Air plant tissue culture medium and method for tissue cultivating and quickly propagating air plant

A tissue culture medium and air pineapple technology, applied in the field of plant cultivation, can solve problems such as slow reproduction speed and the like

Inactive Publication Date: 2013-12-25
JIANGSU POLYTECHNIC COLLEGE OF AGRI & FORESTRY
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Although conventional seed propagation has a high reproduction coefficient, its reproduction rate is extremely slow

Method used

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  • Air plant tissue culture medium and method for tissue cultivating and quickly propagating air plant
  • Air plant tissue culture medium and method for tissue cultivating and quickly propagating air plant
  • Air plant tissue culture medium and method for tissue cultivating and quickly propagating air plant

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Embodiment 1

[0014] Embodiment 1: a kind of air pineapple tissue culture medium, the formula of described culture medium is as follows: 1 / 2MS+IAA0.5mg L -1 +3% sucrose+0.6% agar, pH5.6~5.8. Among them, 3% sucrose means adding 30g sucrose to 1L medium; 0.6% agar means adding 6g agar powder to 1L medium.

[0015] Utilize the method for tissue culture of the above-mentioned Tillandsia twilight tissue culture medium to rapidly propagate Tillandsia twigs, the method comprises the following steps:

[0016] 1) Select air pineapple pods as explants;

[0017] 2) Disinfect the explants;

[0018] 3) Sowing: Pour the sterilized explants on sterile paper and spread them flat, take sterile absorbent paper and spread them on the explants to fully absorb the moisture on the surface of the explants; then use sterilized scissors and Cut the explants with tweezers, put the seeds in the explants into the prepared medium; culture conditions: temperature 22-28°C, 12 hours of light per day, and light intensit...

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Abstract

The invention discloses an air plant tissue culture medium. The formula of the culture medium comprises the following components: 1/2 MS, 0.5mg.L <-1> of IAA, 3% of cane sugar and 0.6 % of agar, wherein the pH value is 5.6-5.8. After the culture by adopting the culture medium provided by the invention, the air plant seed propagation speed is increased, and the growth speed of the air plant is further increased based on the improved air plant propagation coefficient; seeds of the air plant can be taken out from a bottle to be planted after being subjected to tissue culture and then being cultivated in a culture room for about 9 months; in the tissue culture and sowing, a seedling grows into two true leaves for 1 month, 3-4 true leaves for 2 months, and 6-8 true leaves for 3 months, so that the growth speed is improved by 300 % above compared with that under the natural state.

Description

technical field [0001] The invention belongs to the technical field of plant cultivation, and in particular relates to a tissue culture medium of twig pineapple and a method for tissue culture and rapid propagation of twig pineapple. Background technique [0002] Air pineapple (scientific name Tillandsia, English name Air plant) is a perennial herb of Bromeliaceae (Tillandsia), including nearly 550 varieties and 90 varieties, with various varieties and shapes. The varieties selected in this experiment are T. stricta among the bushy varieties. Air pineapple has a special function of purifying indoor air, but there are relatively few provenances of air pineapple in my country, and measures should be taken to speed up its reproduction. [0003] The propagation of Tillandsia mainly depends on seed propagation and branch reproduction. The two varieties selected in this test can produce a large number of fruit through artificial pollination. One plant can have 5-10 pods, and one ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01H4/00
Inventor 郑凯丁久玲史青云蔡鸿宇朱艳
Owner JIANGSU POLYTECHNIC COLLEGE OF AGRI & FORESTRY
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