Chinese herbal medicine composite probiotic feed additive for pigs and preparation method of additive
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A compound probiotic and feed additive technology, applied in animal feed, animal feed, application, etc., can solve the problems of complex components and low utilization rate of beneficial components
Active Publication Date: 2014-02-05
BEIJING CENT BIOLOGY CO LTD
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Problems solved by technology
But at the same time, it also faces problems such as complex components and low utilization rate of beneficial components, so that many functions cannot be fully exerted in the kinetic energy body
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Embodiment 1
[0021] 1. Screen out bacillus, lactic acid bacteria, and yeast that are resistant to 0.3% pig bile salt and acidic environment with a pH of 3.
[0022] 2. The three bacterial strains screened out were subjected to screening experiments for inhibiting pathogenic bacteria. The pathogenic bacteria inhibited are Escherichia coli, Salmonella, and Staphylococcus aureus. The three strains screened out had strong inhibitory effect on pathogenic bacteria.
[0023] 3. Preparation of seed solution:
[0024] Preparation of Bacillus bacteria liquid: Pick a loop from the slant of the test tube, inoculate it into LB medium, and incubate at 160 rpm at 37°C for 16 hours.
[0025] Preparation of lactic acid bacteria liquid: pick a ring from the slant of the test tube, inoculate it into MRS medium, and incubate in a sealed manner at 37°C for 24 hours.
[0026] Yeast liquid preparation: Pick a ring from the slant of the test tube, inoculate it into YPD medium, and incubate in a sealed manner a...
Embodiment 2
[0032] 1. Screen out bacillus, lactic acid bacteria, and yeast that are resistant to 0.3% pig bile salt and acidic environment with a pH of 3.
[0033] 2. The three bacterial strains screened out were subjected to screening experiments for inhibiting pathogenic bacteria. The pathogenic bacteria inhibited are Escherichia coli, Salmonella, and Staphylococcus aureus. The three strains screened out had strong inhibitory effect on pathogenic bacteria.
[0034] 3. Preparation of seed solution:
[0035] Preparation of Bacillus bacteria liquid: Pick a ring from the slant of the test tube, inoculate it into LB medium, and incubate at 180 rpm at 37°C for 16 hours.
[0036] Preparation of lactic acid bacteria liquid: pick a ring from the slant of the test tube, inoculate it into MRS medium, and incubate in a sealed manner at 35°C for 36 hours.
[0037] Yeast liquid preparation: Pick a ring from the slant of the test tube, inoculate it into YPD medium, and incubate in a sealed manner a...
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Abstract
The invention discloses a Chinese herbal medicine composite probiotics feed additive for pigs and a preparation method of the Chinese herbal medicine composite probiotics feed additive. The Chinese herbal medicine composite probiotics feed additive for the pigs consists of a composite Chinese herbal medicine fermentation material, bacillus, saccharomycetes and lactic acid bacteria. The composite Chinese herbal medicine fermentation material comprises the following ingredients in parts by weight: 5-6 parts of Astragalus membranaceus, 2-3 parts of honeysuckle, 1-2 parts of bean pulp and 1-2 parts of bran. The quantity ratio of the bacillus to the lactic acid bacteria to the saccharomycetes is (6-5):(4-3):(2-1), and the total amount of viable bacteria in each gram of product is more than 2.0*109. Chinese herbal medicines are used as fermentation substrates, and three kinds of probiotics are added to perform fermentation, and therefore, the additive contains the effective constituents of the Chinese herbal medicines and the probiotics are enhanced. The Chinese herbal medicine composite probiotics feed additive for the pigs adjusts the microecological balance of the pig intestines, reduces the intestines problems, promotes the growth, enhance the anti-stress capability and immunity of the pigs, reduces the feed conversion ratio, improves the quality of meat, reduces the foul smell of faeces, improves the breeding environment and increases the economic benefits of pig raising.
Description
technical field [0001] The invention relates to the field of feed additives and the technical field of biopharmaceuticals, in particular to a pig feed additive containing Chinese herbal medicine and compound probiotics and a preparation method thereof. Background technique [0002] With the widespread use of antibiotics in feed additives, its disadvantages are becoming increasingly prominent, such as causing animal gastrointestinal flora imbalance, drug resistance and drug residues, etc., triggering a series of public safety, environmental protection and livestock and poultry products Quality issues. In view of this, the voice against adding antibiotics in feed is getting louder and louder. Sweden and Switzerland completely banned the use of antibiotics in feed in 1986 and 1999 respectively; Japan and other countries also imposed strict restrictions on the types, methods of use, dosage and compatibility of antibiotics used in feed; The banning of the last 4 antibiotics (mo...
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