observation of rat brain tissue structure

[0039]Rats in the radiation group and the sham radiation group were treated with intraperitoneal injection of 1% pentobarbital sodium (30 mg / kg) on ​​the 1st day, 7th day, 14th day and 28th day after the normal feeding was resumed respectively. After anesthesia, the brain tissue was taken and fixed with 10% buffered formalin for 1 week, dehydrated with graded ethanol, transparent in xylene, embedded in paraffin, sectioned at a thickness of 3 microns, deparaffinized to distilled water, stained with Harris hematoxylin, 70 % hydrochloric acid ethanol differentiation, 1% ethanol eosin staining, gradient ethanol dehydration, xylene transparent, neutral gum mounting, light microscope observation and photography. see results figure 1 .

[0040] The brain tissue of the rats in the sham radiation group showed a normal shape and structure, with clear outlines of neurons, mostly round, large and round nuclei, lighter color...

Embodiment 3

[0041] Example 3 Observation of Ultrastructure of Rat Brain Mitochondria

[0042] The rats in the radiation group and the sham radiation group were sacrificed on the 7th day after the resumption of feeding after radiation, and the fresh brain tissue was separated, and a 1mm 3 Brain tissue was quickly fixed in 2.5% glutaraldehyde for 2 hours, then fixed in 1% osmic acid for 2 hours, dehydrated in gradient ethanol and acetone, embedded in Epon812 resin, and after positioning the semi-thin section, make ultra-thin sections (thickness 70 nm) , double-stained with uranyl acetate and lead citrate, observed with a transmission electron microscope and photographed. see results figure 2 .

[0043] Depend on figure 2 The results showed that the cytoplasm of brain neurons in the sham radiation group was rich in mitochondria, and its structure was complete. On the 7th day after resuming routine feeding after radiation, the mitochondria of brain neurons were enlarged, mainly showing ...

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PUM

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Abstract

The invention provides a separated oligonucleotide as well as a kit and a system for determining the existence of brain damage in a biological sample. The separated oligonucleotide has a sequence as shown in SEQ ID NO: 1. The kit and the system provided by the invention can be used for effectively screening whether the brain mitochondria damage exists in the biological sample.

Description

technical field [0001] The invention relates to the field of molecular biology, in particular, the invention relates to an isolated oligonucleotide, a kit for determining the presence of brain mitochondrial damage in biological samples and a system for determining the presence of brain mitochondrial damage in biological samples. Background technique [0002] The biological hazard of microwave radiation has attracted more and more attention from scholars at home and abroad. Studies have confirmed that the brain is a sensitive target organ of microwave radiation. Neurons are extremely sensitive to energy reduction due to their functional specificity. The structure and function of mitochondria are closely related to energy metabolism, and they are also one of the earliest targets involved in microwave radiation. Studies have shown that 30mW / cm 2 Microwave radiation can cause damage to brain tissue structure, mitochondrial structure damage and dysfunction in rats. The above-...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N15/113C12Q1/68C12M1/34
Inventor 赵黎彭瑞云孙成峰熊璐高亚兵董霁姚斌伟张静
Owner INST OF RADIATION MEDICINE ACAD OF MILITARY MEDICAL SCI OF THE PLA