Nanometer structure and application of water extract of Herba Rabdosiae leaves
A technology of rubescens leaf and nanostructure, applied in the field of biomedicine, can solve the problem of no nanostructure and the like
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Embodiment 1
[0016] Embodiment 1 The preparation of the water extract of the leaves of Oridonium radix
[0017] After washing 21 g of dried Radix Grass leaves with tap water, add 500 ml of double distilled water at 60 - 100 oC, preferably 80 oC, and stir for extraction for 10 - 60 minutes, preferably 30 minutes. The filtered supernatant was concentrated under reduced pressure at 40 oC to obtain 6.3 g water extract of Radix radix L. as light yellow solid, which was ground into powder for future research.
Embodiment 2
[0018] Example 2 The nanostructure of the water extract of the leaves of Oryx sativa
[0019] In order to describe the nanostructure of the water extract of Rubescens leaves, the present invention makes the light yellow solid of the water extract of Rubescens leaves into concentrations of 78.75 mg / ml, 15.75 mg / ml, 3.15 mg / ml and 0.63 mg / ml Then the solution was dropped on the copper grid, evaporated at 35 °C for 24 h to dryness and then operated according to the standard (20 eV energy window, 6000 - 400,000 times) in the transmission electron microscope (JSM- 6360 LV, JEOL, Tokyo, Japan) to observe the nanomorphology. The measured transmission electron microscope pictures are shown in figure 1 – 4 in.
[0020] figure 1 It shows that in the ultrapure aqueous solution with a concentration of 78.75 mg / ml, the water extract of Rubescens leaves exists as aggregates of nanospheres with a diameter of 102.3 nm.
[0021] figure 2 It shows that in the ultrapure aqueous solution...
Embodiment 3
[0025] Example 3 Evaluation of the anti-osteoporosis activity of the water extract of Oryx sativa leaves
[0026] 5-week-old ICR mice, body weight 25.7±2.1g, 48 were randomly divided into ovariectomy group + normal saline (called normal saline group), sham operation group + normal saline (called sham operation group), ovariectomy + 17β-estrogens Diol (called estradiol group), ovariectomized + water extract of Radix radix leaves (called DLC group).
[0027] Intraperitoneally inject 2% pentobarbital sodium at a dose of 40 mg / kg, anesthetize the mice, cut open the abdominal cavity of the mice, find the oviducts on both sides, find the ovaries at the blind ends of the oviducts on both sides, and remove the bilateral ovaries of the mice. Ligate the fallopian tubes (if the mouse wakes up during the operation, dip an appropriate amount of anhydrous ether into the nose of the mouse to anesthetize it). After ligation, a few drops of penicillin was added to the abdominal cavity of th...
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