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Electrochemical immunodetection method for detecting estrols

An immunological detection method and electrochemical technology, applied in the field of electrochemical immunological detection based on antibody cross-reaction, can solve the problems of cumbersome process operation and inability to realize on-site detection, etc., and achieve the effect of low detection limit

Inactive Publication Date: 2013-12-25
NANJING NORMAL UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, these analysis methods require large-scale analysis instruments, the process operation is cumbersome, and on-site detection cannot be realized. It is necessary to study and establish more efficient, fast and sensitive analysis and test methods

Method used

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  • Electrochemical immunodetection method for detecting estrols
  • Electrochemical immunodetection method for detecting estrols
  • Electrochemical immunodetection method for detecting estrols

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0027] Example 1 Detection of diethylstilbestrol by electrochemical immunoassay method

[0028] Preparation of electrochemical immunosensor:

[0029] A glassy carbon electrode with a diameter of 3 mm was sequentially filled with Al with a diameter of 0.3 μm and 0.05 μm 2 o 3 Grinding with polishing powder, followed by ultrasonic cleaning with absolute ethanol-distilled water and distilled water for 5 minutes, and then rinsed with distilled water. First deposit nano-gold on the cleaned electrode by electrochemical deposition, then drop-coat 4 μL of graphene-chitosan dispersion on the electrode, and then drop-coat 2 μL of 0.2 mg / mL diethylstilbestrol solution on the electrode surface, 40 Dry at ℃. The modified electrode was soaked in 5% BSA solution and incubated in a 37 °C oven for 30 min to block the remaining active sites.

[0030] The electrochemical immunosensor modified by nano-gold / diethylstilbestrol / graphene / chitosan composite was immersed in a total volume of 50 μL ...

Embodiment 2

[0033] Example 2 Detection of Hexestrol by Electrochemical Immunoassay Method

[0034] Preparation of electrochemical immunosensor:

[0035] A glassy carbon electrode with a diameter of 3 mm was sequentially filled with Al with a diameter of 0.3 μm and 0.05 μm 2 o 3Grinding with polishing powder, followed by ultrasonic cleaning with absolute ethanol-distilled water and distilled water for 5 minutes, and then rinsed with distilled water. First deposit nano-gold on the cleaned electrode by electrochemical deposition, then drop-coat 4μL graphene-chitosan dispersion on the electrode, and then drop-coat 2μL 0.2mg / mL hexestrol solution on the electrode Surface, dry at 40°C. The modified electrode was soaked in 5% BSA solution and incubated in a 37 °C oven for 30 min to block the remaining active sites.

[0036] The electrochemical immunosensor modified by nano-gold / hexestrol / graphene / chitosan composite was soaked in a total volume of 50 μL containing hexestrol antibody with a po...

Embodiment 3

[0039] Example 3 Detection of bisphenol A by electrochemical immunoassay method

[0040] Preparation of electrochemical immunosensor:

[0041] A glassy carbon electrode with a diameter of 3 mm was sequentially filled with Al with a diameter of 0.3 μm and 0.05 μm 2 o 3 Grinding with polishing powder, followed by ultrasonic cleaning with absolute ethanol-distilled water and distilled water for 5 minutes, and then rinsed with distilled water. First deposit nano-gold on the cleaned electrode by electrochemical deposition, then drop-coat 4μL graphene-chitosan dispersion on the electrode, and then drop-coat 2μL 0.2mg / mL bisphenol A solution on the surface of the electrode , dried at 40°C. The modified electrode was soaked in 5% BSA solution and incubated in a 37 °C oven for 30 min to block the remaining active sites.

[0042] The electrochemical immunosensor modified by nano-gold / bisphenol A / graphene / chitosan composite was immersed in a total volume of 50 μL containing diethylst...

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Abstract

The invention discloses an electrochemical immunodetection method for detecting estrols comprising three artificial synthesized compounds possessing estrogen-like effects, and the three compounds are diethylstilbestrol, hexestrol and bisphenol A. The detection method comprises: preparing an electrochemical sensor which has deposited nano-gold and is modified by a graphene / estrol / chitosan compound, then taking K3[Fe(CN)6] as a probe, based on the cross immunity reaction of diethylstibestrol antibody to diethylstilbestrol, hexestrol and bisphenol A, achieving the detection on the three estrols. According to the method provided by the invention, the detection limit of diethylstibestrol is 0.1 ng / mL, linearity range is 1-2500 ng / mL; the detection limit of hexestrol is 0.3 ng / mL, linearity range is 1-3000 ng / mL; and the detection limit of bisphenol A is 0.2 ng / mL, linearity range is 1-3000 ng / mL.

Description

technical field [0001] The invention belongs to the technical field of food safety detection and analytical chemistry, and relates to a detection method capable of detecting three artificially synthesized compounds with estrogen-like effects, namely diethylstilbestrol, hexestrol and bisphenol A (hereinafter referred to as estrol) , specifically an electrochemical immunoassay based on antibody cross-reactivity. Background technique [0002] Diethylstilbestrol (DES) and hexestrol (HEX) (see formula 1 for structure) belong to 1,2-stilbene drugs, which are synthetic estrogens. Stilbene hormones can promote animal growth, increase feed conversion rate, and reduce fat synthesis. In the 1960s, they were officially approved as growth-promoting agents by various countries for animal husbandry production. However, the abuse of 1, 2-stilbene drugs can lead to dysfunction of animal physiological systems, and even irreversible lesions, and eventually endanger human health through the fo...

Claims

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Application Information

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IPC IPC(8): G01N27/416G01N33/53
Inventor 邵科峰王传现赵波张芹吴珺
Owner NANJING NORMAL UNIVERSITY
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