Method for comprehensively extracting dihydromyricetin and polysaccharide in vine tea at low temperature
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A dihydromyricetin, comprehensive extraction technology, applied in the direction of organic chemistry, to achieve the effect of good color, lower extraction temperature, and shorten extraction time
Inactive Publication Date: 2013-12-25
JIANGXI DONG NAN WILD PLANT DEV
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[0007] The purpose of the present invention is to solve the problems existing in the extraction of polysaccharides from vine tea and dihydromyricetin. Method for comprehensively extracting polysaccharides and dihydromyricetin in rattan tea at relatively low temperature
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Embodiment 1
[0023] Example 2:
[0024] (1) Fresh vine tea stems and leaves are fully dried, then crushed until they pass through a 40-mesh sieve, and 100g is accurately weighed.
[0025] (2) Add 3L of water at 50°C, stir for 3-5min under heat preservation to disperse the material evenly in the water, then turn on the ultrasonic wave, the ultrasonic power is 800W, after ultrasonication for 20min, let the material mixture cool naturally, then place it at 4°C for 12- After 24 hours, the flavonoids (mainly composed of dihydromyricetin) in the solution were crystallized.
[0026] (3) The mixture was centrifuged, and the supernatant was the extract of vine tea polysaccharides, concentrated by rotary evaporation to 500mL-600mL, added 3 times of absolute ethanol to precipitate, stood at 4°C for 4 hours, then suction filtered and washed with absolute ethanol, After the filter residue is dried, the crude polysaccharide of vine tea is obtained, and the yield is about 5%-7%.
Embodiment 2
[0028] Example 3
[0029] (1) Fresh vine tea stems and leaves are fully dried, then crushed until they pass through a 40-mesh sieve, and 100g is accurately weighed.
[0030] (2) Add 3L of water at 60°C, stir for 3-5 minutes under heat preservation to disperse the material evenly in the water, and then turn on the ultrasonic wave with an ultrasonic power of 600W. After 24 hours, the flavonoids (mainly composed of dihydromyricetin) in the solution were crystallized.
[0031] (3) The mixture was centrifuged, and the supernatant was the extract of vine tea polysaccharides, concentrated by rotary evaporation to 500mL-600mL, added 4 times of 95% ethanol for precipitation, left standing at 4°C for 4h, suction filtered and washed with absolute ethanol, After the filter residue is dried, the crude polysaccharide of vine tea is obtained, and the yield is about 5%-8%.
Embodiment 3
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Abstract
The invention provides a method for extracting dihydromyricetin and polysaccharide in vine tea at a relatively low temperature of 40-60 DEG C. The method for extracting dihydromyricetin and polysaccharide in vine tea is realized through the following technological means and technological processes: drying stems and leaves of vine tea, grinding, sieving, carrying out treatment for a certain time at a certain temperature and a certain ultrasonic power while water is taken as a solvent, carrying out natural cooling, then standing for 12-24 hours to crystallize and precipitate dihydromyricetin, centrifuging a feed liquid mixture, concentrating the obtained supernate, carrying out ethanol precipitation, washing and drying, so as to obtain crude polysaccharide of the vine tea; adding boiling water into residue after centrifugation, stirring, and insulating for about 10 minutes so as to ensure that crystallized dihydromyricetin is redissolved, then centrifuging immediately, and after the supernate is cooled, carrying out standing for 12-24 hours at 4 DEG C so as to ensure that dihydromyricetin is crystallized; centrifuging, and drying precipitate, so that dihydromyricetin crude extract is obtained. By adopting the method for extracting dihydromyricetin and polysaccharide in vine tea, the solubilizing effect of ultrasonic wave and the cell disruption effect are utilized for realizing rapid, efficient and synchronous extraction of two active substances in the vine tea at a low temperature, the yield of the dihydromyricetin and polysaccharide products is high, properties of the crude extract are good, and subsequent purification steps are effectively reduced, so that the energy consumption and the cost are reduced.
Description
[0001] technical field [0002] The invention relates to a method for comprehensively extracting polysaccharides and dihydromyricetin in rattan tea at low temperature, and belongs to the technical field of extraction of active ingredients of traditional Chinese medicines. Background technique [0003] Vine tea, commonly known as mildew tea, vine po tea, dragon beard tea, etc. Ampelopsis grossedentata ), widely distributed in the southern provinces of my country. Since ancient times, folks have used rattan tea to treat colds, fever, sore throat, jaundice hepatitis and other diseases. Modern pharmacological studies have shown that the flavonoids in vine tea (mainly composed of dihydromyricetin) have anti-oxidation, anti-tumor, anti-virus, anti-inflammatory and analgesic, broad-spectrum antibacterial, hypoglycemic, hypolipidemic, and liver-protecting effects. The polysaccharides in vine tea have various biological activities such as immune regulation, anti-oxidation, anti...
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