Application of Nauclea officinalis extract to preparation of medicament for resisting methicillin-resistant Staphylococcus aureus

A kind of extract, the technology of gallwood, which is applied in the field of new application of the extract of gallwood, can solve the problem that there is no literature report that gallwood is resistant to methicillin-resistant Staphylococcus aureus, and achieves the effect of low production cost and simple preparation process

Active Publication Date: 2014-01-22
SUN YAT SEN UNIV
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

There is no literature report on the application of anti...

Method used

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  • Application of Nauclea officinalis extract to preparation of medicament for resisting methicillin-resistant Staphylococcus aureus
  • Application of Nauclea officinalis extract to preparation of medicament for resisting methicillin-resistant Staphylococcus aureus
  • Application of Nauclea officinalis extract to preparation of medicament for resisting methicillin-resistant Staphylococcus aureus

Examples

Experimental program
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Effect test

Embodiment 1

[0028] Preparation of eucalyptus water extract and its extract

[0029] Gallwood (Nauclea officinalis Pierre ex Pitard) was provided by Hainan Jinguang Pharmaceutical Co., Ltd. Take 3000g of danmu, decoct with 45000ml of water for 2 times, each time for 2 hours, and filter; combine the filtrates and concentrate to obtain 296.5g of extract (water extract). Take extract 197.7g, after suspending with 2000ml of water, extract with petroleum ether, ethyl acetate, n-butanol respectively with 2000ml each time, extract three times with each solvent, combine each extract respectively, concentrate to obtain petroleum ether extract 0.9 g, 20.3 g of ethyl acetate extract, 74.1 g of n-butanol extract, and 102.4 g of aqueous layer extract.

Embodiment 2

[0031] Screening of the Pharmacological Effects of the Water Extract of Panax japonica and Its Extracts Against Methicillin-resistant Staphylococcus Aureus (MRSA)

[0032] 1 Experimental method

[0033] 1) Determination of minimum inhibitory concentration (MIC):

[0034] The water extract and its extract, erythromycin, ceftriaxone sodium, levofloxacin, and vancomycin were respectively diluted in a series of ratios in MH broth medium, and the inoculum was adjusted to 1.0×10 6 CFU / ml. After incubating at 35°C for 24 hours, the lowest concentration of antibacterial drug without precipitation is its minimum inhibitory concentration (MIC).

[0035] 2) Determination of minimum bactericidal concentration (MBC):

[0036] Pipette 50 μl from the aseptic growth well of item 1) onto a blood plate, spread evenly, incubate at 35°C for 24 hours, count the colonies, and reduce the number of viable bacteria in the initial experiment by 99.9% or above. The concentration is its minimum bacte...

Embodiment 3

[0055] Preparation of the 10% ethanol extract of Aloe japonica and its extract

[0056] Gallwood (Nauclea officinalis Pierre ex Pitard) was provided by Hainan Jinguang Pharmaceutical Co., Ltd. 3000g of dandelion wood was extracted twice with 15000ml of 10% ethanol under reflux, each time for 2 hours, and filtered; the combined filtrates were concentrated to obtain 288.3g of extract (10% ethanol extract). Get 192.2g of medicinal extract, extract with petroleum ether, ethyl acetate, n-butanol respectively, each solvent extracts three times, each time 2000ml, combine each extract respectively, concentrate to obtain petroleum ether extract 1.0g, ethyl acetate extract 20.2g, n-butanol extract 72.7g, aqueous layer extract 98.3g.

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Abstract

The invention discloses application of water extract, ethanol extract, ethanol aqueous solution extract of Nauclea officinalis and corresponding petroleum ether abstract, ethyl acetate abstract, n-butanol abstract and water abstract of Nauclea officinalis to preparation of medicaments for resisting methicillin-resistant Staphylococcus aureus (MRSA). At the same time, the invention discloses a preparation method of the above extracts or abstracts. The experimental results show that water extract, ethanol extract, ethanol aqueous solution extract of Nauclea officinalis and corresponding ethyl acetate abstract, n-butyl alcohol abstract and water abstract have strong effect in resisting MRSA, and an ethyl acetate extraction position of 70% ethanol extract of Nauclea officinalis has the highest activity.

Description

technical field [0001] The invention belongs to the field of traditional Chinese medicine pharmacy and relates to a new application of Nauclea officinalis Pierre ex Pitard extract. Background technique [0002] As we all know, current bacterial drug resistance has become a major public health problem worldwide, and the abuse of antibiotics is an important reason for bacterial drug resistance. Methicillin-resistant Staphylococcus aureus (hereinafter referred to as "MRSA") has multi-drug resistance and is associated with high morbidity and mortality. It is one of the important pathogenic bacteria of nosocomial infection and community infection, which brings great difficulties to clinical treatment. MRSA is resistant to the vast majority of antibacterial drugs, and even vancomycin, which is the most effective clinical anti-drug-resistant bacteria, has begun to develop drug resistance. Once MRSA develops to the extent of universal resistance to vancomycin, patients infected wit...

Claims

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Application Information

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IPC IPC(8): A61K36/74A23L1/30A61P31/04A23L33/105
Inventor 王永刚刘博宇苏薇薇姜傥李泮霖欧淑芬
Owner SUN YAT SEN UNIV
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