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Method for separating and purifying oligomeric proanthocyanidins

A technology for separation and purification of oligomeric proanthocyanidins, which is applied in the field of separation and purification of plant oligomeric proanthocyanidins, can solve problems such as poor selectivity, high energy consumption, and large loss of active ingredients, and achieve stable performance, low energy consumption, and excellent activity and solubility. good effect

Inactive Publication Date: 2014-01-01
ZHEJIANG UNIVERSITY OF SCIENCE AND TECHNOLOGY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

In this process, the selectivity of oligomeric and high polymeric components is poor, the content of oligomeric components in the product is low, and the loss of active components in the process is large, energy consumption is high, and there is pollution, etc.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0018] Example 1 Separation and purification of oligomeric proanthocyanidins in grape seeds

[0019] The pulverized grape seed raw material is extracted by ethanol solution, after the solvent is recovered, the water sinks and filters, and then the filtrate is subjected to microfiltration treatment. Adjust the pH value of the obtained dialysate to 6, use a specific macroporous resin chromatography column to absorb, wash and separate with water, and elute the chromatography column with a 50% ethanol solution by volume percentage to obtain an eluent containing proanthocyanidins. Concentrate the eluate containing proanthocyanidins with a nanofiltration membrane at a pressure of 0.2 MPa and a temperature of 25° C., and spray dry to obtain the product.

Embodiment 2

[0020] Example 2 Separation and purification of oligomeric proanthocyanidins in pine bark

[0021] The crushed pine bark raw material is extracted by ethanol solution, after the solvent is recovered, the water sinks and filters, and then the filtrate is subjected to microfiltration treatment. Adjust the pH value of the obtained dialysate to 6.5, adopt a specific macroporous resin chromatography column to absorb, wash and separate with water, and elute the chromatography column with a volume percentage concentration of 45% ethanol solution to obtain an eluent containing proanthocyanidins. Concentrate the eluate containing proanthocyanidins with a nanofiltration membrane at a pressure of 0.2 MPa and a temperature of 25° C., and spray dry to obtain the product.

Embodiment 3

[0022] Example 3 Separation and purification of oligomeric proanthocyanidins in purple sweet potato

[0023] The pulverized purple sweet potato raw material is extracted by ethanol solution, after the solvent is recovered, the water sinks and filters, and the filtrate is subjected to microfiltration treatment. Adjust the pH value of the obtained dialysate to 5, adopt a specific macroporous resin chromatography column to absorb, wash and separate with water, and elute the chromatography column with a 40% ethanol solution by volume percentage to obtain an eluent containing proanthocyanidins. Concentrate the eluate containing proanthocyanidins with a nanofiltration membrane at a pressure of 0.1 MPa and a temperature of 30° C., and spray dry to obtain the product.

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PUM

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Abstract

The invention discloses a method for separating and purifying oligomeric proanthocyanidins. The method comprises the following steps: carrying out water precipitation filtration and microfiltration treatment of a proanthocyanidin crude extract liquid, carrying out chromatography adsorption by a specific macroporous resin, and eluting by an ethanol solution to obtain a proanthocyanidin-containing eluate; and carrying out nanofiltration membrane concentration of the eluate, and drying to obtain a product.

Description

technical field [0001] The invention relates to a method for separating and purifying plant oligomeric proanthocyanidins. Background technique [0002] Proanthocyanidins are a class of polyphenols that are formed by connecting monomers such as catechin, epicatechin and epicatechin gallate through carbon bonds, and are widely found in grapes, pine trees and other plants. Proanthocyanidin is a safe and non-toxic pure natural antioxidant, which can remove excess free radicals in the body. As an active ingredient of anti-mutation, anti-cancer, and anti-cardiovascular disease drugs, it is widely used in medicine, health food, daily chemical industry and other fields. In the industry, according to the degree of polymerization of monomers, proanthocyanidins with a degree of polymerization of 7 or more are called high-poly proanthocyanidins (PPC), and those with a degree of polymerization of 2-7 are called oligomeric proanthocyanidins (OPC). Studies have shown that with the increas...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07D311/62
CPCC07D311/62
Inventor 王永江杨志祥毛建卫朱银邦邵云东
Owner ZHEJIANG UNIVERSITY OF SCIENCE AND TECHNOLOGY
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