Method and kit for establishing experimental model of diabetic foot ulcer big mouse infected by staphylococcus aureus
What is Al technical title?
Al technical title is built by PatSnap Al team. It summarizes the technical point description of the patent document.
A technique for diabetic foot ulcer and staphylococcal infection, applied in the field of establishing experimental model of diabetic foot ulcer rats infected by Staphylococcus aureus
Inactive Publication Date: 2014-01-01
SHUGUANG HOSPITAL AFFILIATED WITH SHANGHAI UNIV OF T C M
View PDF1 Cites 5 Cited by
Summary
Abstract
Description
Claims
Application Information
AI Technical Summary
This helps you quickly interpret patents by identifying the three key elements:
Problems solved by technology
Method used
Benefits of technology
Problems solved by technology
The results of literature research show that the existing animal models of diabetic foot ulcers mostly simulate a single pathogenic factor (such as peripheral arterial disease, neuropathy or infection), and t
Method used
the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more
Image
Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
Click on the blue label to locate the original text in one second.
Reading with bidirectional positioning of images and text.
Smart Image
Examples
Experimental program
Comparison scheme
Effect test
Embodiment 1
[0023] Rats with type 2 diabetes mellitus induced by high-fat feed combined with streptozotocin, using a scald apparatus to make deep second-degree burns to induce skin ulcers, and using subcutaneous injection of quality control bacteria Staphylococcus aureus-ATCC25923 (Staphylococcus aureus) strain to make A rat model of diabetic foot ulcer infection.
[0024] 1 Materials and methods
[0025] 1.1 Animals: SD male rats, SPF grade, body weight (180±10) g, provided by the Experimental Animal Center of Shanghai University of Traditional Chinese Medicine.
[0026] 1.2 Main reagents and drugs: Streptozotocin (STZ), purchased from Alexis Company; high-fat feed (ingredients: basic feed 73%, lard 20%, milk powder 2%, cholesterol 1%, sucrose 4%; The basic feed formula: 15% barley, 31% wheat, 15% corn, 15% bran, 6% fishmeal, 18% soybean meal, oil and salt, the basic feed was purchased from the Naval Medical Research Institute of the Chinese People’s Liberation Army), high The preparat...
Embodiment 2
[0039] Example 2 Western medicine-recombinant bovine basic fibroblast growth factor for external use (trade name: Beifuji) intervention test results
[0040] 1. Methods: Diabetic control group (DM) without treatment; normal ulcer group (N), diabetic ulcer group (DU), S. aureus control group (SD-C), normal saline dressing change every day; S. aureus treatment group ( SD-T), Beifuji 800iu wound dressing was changed daily. Measure 0d, 3d, 10d after dressing change, wound area after healing, 3% pentobarbital sodium in 2ml Kg after healing -1 The dose was anesthetized by intraperitoneal injection, blood was collected from the abdominal aorta, serum was separated by centrifugation, and skin tissue was taken for examination.
[0041] 2. Data processing: SPSS16.0 was used for statistical analysis, and measurement data was used Indicates that the data conforming to normality and homogeneity of variance were analyzed by one-way analysis of variance, and the components were tested b...
the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more
PUM
Login to view more
Abstract
The invention discloses a method for establishing an experimental model of a diabetic foot ulcer big mouse infected by staphylococcus aureus. The method includes the following steps that after the big mouse is fed on high-fat diet for eight weeks, streptozotocin is injected, and a diabetic model is manufactured. After the diabetic model is stabilized, II-degree scalding is performed on the skin of the big mouse, and after three days, subcutaneous injection of suspension liquid with staphylococcus aureus as the bacterial strain is performed in each ulcer position. In addition, the invention further discloses a kit for establishing the experimental model of the diabetic foot ulcer big mouse infected by the staphylococcus aureus, wherein the kit comprises the staphylococcus aureus suspension liquid. The diabetic foot ulcer experimental animal model detects variation of biochemical indexes after pharmacological intervention, and a result shows that the model has the infection features of diabetic foot ulcer and concurrent gram-positive bacteria. The model has the advantages of being short in ulcer wound surface healing time, sensitive to drug reaction and the like.
Description
technical field [0001] The invention belongs to the field of experimental animal models, and in particular relates to a method and a kit for establishing an experimental model of diabetic foot ulcer rats infected by Staphylococcus aureus. Background technique [0002] With the sharp increase in the incidence of diabetes in my country, the number of patients with diabetic ulcers has also increased. Establishing a disease model that truly simulates diabetic ulcers at the overall animal level is an important tool for medical research such as exploring the pathogenesis of diabetic ulcers and preclinical pharmacodynamic evaluation. It has very important scientific and clinical significance. [0003] At present, animal models of diabetic foot ulcers are mostly induced animal models. The results of literature research show that the existing animal models of diabetic foot ulcers mostly simulate a single pathogenic factor (such as peripheral arterial disease, neuropathy or infection)...
Claims
the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more
Application Information
Patent Timeline
Application Date:The date an application was filed.
Publication Date:The date a patent or application was officially published.
First Publication Date:The earliest publication date of a patent with the same application number.
Issue Date:Publication date of the patent grant document.
PCT Entry Date:The Entry date of PCT National Phase.
Estimated Expiry Date:The statutory expiry date of a patent right according to the Patent Law, and it is the longest term of protection that the patent right can achieve without the termination of the patent right due to other reasons(Term extension factor has been taken into account ).
Invalid Date:Actual expiry date is based on effective date or publication date of legal transaction data of invalid patent.
Login to view more
IPC IPC(8): A61K35/74A61K31/7008A01K67/02
Inventor 柳国斌韩强
Owner SHUGUANG HOSPITAL AFFILIATED WITH SHANGHAI UNIV OF T C M
Login to view more 
Login to view more