Method for producing cellulase through fermentation by adopting corn cob as main raw material

A technology of cellulase and main raw materials, which is applied in the field of fermenting cellulase with corn cob as the main raw material, can solve the problem of high cost, and achieve the effects of low cost, low price and significant economic benefit prospect.

Active Publication Date: 2014-01-15
湖南康捷生物科技有限公司
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Problems solved by technology

[0003] However, the research on the preparation of cellulase so far has shown that the cost of raw materials for the production of cellulase is relatively high, and the source of corncobs in rural areas is extensive, which prompts us to explore and study the method of turning waste into treasure and preparing cellulase at low cost. The present invention is exactly under such guiding ideology, obtains success through numerous tests

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  • Method for producing cellulase through fermentation by adopting corn cob as main raw material

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Embodiment 1

[0043] On a sterile ultra-clean bench, take a slant strain, add 10 ml of 0.9% physiological saline, scrape off the spores on the culture medium with a bamboo stick, and obtain the strain spore liquid. Get 1ml bacterial seed spore liquid and put into the 250ml triangle that contains 50ml seed culture medium, place shaker. Culture conditions: rotation speed 200rpm, temperature 28°C, constant temperature cultivation for 50 hours, which is the seed liquid for fermentation and enzyme production.

[0044] Corncobs are prepared into corncob powder through pulverization, according to corncob 20g / L, corn steep liquor 12g / L, (NH4) 2 SO 4 1.2g / L,KH 2 PO 4 2g / L, MgSO 4 7H 2 O0.2g / L, CaCl 2 Mix 5 liters of enzyme-producing medium at a ratio of 0.2g / L, adjust the pH to 5.0 with phosphate, put it into a 7-liter fermenter, add 2ml of antifoaming agent, and sterilize at 121°C for 30 minutes.

[0045] The cultivated seeds are inoculated into a 7-liter fermenter equipped with 5 liters of ...

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Abstract

The present invention provides a method for producing cellulase through fermentation by adopting corn cob as a main raw material, wherein the adopted fermentation strain is preserved in the China Center for Type Culture Collection on June 5, 2012, has a preservation number of CCTCC M 2012204, and is the Trichodema reesei QZ-5 strain. The method is characterized in that agricultural product waste corn cob is adopted as a main raw material to carry out fermentation production to prepare cellulase, the international standard method recommended by the International Union of Pure and Applied Chemistry (IUPAC) is adopted to determine enzyme activity (ie., filter paper enzyme activity FPA determination), the highest enzyme activity of the cellulase prepared through fermentation can be more than 8 IU / ml, the cost is low, the waste is changed into treasure, the method is simple and easy to perform, and the produced cellulase can be widely used in fields of textiles, papermaking, feeds, food and the like, and belongs to the technical field of biology.

Description

technical field [0001] The fermenting bacterial strain that the present invention adopts is the Trichodema reesei QZ-5 cellulase-producing Trichodema reesei QZ-5 that is preserved in China's Type Culture Collection Center on June 5, 2012 with a preservation number of CCTCC M 2012204. The feature of the invention is to ferment and produce cellulase from agricultural product waste-corncobs as the main raw material, and use the international standard method recommended by the International Association of Theoretical and Applied Chemistry (IUPAC) to measure the enzyme activity, that is, the filter paper enzyme activity FPA assay, the highest enzyme activity can be Up to 8IU / ml or more, low cost and can turn waste into treasure, simple and easy. The produced cellulase can be widely used in textile, papermaking, feed, food and other fields, and belongs to the field of biotechnology. Background technique [0002] Cellulose is the most widely distributed, most abundant, and cheapes...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N9/42C12R1/885
CPCC12N9/2437
Inventor 张峰李洪兵张明
Owner 湖南康捷生物科技有限公司
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